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Vps8 (BC023914) Mouse Tagged ORF Clone
CAT＃: MR211306
- TrueORF®
Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38203 IMAGE:5323126)
ORF Plasmid: tGFP
"BC023914" in other vectors (4)
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CNY 7,016.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mKIAA0804, MGC38232 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211306 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATCTCGGAAGTCCAGCTGGTCTACAACAGCAGCCACTTCAAGTCATTGGCCACGGGAGGAAATG TTAGCCAGGCCCTGGCTTTGGTTGGAGAGAAGGCTTGCTACCAATCCATCAGTAGCTATGGAGGACAGAT CTTCTATCTGGGGACCAAGTCTGTGTACGTGATGATGCTGAGAAGCTGGAGGGAGAGGATGGACCATCTC CTGAAACAGGATTGCCTTACGGAAGCCTTGGCCCTCGCGTGGTCTTTTCATGAAGGAAAGGCAAAAGCAG TAGTGGGGCTATCGGGGGATGTCAGCAAGAGGAAGGCTGTTGTTGCTGATCGGATGGTAGAGATCCTGTT CCATTATGCAGATCGGGCTCTGAAAAAGTGCCCGGACCAGGGGAAAATCCAAGTGATGGAGCAACACTTT CAGGATACGGTGCCAGTCATAGTGGACTACTGCCTTCTGCTCCAGCGAAAGGATCTTCTATTTGGTCAAA TGTACGACAAATTAAGTGAAAATTCCGTCGCAAAAGGCGTGTTTTTGGAGTGCCTTGAGCCATATATCTT AAGTGATAAACTGGTGGGGATTACTCCCCAGGTCATGAAGGACTTAATTGTTCATTTCCAAGATAAAAAA CTGCTGGAGAATGTGGAAGCCCTCATTGTCCACATGGACATCACCAGCCTCGACATTCAGCAGGTGGTCC TCATGTGTTGGGAGAATCGTCTGTATGACGCCATGGTCTATGTCTACAACCGAGGCATGAATGAGTTTAT CAGTCCGATGGAGAAGCTTTTCAAAGTCATCGCCCCACCTCTGAATGCAGGGAAGACTCTTACAGATGAG CAAGTTGTTATGGGCAACAAGCTTCTGGTGTACATCAGCTGTTGCCTGGCAGGTCGTGCCTACCCTCTCG GTGACATTCCTGAAGATCTTGTTCCCTTGGTTAAAAACCAGGTTTTTGAGTTTCTGATTCGCCTGCATTC TGTAGAAGCATCCTCCGAGGAAGAAGTCTATCCTTATGTTCGGACTTTGCTACATTTTGACACAAGAGAA TTTCTGAATGTGCTGGCACTGACCTTTGAAGACTTTAAAAATGATAAGCAAGCTGTGGAATACCAGCAGC GCATTGTGGATATTTTATTGAAGGTTATGGTGGAGAATTCAGATTTTACCCCGTCACAAGTGGGGTGTCT CTTTACGTTCCTCGCTCGTCAGCTCGCAAAGCCTGACAATACCTTGTTTGTGAACAGGACCCTCTTTGAT CAAGTCCTCGAGTTCCTCTGTAGCCCCGACGATGACTCCCGCCACTCTGAAAGACAGCAGGTCCTTTTAG AATTGCTGCAGGCCGGAGGCATAGTTCAATTTGAAGAGAGTCGGCTCATCCGCATGGCAGAAAAGGCAGA ATTCTATCAAATCTGTGAATTTATGTATGAGCGGGAACACCAGTATGACAAGATCATTGACTGCTACCTG CACGACCCATTGAGAGAGGAAGAAGTCTTCAATTATATCCACAACATCTTATCTATTCCTGGTCACAGTG CTGAGGAGAAGCAGTCTGTGTGGCAGAAAGCAATGAATCATATGGAGGAACTTGTGTCCCTGAAGCCCTG CAAAGCCGCAGAGCTGGTGGCTACTCACTTTTCTGAGCAGATCGAAGTGGTCATTGGACAGCTTCAGAAC CAGCTTTTGCTTTTCAAATTTTTGAGGAGTCTTCTTGATCCAAGGGAAGGTGTTCATGTCAATCAGGAGT TGCTGCAGATCCCGCCTCACATTACAGAGCAGTTCATTGAGCTGCTGTGCCAGTTCAGTCCTGACCAAGT CATACAGACACTGCAGGTCCTTGAGTGCTACCGTCTGGAGGAAACGATTCAGATTACACAAAAGTATCAA CTCCATGAAGTCACTGCGTATCTGCTGGAGAAGAAAGGGGATGCACACGGCGCCTTCCTGCTGCTGCTGG AGAGACTGCAGAGCAGGCTGCAAGAGATGACACGGCAGGACGAAAATACCAAGGAGGACATCCTGCTGAA AGGTGTTGAAGATACCATGGTAGAGACAATTGCTCTTTGCCAGAGAAATTCACAGAATTTGAATCAGCAG