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Kif5b (NM_008448) Mouse Tagged ORF Clone
CAT＃: MR211329
- TrueORF®
Kif5b (Myc-DDK-tagged) - Mouse kinesin family member 5B (Kif5b)
ORF Plasmid: tGFP
"NM_008448" in other vectors (4)
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CNY 10,576.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AL022807; Khc; Khcs; Kns1; Ukhc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211329 representing NM_008448
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCCGGCGGAGTGCAACATCAAAGTGATGTGTCGCTTCAGACCTCTCAACGAATCTGAAGTGA ACCGCGGCGATAAGTACGTCGCCAAATTCCAGGGAGAAGACACGGTGATGATCGCGTCCAAGCCTTATGC CTTTGATCGTGTGTTCCAGTCAAGCACATCTCAAGAGCAAGTATACAACGACTGTGCAAAAAAGATTGTT AAAGATGTTCTTGAGGGCTATAATGGAACAATATTTGCATATGGACAAACATCATCTGGGAAGACCCACA CGATGGAGGGTAAACTTCATGATCCAGAAGGCATGGGAATTATTCCAAGAATAGTGCAAGATATTTTTAA TTATATTTACTCCATGGATGAAAATTTGGAATTTCATATTAAGGTTTCATATTTTGAAATATATTTGGAT AAGATAAGGGACTTGTTAGATGTTTCAAAGACTAACCTTTCAGTCCATGAAGACAAAAACCGTGTTCCCT ATGTAAAGGGGTGCACAGAACGTTTCGTGTGTAGTCCAGATGAAGTCATGGATACCATAGATGAAGGGAA ATCCAACAGACATGTCGCAGTTACAAATATGAATGAACATAGCTCTAGGAGTCACAGCATATTTCTTATT AATGTAAAACAAGAGAATACACAAACGGAACAGAAACTCAGTGGAAAGCTTTATCTGGTTGATTTAGCTG GCAGTGAGAAGGTTAGTAAGACTGGGGCTGAAGGTGCTGTGCTGGATGAAGCTAAGAACATCAACAAGTC ACTTTCTGCACTTGGAAATGTCATTTCTGCTTTGGCAGAGGGCAGTACCTATGTTCCTTATCGAGATAGT AAAATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTC CATCATCATACAATGAGTCTGAGACAAAGTCAACACTCCTCTTTGGTCAAAGGGCCAAAACAATTAAGAA CACAGTCTGTGTCAATGTAGAGTTAACTGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAA AATAAAACTCTACGGAACACTATTCAGTGGCTGGAAAACGAGCTAAACCGTTGGCGTAACGGGGAGACAG TGCCTATTGATGAGCAGTTTGACAAAGAGAAAGCTAATTTGGAAGCCTTCACAGCGGATAAAGATATTGC TATTACCAGTGATAAACCAGCTGCTGCAGTCGGAATGGCTGGTAGTTTTACCGATGCTGAAAGAAGAAAG TGTGAAGAAGAACTTGCTAAATTGTATAAACAACTTGATGACAAGGATGAAGAGATTAACCAACAAAGCC AATTGGTAGAGAAATTGAAGACACAAATGCTGGATCAGGAAGAGCTTCTGGCATCAACCAGAAGGGATCA AGATAATATGCAAGCTGAACTGAATCGCCTCCAAGCAGAAAATGATGCTTCTAAAGAAGAAGTCAAAGAA GTTTTACAGGCCTTAGAGGAACTGGCTGTTAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACAA AGGAATATGAATTGCTTAGTGATGAATTGAATCAAAAATCTGCAACTTTAGCAAGTATTGATGCTGAGCT TCAGAAGCTGAAGGAAATGACCAACCACCAGAAGAAACGAGCAGCTGAAATGATGGCATCATTATTAAAA GACCTTGCAGAAATAGGAATTGCTGTGGGGAATAACGATGTGAAGCAACCAGAAGGAACTGGTATGATAG ATGAAGAGTTTACTGTTGCAAGACTCTACATTAGCAAAATGAAATCAGAAGTAAAGACCATGGTGAAACG CTGCAAACAGCTAGAAAGCACACAGACTGAGAGCAACAAAAAAATGGAAGAAAATGAGAAAGAGTTAGCA GCATGCCAGCTTCGGATCTCCCAACATGAAGCCAAAATCAAGTCACTGACTGAGTACCTTCAGAATGTAG AACAAAAGAAGAGGCAGCTGGAGGAGTCTGTTGATTCCCTTGGTGAGGAGCTAGTCCAACTCCGAGCACA AGAGAAAGTCCATGAAATGGAAAAAGAGCACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCT GTTGAGCAGCAGATCCAGAGTCACAGAGAAACCCACCAAAAACAAATCAGTAGTTTGCGAGATGAAGTGG AGGCAAAGGAAAAGCTAATCACTGACCTCCAAGACCAAAACCAGAAGATGGTGTTGGAGCAGGAACGGCT AAGGGTGGAGCATGAGAGGCTGAAGGCTACAGACCAAGAGAAGAGCAGGAAGCTGCACGAGCTCACGGTT ATGCAAGACAGACGAGAACAAGCAAGACAAGACTTGAAGGGTTTGGAGGAGACCGTGGCAAAAGAACTTC AGACTTTACACAACCTGCGTAAGCTCTTTGTTCAGGACTTGGCTACCAGGGTGAAAAAGAGCGCCGAGGT CGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAA CAGCTCACCAAAGTGCACAAGCAGTTGGTACGCGATAATGCAGATCTTCGCTGTGAGCTTCCTAAGTTAG AGAAACGGCTTAGAGCTACTGCAGAAAGAGTGAAAGCTTTGGAGTCAGCCCTGAAAGAAGCCAAAGAAAA TGCATCTCGAGACCGTAAACGCTATCAGCAAGAAGTAGACCGGATAAAGGAAGCAGTCAGGTCAAAGAAC ATGGCCAGAAGGGGACATTCTGCCCAGATTGCAAAGCCGATCCGTCCTGGACAGCATCCAGCGGCCTCGC CAACTCACCCGGGCACAGTTCGTGGAGGAGGCTCATTTGTTCAGAACAACCAGCCAGTGGGGCTTCGTGG TGGTGGAGGCAAGCAGTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211329 representing NM_008448
Red=Cloning site Green=Tags(s) MADPAECNIKVMCRFRPLNESEVNRGDKYVAKFQGEDTVMIASKPYAFDRVFQSSTSQEQVYNDCAKKIV KDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLD KIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLI NVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDS KMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEK NKTLRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTADKDIAITSDKPAAAVGMAGSFTDAERRK CEEELAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKE VLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLK DLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELA ACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDSLGEELVQLRAQEKVHEMEKEHLNKVQTANEVKQA VEQQIQSHRETHQKQISSLRDEVEAKEKLITDLQDQNQKMVLEQERLRVEHERLKATDQEKSRKLHELTV MQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEVDSDDTGGSAAQKQKISFLENNLE QLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKN MARRGHSAQIAKPIRPGQHPAASPTHPGTVRGGGSFVQNNQPVGLRGGGGKQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008448 |
| ORF Size | 2889 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008448.3, NP_032474.2 |
| RefSeq Size | 6030 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 16573 |
| UniProt ID | Q61768 |
| MW | 110 kDa |
| Gene Summary | Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence (PubMed:9657148). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (PubMed:21976701). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222865 | Kif5b (untagged) - Mouse kinesin family member 5B (Kif5b), (10ug) |
CNY 12,350.00 |
|
| MG211329 | Kif5b (tGFP-tagged) - Mouse kinesin family member 5B (Kif5b) |
CNY 10,550.00 |
|
| MR211329L3 | Lenti ORF clone of Kif5b (Myc-DDK-tagged) - Mouse kinesin family member 5B (Kif5b) |
CNY 11,500.00 |
|
| MR211329L4 | Lenti ORF clone of Kif5b (mGFP-tagged) - Mouse kinesin family member 5B (Kif5b) |
CNY 11,500.00 |
