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Med24 (NM_011869) Mouse Tagged ORF Clone
CAT＃: MR211298
- TrueORF®
Med24 (Myc-DDK-tagged) - Mouse mediator complex subunit 24 (Med24)
ORF Plasmid: tGFP
"NM_011869" in other vectors (4)
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CNY 7,232.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 100kD; 911GSE; AU040102; AW547152; D11Ertd307; D11Ertd307e; DRIP; DRIP100; Gse; Gse2; Pp; Pparb2; Pparbp2; R7552; R75526; Thr; Thrap4; Trap; Trap100 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211298 representing NM_011869
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGGTGAACCTGAAGCAGGCCATTTTGCAAGCCTGGAAAGAGCGATGGAGTGACTACCAATGGG CAATCAACATGAAAAAATTCTTCCCCAAAGGAGCCACCTGGGACATTCTCAACCTAGCAGAAGCGCTCCT GGAGCAGGCTATGATTGGGCCTTCCCCCAATCCTCTGATCCTGTCGTACCTGAAGTACGCCATTAGTTCC CAGATGGTGTCCTGCTCCTCTGTGCTCACCGCCATCAGTAAGTTTGATGACTTTTCCCGGGACCTGTGTG TCCAGGCTCTGCTGGATATCATGGACATGTTCTGTGACCGACTCAGTTGCCACGGCAAAGCAGAGGAATG CATCGGGTTGTGCCGAGCGCTCCTTAGCGCCCTCCACTGGCTGCTTCGATGTACAGCAGCTTCTGCGGAG CGACTCCAGGAAGGGCTGGAGGCTGGCACTCCGGCCCCCGGGGAGAAGCAGCTTGCCTTGTGCTTGCAGT GCCTGGAGAAGACCCTCAGCAGCACCAAGAACCGAGCCCTGCTACACATCGCCAAACTAGAGGAGGCCTC CTCCTGGACTGCCATAGAGCATTCTCTTCTAAAACTTGGGGAGATCCTGGCCAATCTCAGCAACCCCCAG CTCCGGAGCCAAGCTGAGCGTTGCGGCACACTCATCAGGAGCATCCCCAGCATGCTGTCCGTGCACTCCG AGCAACTGCACAAGACCGGCTTCCCCACCATCCACGCACTCATCTTGCTTGAGGGCACCATGAACCTGAC CGGGGAGATGCAGCCCCTGGTGGAGCAGCTGATGATGGTGAAGCGCATGCAGCATATCCCCACACCTCTC TTTGTCCTGGAAATCTGGAAAGCTTGCTTTGTGGGACTCATTGAGTCCCCCGAGGGCACGCAGGAGCTGA AGTGGACAGCTTTCACCTACCTCAAGATTCCACAGGTCTTGGTGAAGTTGAAGAAGTACTTTCATGGAGA GAAGGATTTCACGGAGGATGTCAACTGTGCTTTTGAGTTCCTGCTGAAGCTCACCCCCTTGTTGGACAAA GCTGACCAGCGCTGCAATTGCGACTGCACGAACTTCCTCCTCCAGGAGTGCAACAAGCAGGGGCTTCTGT CTGAGGTCAACTTTGCCAGCCTTGTGGGCAAACGCACGGCAGATCGGGACCCGCAGCTAAAATCATCAGA AAATGCTAACATCCAGCCGAATCCTGGCCTGATCCTGCGGGCAGAGCCCACGGTCACAAACATTCTCAAG ACAATGGATGCAGATCACTCCAAGTCCCCGGAGGGACTGCTAGGGGTCCTGGGACATATGCTGTCTGGGA AGAGCCTGGATTTGCTGCTGGCTGCTGCCGCCGCCACTGGGAAGCTTAAATCCTTCGCCCGGAAATTCAT CAATCTGAATGAGTTCACGACCCACGGCAGTGGAGAGAGCACGAAAACAGCCTCGGTCCGAGCCTTGCTC TTTGACATTTCCTTCCTCATGCTATGCCATGTGGCCCAGACCTATGGCTCAGAGGTGATCCTCTCGGAGT CGAGCTCAGGAGAAGAAGTCCCCTTCTTTGAGACTTGGATGCAGACCTGCATGCCCGAGGAGGGCAAGAT TTTGAACCCTGACCATCCCTGCTTCCGCCCTGACTCCACCAAAGTCGAGTCCTTGGTGGCCCTGCTCAAC AACTCTTCAGAGATGAAGCTAGTACAGATGAAGTGGCACGAGGCCTGCCTCAGCATTTCAGCAGCCATTT TGGAAATCCTCAACGCTTGGGAGAATGGGGTTCTGGCATTTGAGTCCATCCAGAAAATCACCGATAATAT CAAGGGAAAGGTATGCAGTCTGGCGGTGTGTGCTGTGGCTTGGCTCGTGGCCCACGTCCGGATGCTGGGG CTGGATGAGCGTGAGAAGTCACTGCAGATGATCCGCCAGTTGGCAGGGCCACTGTACAGCGAGAACACCC TGCAGTTCTACAATGAGAGGGTGGTGATTATGAACTCAATCTTGGAACACATGTGTGCGGACGTGCTCCA GCAGACAGCCACGCAGATCAAGTTTCCATCCACAGGCGTGGACACGATGCCCTACTGGAACCTGCTTCCT CCCAAGCGCCCCATCAAGGAGGTCCTGACAGACATCTTTGCCAAGGTACTGGAGAAGGGCTGGGTGGACA GCCGCTCCATCCACATCCTTGACACCCTGCTGCATATGGGCGGCGTCTACTGGTTCTGCAACAACCTGAT