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Ankle2 (NM_027922) Mouse Tagged ORF Clone
CAT＃: MR211330
- TrueORF®
Ankle2 (Myc-DDK-tagged) - Mouse ankyrin repeat and LEM domain containing 2 (Ankle2)
ORF Plasmid: tGFP
"NM_027922" in other vectors (4)
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CNY 7,056.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110001J12Rik; AI661024; D5Ertd585e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211330 representing NM_027922
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGGCAGCGGCTGGCTGTGGTGGAGTGGGCGGCCCTGGCCTGGGAGCTCCTGGGCGCCTCGGTGC TGTTCATCGCCGTGCGCTGGCTGGTCCGCCGGCTAGAGAAGCGGCCGCGGGACCTGAACCGTTGCGGGAC GCTCTCCTCTCCGCCCAGCGCGTCTGAAGCGGTGGCCGCACAGCCAGGTGAAGTGACAATGGATGCCATG ATGGCTCGACTGAAACTCTTGAATCCAGATGACCTTAGAAAAGAAGTTATGAAAGCTGGACTTAAATGTG GACCCATTACATCAACAACAAGATTTATTTTTGAGAAGAAATTAGCTCAGGCCTTACTGGAACAAGGTGG ATTGCTGACCTCTTCCCTTCCCAAGCCCAGCGCGGTTACTGCCATGGCATTTATCCAGGGCACCTCAAGG ACTCCTCCATCTGTGGACGGGAAACAAACTCAGCAGGCCTGTTTTTCTGAAGACAGAGATTTTGGTTACA GTGTGGGCTTGAACCCTCCAGAAGAGGAAGCGGTAGCATCCAGTGTCCACCCAGTACCCTTCAGTGCCTC TACCAGGAATGACAACCACAAGGCTGGAGTGACAGCTCCTAAGGAGCCACTTGTGTACTATGGAGTGTGT CCAGTATATGAGGATGGCCCTGTGAGACATGAGAGGATCCATGTTTACGAAGATAAAAAGGAAGCTTTAC AAGCTGCCAAGTTGATCAAAGGGTCCCGGTTTAAAGCTTTTCGAACAAGAGAAGATGCTGAGAAATTTGC TAGAGGGATATGTGATTATTTACCTTCTCCCAACAAAACTACGCCCTTACTGTCTCCTGTGAAGGCAGTG CCCCTCGGTGGCAGTGATGGGCTGAAAGATGGCTTGTGCCTGGCTGAATCAGAAACAGTAAACAAAGAAC GAGCAAACAGTTACAAAAACCCCCGTACCCAGGACCTCACGGCCAAGCTGCGGAAAGCTGTCGAAAACGG GGAGGAACACACCTTTTCTGATCTTATCTGGAGTAATCCTCGATATCTAATTGGTTCTGGGGACAACCCA ACCATTGTACAAGAAGGATGTAGGTACAATGTCATGCATGTTGCTGCCAAAGAGAACCAGGCTTCCATGT GCCAGCTGACATTAGAGACTCTGGAGAACCCTGAGTTTATGCGTCTGATGTACCCAGATGACAACATGGA CATGCTACAGAAGCGCATCCTCTACGTTGTTGACCTGTATCTTAATACTCCTGACAAAGTGGGCTTTGAT ACACCACTGCATTTTGCTTGTAAATTTGGAAATGTGGATGTAGTCAATGTGCTTTCTTCACACCCTTTGA TTGTGAAAAACCGAAAGAATAAATATGGTAAAACACCTGAAGAAGTAATTTGTGAAAGAAGCCAAAACAA ATCTCCAGCACTGAAAGAAAGGATTAGAGAATACTTAATGGGTCACTACTATGTGCCACTCCTGAGAGCA GAAGACACTTCTCCTGTCATTGGGGAGCTGTGGTCCTCAGACCAGAAAGCTGAAGCCTCAAATACTGCCC ACTGTAGAAGCAGCCCCAGAGATCCTGTGATGACACTGAGAGCTTTCGTGGGACCTCTGAGTCCATCCAA AGCAGAAGATTTTCGAAAGCTCTGGAAAACTCCACCTCGAAAGAAAGCAGGCTTCTTCCACAGCATCAGA AAGTCTGACCCAGAACGAGGCATTGAGAGAGTTGGAAGGGAGCTAGCTCATGAGCTGGGGTATCCCTGGG TTGAATACTGGGAATTTCTGGGATGTTTTGTTGATCTGTCTTCCCAGGAAGGCTTGCAAAGACTAGAAGA ATACCTTATCCAGAAGGAGTTAAGCAAAAAGGCTCAACAAGAAATACGAGAAAATGAAGGCTGCCTTCAG GACAGAACCTCAGATTTTGGCAGTGGAAAGAAGTACAGCAACTCCATCTCCGTGGGAGCATTTCTAGATG GGGATGATGACAGTAGTTTGGAAGAAATTAAAAACCAGCAAAATACAGTTCCAAGTCAGAGTCAGCCCAC AACTGATAAGTTCCAAACCTCTAAAAGTGGTTCCCTTCCCTTGGGACAGAAGGTCGACCCTGGAGAAACC TCAGTTGGAACTTACCCAGATAAAGGCAGAAATGGGTTTTGTCACCCTCTAAACCACAGGACTGCAGATG