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Fgd1 (NM_008001) Mouse Tagged ORF Clone
CAT＃: MR211315
- TrueORF®
Fgd1 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1)
ORF Plasmid: tGFP
"NM_008001" in other vectors (4)
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CNY 7,032.00
CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZFYVE; ZFYVE3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211315 representing NM_008001
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGGCCACCGAGTCCCGGGGGGCCCTGGGCCTTCGGATCCCGAACGCTCAGCGGCAAATACCCCCG GTGCCGCTCCGCTGGCCTGTGCCGACTCGGACCCTGGAGCCTTGGAACCCGGGCTGCCGGTGAGCAGGGG CTCAGGCACGGCTCTTGGAGGCCCTTTGGATCCTCAGTTTGTTGGACCCTCGGATGCCAGCCTGGGAGCG CCTCCAAGCTCCCGGGTCTTACCCTGCGGCCCTAGTCCTCAGCACCACCGGGCCCTGCGCTTCTCCTACC ACCTGGAGGGCTCGCAGCCTCGGCCTGGACTGCACCAGGGAAACCGGATCCTGGTTAAAAGTTTGTCCCT TGACCCTGGCCAGAGCCTTGAGCCTCATCCAGAAGGTCCCCAACGGCTTCGCTCAGATCCAGGCCCTCCC ACTGAAATCCCTGGCCCACGTCCTTCACCACTGAAGCGGGCACCGGGCCCAAAGCCACAGGTTCCCCCAA AGCCCAGCTACCTGCAGATGCCCCGGGTGCTTCCTCCTCCTGAGCCCATTCCTCCACCACCATCACGCCC TCTGCCTGCAGACCCTAGAGTGGCCAAGGGACTCGTCCCCAGAGCTGAGGCCAGCACTAGTTCTGCAGCA GTATCATCATTGATTGAGAAGTTTGAAAGAGAGCCTGTGATTGTTGCCTCGGATAGGCCAGCCCCTGGCC CCTGCCCAGTTCCCCCAGAGCCAGCCATGTTGCCACAGCCACCCCCGCAGCCAACAGGGTCCCAGCTCCC TGAGGGTGAAGCTTCTCGCTGCCTGTTCCTGCTGGCTCCTGGGCCCCGGGATGGTGAGAAGGTGCCTAAC CGGGACAGCGGCATTGACAGCATCAGCTCGCCATCCAACAGTGAAGAGACCTGCTTCGTCAGTGATGATG GGCCCCCCATCCACAGCCTCTGTCCTGGGCCCCCCGCCCTGGCTAGTATGCCAGTAGCTTTGGCCGACCC CCACCGGCCTGGCTCCCAGGAGGTTGACAGTGACCTGGAAGAGGAAGAGGAAGAAGAAGAGGAGGAAAAG GAAAGAGAAATTCCAGTGCCCCCGATGGAGAGACAGGAGTCTGTGGAGCTGACTGTGCAGCAGAAGGTGT TCCATATTGCCAATGAGCTCCTGCAAACTGAGAAGGCCTATGTTTCCAGGCTCCATCTCCTGGATCAGGT ATTCTGTGCCCGGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCCCGCAGATGTTGTCCACGGCATC TTCTCTAACATCTGCTCCATCTATTGCTTCCACCAGCAGTTCCTGCTGCCTGAGCTAGAGAAGCGCATGG AGGAATGGGACCGCTACCCACGCATTGGTGACATTCTTCAGAAGTTGGCACCCTTCCTCAAGATGTATGG CGAGTATGTGAAAAACTTCGACCGGGCGGTAGAGCTGGTCAACACATGGACAGAGCGGTCTACCCAGTTT AAAGTCATCATCCATGAAGTACAGAAGGAGGAAGCCTGTGGCAACCTGACCTTGCAGCACCACATGCTGG AGCCCGTGCAGCGTATCCCCCGCTATGAACTTCTTCTCAAGGACTATCTGTTAAAGCTGCCTCATGGCTC CCCGGACAGCAAGGATGCCCAGAAGTCTTTGGAGCTGATAGCCACAGCAGCAGAACACTCTAATGCTGCC ATCCGGAAAATGGAACGAATGCACAAGCTGCTGAAGGTGTATGAGCTGCTAGGGGGTGAGGAGGACATTG TCAGTCCCACCAAAGAGCTCATAAAGGAAGGCCATATCCTTAAGCTGTCAGCAAAGAATGGGACCACTCA AGATCGATACCTCATATTATTCAACGACCGCCTCCTTTACTGCGTGCCCAGGCTGCGGCTCCTTGGTCAG AAGTTTAGCGTGCGGGCACGCATTGATGTAGATGGCATGGAGCTAAAGGAGAGCTCCAACCTCAACATGC CTCGGACCTTCCTGGTGTCAGGAAAGCAGCGCTCTCTCGAACTGCAGGCCAGGACTGAGGAGGAGAAGAA AGACTGGGTCCAGGCCATCAATTCCACCCTCCTGAAGCATGAACAGACCCTGGAGACTTTCAAACTGTTG AACTCAACAAACAGGGATGATGAAGATACTCCCCCTAACTCTCCAAACGTGGATCTTGGGAAGAGGGCAC CTACGCCTATCCGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAGGAGCCCTTCAATTCCATCACCAA ACGCAGGCACCACTGCAAGGCCTGTGGGCATGTGGTTTGTGGGAAATGCTCTGAGTTCCGAGCCCGACTC ATCTATGACAACAATCGTTCCAACCGTGTGTGCACTGATTGCTATGTGGCCTTGCATGGAGCACCTGGGA