CAACGAGAGGCTCTATGGTTTCCACTGTTGGAGGCAATGATGACACCACAGAAGCTGTCCAGCTCGGCTG CTGCTCCTCATCCGCACTGTGAAGCTCTGAAGTCTTTGACCATGCAAGTCCTAAACAGCATGGCAGCGTT CATCGCCCTCCCGTCCATCCTACAGAGAATCTTGCAGGACCCAATTTATGGAAAAGGAAAGCTTGGAGAA ATCCAGGGCCTTATTCTGGGGATGCTGGACACCTTCAACTATGAGCAAACCTTGCTAGAAACAACAGCCA GCCTCCTGAACCAAGATCTCCATTGGTCACTGTGTAACCTGAGAGCATCAGTATCCAGAGGACTCAATCC CAAGCAGGATTACTGTTCTATATGTTTACAGCAGTACAAAAGACGCCAAGAAATGGCTGATGAAATTATT GTCTTTAGCTGTGGCCATTTGTATCATTCATTCTGTCTCCAAAGTAAGGAATGCACCCTAGAGGTTGAGG GTCAGACGAGATGGGCGTGTCACAAATGCAGCTCAAGTAATAAAGCGGGCAAACTCAGTGAAAATCCTTC TGAAAACAAGAAAGGACGGATAACCTCGTCTCAGGCAAACTCAGAACCTGTTCTGGACCCACAGCAAATG CAAGCCTTTGATCAGCTCTGCCGTCTCTACAGAGGAAGTTCTAGGAGAGCAAGCCGGAAGCCTATTTCCT CCTTCAGAAGGCCATCCTGTATATCTGAGACTGCCAAGCCTGCCCTACAGCCAGCCCCCCCCTCCACATC TGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211306 protein sequence
Red=Cloning site Green=Tags(s) MEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERMDHL LKQDCLTEALALAWSFHEGKAKAVVGLSGDVSKRKAVVADRMVEILFHYADRALKKCPDQGKIQVMEQHF QDTVPVIVDYCLLLQRKDLLFGQMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKK LLENVEALIVHMDITSLDIQQVVLMCWENRLYDAMVYVYNRGMNEFISPMEKLFKVIAPPLNAGKTLTDE QVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSVEASSEEEVYPYVRTLLHFDTRE FLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFD QVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEREHQYDKIIDCYL HDPLREEEVFNYIHNILSIPGHSAEEKQSVWQKAMNHMEELVSLKPCKAAELVATHFSEQIEVVIGQLQN QLLLFKFLRSLLDPREGVHVNQELLQIPPHITEQFIELLCQFSPDQVIQTLQVLECYRLEETIQITQKYQ LHEVTAYLLEKKGDAHGAFLLLLERLQSRLQEMTRQDENTKEDILLKGVEDTMVETIALCQRNSQNLNQQ QREALWFPLLEAMMTPQKLSSSAAAPHPHCEALKSLTMQVLNSMAAFIALPSILQRILQDPIYGKGKLGE IQGLILGMLDTFNYEQTLLETTASLLNQDLHWSLCNLRASVSRGLNPKQDYCSICLQQYKRRQEMADEII VFSCGHLYHSFCLQSKECTLEVEGQTRWACHKCSSSNKAGKLSENPSENKKGRITSSQANSEPVLDPQQM QAFDQLCRLYRGSSRRASRKPISSFRRPSCISETAKPALQPAPPSTSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023914 |
| ORF Size | 2874 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023914, AAH23914 |
| RefSeq Size | 3383 bp |
| RefSeq ORF | 2876 bp |
| Locus ID | 209018 |
| MW | 109.5 kDa |
| Gene Summary | Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220893 | Vps8 (untagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38203 IMAGE:5323126), (10ug) |
CNY 9,120.00 |
|
| MG211306 | Vps8 (tGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38203 IMAGE:5323126) |
CNY 5,990.00 |
|
| MR211306L3 | Lenti ORF clone of Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38203 IMAGE:5323126) |
CNY 7,320.00 |
|
| MR211306L4 | Lenti ORF clone of Vps8 (mGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38203 IMAGE:5323126) |
CNY 7,320.00 |