GAAGGGGACTAGAGGCCACCAATGGGGAGGAGGCCCTTCCACCACCTGTTTCTGTTTTGACTCAAGAGTT GAATAAACTGAATTTGCAAAGCCTGGGAGACAGCCTTCATGAGACACCAGATAAAAACGGGAAACTAGAG GATGAGGTGCTTCCTTCAAGAAAGGGTGCAGCAGACAGTGACTTGCTGGCATCTCCTCCTGCCATAGCAA GCCTTGGAAAGAAGCAAGTCAGGACCAACACTGAGGTGTCAGAGGCAATGGCTGAAATGTCTTTGGGCCC TAAGAGCCCCCAACTTGGAGTTCAGGCTGGTCTGGAACCTATACTCTCATCTGCCACGGTAGATTCCACC AAGAGGCTCTTCCTGTCTGGAGAGGAACCATCAAAACTAGATCGAGATGTTTTAGCAGCTCTTGAGTGTG CGAATATTGACCCTGGTCTGTACCCAGCAATCCACAGATGGAAAAGCACTGTCATGTGCTACTCGCCCTC AGACAGACAGAGTTGGCCAAGTCCTGCACTCAAAGGGAAGTTCACAACTGAGTTGGTGGATCTCGATTGC TCTCATAGCTGCTCAGGACGATGCAGTCCAGCTGGTAGCAGCCCCAGTAAGCCTGGCCACACCTCCTCCT CTTCTGGCCTGCACAGCCCTGGACGCTACAGTCCAGCACATGGGAGACACTTCCAAAGAGTGGCACACGT GGCCCGACTTGCAGCTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211330 representing NM_027922
Red=Cloning site Green=Tags(s) MLWQRLAVVEWAALAWELLGASVLFIAVRWLVRRLEKRPRDLNRCGTLSSPPSASEAVAAQPGEVTMDAM MARLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQGGLLTSSLPKPSAVTAMAFIQGTSR TPPSVDGKQTQQACFSEDRDFGYSVGLNPPEEEAVASSVHPVPFSASTRNDNHKAGVTAPKEPLVYYGVC PVYEDGPVRHERIHVYEDKKEALQAAKLIKGSRFKAFRTREDAEKFARGICDYLPSPNKTTPLLSPVKAV PLGGSDGLKDGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVENGEEHTFSDLIWSNPRYLIGSGDNP TIVQEGCRYNVMHVAAKENQASMCQLTLETLENPEFMRLMYPDDNMDMLQKRILYVVDLYLNTPDKVGFD TPLHFACKFGNVDVVNVLSSHPLIVKNRKNKYGKTPEEVICERSQNKSPALKERIREYLMGHYYVPLLRA EDTSPVIGELWSSDQKAEASNTAHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGFFHSIR KSDPERGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELSKKAQQEIRENEGCLQ DRTSDFGSGKKYSNSISVGAFLDGDDDSSLEEIKNQQNTVPSQSQPTTDKFQTSKSGSLPLGQKVDPGET SVGTYPDKGRNGFCHPLNHRTADGRGLEATNGEEALPPPVSVLTQELNKLNLQSLGDSLHETPDKNGKLE DEVLPSRKGAADSDLLASPPAIASLGKKQVRTNTEVSEAMAEMSLGPKSPQLGVQAGLEPILSSATVDST KRLFLSGEEPSKLDRDVLAALECANIDPGLYPAIHRWKSTVMCYSPSDRQSWPSPALKGKFTTELVDLDC SHSCSGRCSPAGSSPSKPGHTSSSSGLHSPGRYSPAHGRHFQRVAHVARLAAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027922 |
| ORF Size | 2889 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027922.2, NP_082198.1 |
| RefSeq Size | 5256 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 71782 |
| UniProt ID | Q6P1H6 |
| MW | 106.6 kDa |
| Gene Summary | Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202569 | Ankle2 (untagged) - Mouse ankyrin repeat and LEM domain containing 2 (Ankle2), (10ug) |
CNY 7,064.00 |
|
| MG211330 | Ankle2 (tGFP-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 585, expressed (D5Ertd585e) |
CNY 7,890.00 |
|
| MR211330L3 | Lenti ORF clone of Ankle2 (Myc-DDK-tagged) - Mouse ankyrin repeat and LEM domain containing 2 (Ankle2) |
CNY 9,030.00 |
|
| MR211330L4 | Lenti ORF clone of Ankle2 (mGFP-tagged) - Mouse ankyrin repeat and LEM domain containing 2 (Ankle2) |
CNY 9,030.00 |