TAAGGAGCTGCTGAAGGAGACTCGCAAGGAGCACACGTTAAGGGCGGTCCAGCTTCTCTACTCCATCTTC TGTCTGGACATGCAGCAAGTGACCCTGGTCCTGCTGGGTCACATCCTGCCCGGGCTGCTTACTGACTCTT CCAAGTGGCACAGCCTTATGGACCCTCCTGGCACTGCGCTGGCCAAACTGGCTGTGTGGTGTGCCCTGAG CTCTTACTCTTCCCACAAGGGACAGGCGTCCTCCCGCCAGAAGAAGAGACACAGAGAGGACATTGAGGAC TATGTCAGCCTCTTCCCCGTGGAGGACATGCAGCCCTCGAAGCTGATGCGACTCCTGAGCTCCAGTGACG ATGATGCCAACATCCTGTCAAGTCCCACTGACCGCTCCATGAACAGCTCCCTCTCAGCCTCCCAGCTCCA CACAGTCAACATGAGGGACCCTCTGAACCGAGTCCTGGCCAACCTGTTCCTGCTCATTTCCTCCATCTTG GGTTCACGCACCGCTGGCCCCCACACCCAGTTTGTGCAGTGGTTCATGGAGGAGTGTGTGGGCTGCCTGG AGCAGGACAGCAGGGGCAGCATTCTGCAGTTCATGCCCTTCACTACCGTATCAGAACTGGTGAAAGTGTC TGCTATGTCCAGTCCCAAGGTTGTTCTGGCCATCACAGACCTCAGCCTGCCCCTGGGCCGGCAGGTGGCC GCCAAAGCCATTGCTGCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211298 representing NM_011869
Red=Cloning site Green=Tags(s) MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLAEALLEQAMIGPSPNPLILSYLKYAISS QMVSCSSVLTAISKFDDFSRDLCVQALLDIMDMFCDRLSCHGKAEECIGLCRALLSALHWLLRCTAASAE RLQEGLEAGTPAPGEKQLALCLQCLEKTLSSTKNRALLHIAKLEEASSWTAIEHSLLKLGEILANLSNPQ LRSQAERCGTLIRSIPSMLSVHSEQLHKTGFPTIHALILLEGTMNLTGEMQPLVEQLMMVKRMQHIPTPL FVLEIWKACFVGLIESPEGTQELKWTAFTYLKIPQVLVKLKKYFHGEKDFTEDVNCAFEFLLKLTPLLDK ADQRCNCDCTNFLLQECNKQGLLSEVNFASLVGKRTADRDPQLKSSENANIQPNPGLILRAEPTVTNILK TMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTHGSGESTKTASVRALL FDISFLMLCHVAQTYGSEVILSESSSGEEVPFFETWMQTCMPEEGKILNPDHPCFRPDSTKVESLVALLN NSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFESIQKITDNIKGKVCSLAVCAVAWLVAHVRMLG LDEREKSLQMIRQLAGPLYSENTLQFYNERVVIMNSILEHMCADVLQQTATQIKFPSTGVDTMPYWNLLP PKRPIKEVLTDIFAKVLEKGWVDSRSIHILDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVQLLYSIF CLDMQQVTLVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASSRQKKRHREDIED YVSLFPVEDMQPSKLMRLLSSSDDDANILSSPTDRSMNSSLSASQLHTVNMRDPLNRVLANLFLLISSIL GSRTAGPHTQFVQWFMEECVGCLEQDSRGSILQFMPFTTVSELVKVSAMSSPKVVLAITDLSLPLGRQVA AKAIAAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011869 |
| ORF Size | 2961 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011869.3 |
| RefSeq Size | 3427 bp |
| RefSeq ORF | 2964 bp |
| Locus ID | 23989 |
| UniProt ID | Q99K74 |
| MW | 110.4 kDa |
| Gene Summary | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene may form a submodule of the mediator complex that magnifies the effects of activators on the general transcription machinery. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222996 | Med24 (untagged) - Mouse mediator complex subunit 24 (Med24), (10ug) |
CNY 12,830.00 |
|
| MG211298 | Med24 (tGFP-tagged) - Mouse thyroid hormone receptor associated protein 4 (Thrap4) |
CNY 7,890.00 |
|
| MR211298L3 | Lenti ORF clone of Med24 (Myc-DDK-tagged) - Mouse mediator complex subunit 24 (Med24) |
CNY 9,030.00 |
|
| MR211298L4 | Lenti ORF clone of Med24 (mGFP-tagged) - Mouse mediator complex subunit 24 (Med24) |
CNY 9,030.00 |