GCAGTCCAGCCTGTAGCCAGCATACACCCCAGCGCAGGAGGTCCATCCTAGAGAAACAGGCCTCGGTCGC TGCTGAGAACAGCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGGTGGGAAAGGATGGCACAAGGCA TGGTTTGTGGTTCCTGAGAATGAACCCTTGGTGCTGTACATCTATGGAGCCCCTCAGGATGTGAAAGCCC AGCGCAGCCTGCCCCTCATTGGCTTTGAGGTGGGGCCTCCTGAGGCAGGAGAGCGGCCAGACAGGAGGCA TGTCTTCAAGATCACACAAAGCCACCTAAGCTGGTACTTCAGCCCAGAGACAGAAGAACTCCAGCGGCGC TGGATGGCTGTTCTCGGCAGGGCGGGCCGTGGGGACACATTCTGCCCAGGGCCCACACTGTCTGAGGACA AGGAGATGGAGGAGACACCAGTGGCAGCCTCAGGAGCCACTGCTGAACCTCCTGAAGCCTCCCAGACCCG AGACAAGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211315 representing NM_008001
Red=Cloning site Green=Tags(s) MHGHRVPGGPGPSDPERSAANTPGAAPLACADSDPGALEPGLPVSRGSGTALGGPLDPQFVGPSDASLGA PPSSRVLPCGPSPQHHRALRFSYHLEGSQPRPGLHQGNRILVKSLSLDPGQSLEPHPEGPQRLRSDPGPP TEIPGPRPSPLKRAPGPKPQVPPKPSYLQMPRVLPPPEPIPPPPSRPLPADPRVAKGLVPRAEASTSSAA VSSLIEKFEREPVIVASDRPAPGPCPVPPEPAMLPQPPPQPTGSQLPEGEASRCLFLLAPGPRDGEKVPN RDSGIDSISSPSNSEETCFVSDDGPPIHSLCPGPPALASMPVALADPHRPGSQEVDSDLEEEEEEEEEEK EREIPVPPMERQESVELTVQQKVFHIANELLQTEKAYVSRLHLLDQVFCARLLEEARNRSSFPADVVHGI FSNICSIYCFHQQFLLPELEKRMEEWDRYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQF KVIIHEVQKEEACGNLTLQHHMLEPVQRIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNAA IRKMERMHKLLKVYELLGGEEDIVSPTKELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRLLGQ KFSVRARIDVDGMELKESSNLNMPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKLL NSTNRDDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRARL IYDNNRSNRVCTDCYVALHGAPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHKA WFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQRR WMAVLGRAGRGDTFCPGPTLSEDKEMEETPVAASGATAEPPEASQTRDKT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008001 |
| ORF Size | 2880 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008001.4, NP_032027.2 |
| RefSeq Size | 3516 bp |
| RefSeq ORF | 2883 bp |
| Locus ID | 14163 |
| UniProt ID | P52734 |
| MW | 106.8 kDa |
| Gene Summary | This gene encodes a member of family of Rho-specific guanine nucleotide exchange factors. Rho-specific guanine nucleotide exchange factors catalyze the exchange of GDP for GTP and activate small GTPases, which function as molecular switches in signaling. This protein specifically binds cell division cycle 42, a Rho (Ras homology) GTPase. Investigations in mouse suggest that this protein is important for skeletal mineralization and for regulating the actin cytoskeleton. In humans, mutations in this gene are associated with faciogenital dysplasia, also known as Aarskog-Scott syndrome. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222827 | Fgd1 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1), (10ug) |
CNY 12,260.00 |
|
| MG211315 | Fgd1 (tGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1) |
CNY 9,310.00 |
|
| MR211315L3 | Lenti ORF clone of Fgd1 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1) |
CNY 10,360.00 |
|
| MR211315L4 | Lenti ORF clone of Fgd1 (mGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1) |
CNY 10,360.00 |
