Srrm1 (BC094322) Mouse Tagged ORF Clone

CAT#: MR211276

  • TrueORF®

Srrm1 (Myc-DDK-tagged) - Mouse serine/arginine repetitive matrix 1 (cDNA clone MGC:106668 IMAGE:6401250)

ORF Plasmid: DDK tGFP



  "BC094322" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 10,392.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms POP101, Srm160
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211276 representing BC094322
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCGGGATTCTTCCGCGGAACAAGTGCGGAGCAGGATAATCGGTTCAGCAACAAACAGAAGAAAC
TCCTCAAGCAGCTGAAATTTGCAGAATGCTTAGAAAAAAAGGTGGACATGAGCAAAGTAAATTTGGAGGT
TATAAAGCCTTGGATAACCAAAAGAGTAACTGAGATCCTTGGATTCGAAGATGATGTTGTCATTGAGTTT
ATATTCAACCAGCTGGAAGTGAAGAACCCAGATTCCAAAATGATGCAAATCAACCTGACTGGGTTTTTGA
ATGGGAAGAATGCTAGAGAATTCATGGGAGAGCTGTGGCCCCTGCTGTTGAGTGCACAAGAAAATATCGC
CGGAATCCCTTCTGCTTTCCTAGAGTTGAAGAAGGAAGAGATAAAGCAGAGACAAATTGAACAAGAAAAA
TTGGCGTCTCTGAAAAAACAAGATGAAGATAAAGATAAGAGGGATAAGGAAGAAAAGGAAAGCAGCAGAG
AGAAGAGGGAGCGGTCTCGCAGCCCAAGAAGACGCAAATCCAGATCTCCTTCCCCTAGAAGACGATCTTC
CCCTGTCAGGAGAGAGAGAAAGCGCAGTCACTCTCGATCTCCCCGTCACAGAACCAAGAGCCGGAGCCCT
TCCCCTGCCCCAGAAAAGAAGGAGAAATCTCCAGAGCTCCCAGAGCCATCCGTGAGGATGAAGGACTCCT
CAGTACAGGAGGCCACCTCTACAAGTGACATCTTGAAAGCTCCCAAGCCTGAGCCTGTACCAGAGCCCAA
AGAACCGTCTCCAGAAAAAAATTCCAAAAAGGAAAAGGAAAAGACTCGACCAAGATCTCGGTCACGATCC
AAATCTCGGTCTCGGACCCGATCTCGATCACCTTCTCATACTAGACCAAGACGGAGACATAGATCCCGAT
CAAGATCATACTCTCCTAGAAGGCGGCCAAGCCCAAGACGACGACCATCTCCCCGAAGAAGAACTCCACC
AAGACGGATGCCTCCTCCACCAAGGCACCGGAGGAGTAGGTCTCCAGGGAGACGAAGGAGGCGTTCTTCC
GCATCCCTGTCTGGAAGTAGCTCGTCGTCCTCTTCATCTCGTTCCCGGTCACCACCAAAGAAACCTCCCA
AGAGGACATCCAGCCCTCCTCGGAAAACGCGTAGGTTATCACCTTCAGCAAGTCCTCCACGGCGAAGACA
CCGGCCGTCATCTCCAGCAACTCCGCCACCCAAAACTCGCCATTCCCCAACTCCCCAGCAGTCAAACCGT
ACAAGAAAAAGTCGTGTTTCTGTGTCTCCAGGAAGAACCTCCGGTAAAGTGACAAAACATAAAGGTACTG
AGAAAAGAGAGTCACCTTCTCCAGCACCCAAGCCTAGAAAAGTGGAGCTGTCTGAGTCTGAAGAAGACAA
AGGCAGCAAAATGGCTGCAGCTGATTCTGTGCAGCAGAGAAGACAATACCGACGGCAGAACCAGCAGTCT
TCATCTGACTCTGGCTCCTCTTCCACCTCAGAAGATGAGCGGCCCAAGAGATCCCATGTAAAGAACGGTG
AGGTAGGCAGGCGGCGGAGACATTCCCCTTCTCGGAGTGCCTCTCCATCACCTCGAAAGCGCCAGAAAGA
GACTTCCCCTCGGATGCAGATGGGAAAGCGATGGCAGTCACCAGTGACTAAAAGTAGTAGAAGGAGGAGA
AGTCCCTCTCCCCCTCCTGCCAGAAGGCGAAGGTCTCCTTCTCCAGCCCCTCCGCCGCCACCTCCCCCTC
CTCCTCCTCGGCGGCGCAGATCTCCCACCCCACCACCACGACGAAGGACCCCTTCTCCTCCCCCACGCCG
CCGCTCACCGTCTCCAAGAAGATACTCTCCTCCCATTCAGAGGAGATACTCTCCTTCCCCCCCTCCAAAG
AGGAGAACCGCCTCCCCCCCGCCCCCGCCCAAGCGAAGGGCATCACCATCTCCACCACCAAAGCGCCGGG
TCTCCCACTCTCCACCTCCTAAACAAAGAAGCCCCACAGTCACCAAGAGACGCTCGCCCTCCTTATCTTC
AAAACATAGGAAAGGGTCTTCCCCAGGCCGATCCACCCGGGAGGCCCGCTCACCACAACCAAACAAACGG
CATTCGCCCTCACCACGGCCTCGGGCTCCTCAGACCTCAAGCCCTCCCCCTGTACGAAGAGGAGCTTCAG
CGTCACCCCAAGGAAGGCAGTCCCCATCTCCAAGTACTAGGCCTATTAGGAGAGTCTCCAGGACCCCGGA
GCCCAAAAAGATAAAAAAGGCTGCCTCACCAAGTACTAGGCCTATTAGGAGAGTCTCCAGGACCCCGGAG
CCCAAAAAGATAAAAAAGGCTGCCTCACCAAGCCCTCAGTCCGTAAGAAGGGTTTCATCTTCCAGATCTG
TCTCTGGATCTCCTGAGCCAGCAGCTAAAAAGCCGCCAGCACCTCCCTCTCCTGTGCAGTCTCAGTCACC
CTCCACAAACTGGTCACCTGCAGTACCAGCCAAAAAGGCTAAGAGCCCAACACCAAGCCTGTCCCCTGCC
CGGAATTCTGATCAAGAAGGAGGTGGGAAGAAAAAGAAGAAGAAGAAGGACAAGAAACACAAGAAGGATA
AGAAACACAAGAAGCACAAAAAACACAAGAAGGAGAAGGCTGTGACCATAGCCACCCCAGCTACTGCAGC
CCCTGCAGCTGTTTCTGCTGCCACCACCACATCAGCACAGGAAGAGCCTGCAGCAGCACCAGAGCCCAGG
AAGGAGACTGAGAGTGAAGCTGAAGATGACAACCTTGATGACCTAGAGAGGCACCTGCGGGAGAAGGCCC
TGCGGTCCATGCGGAAGGCTCAAGTGTCCCCACAGTCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR211276 representing BC094322
Red=Cloning site Green=Tags(s)

MDAGFFRGTSAEQDNRFSNKQKKLLKQLKFAECLEKKVDMSKVNLEVIKPWITKRVTEILGFEDDVVIEF
IFNQLEVKNPDSKMMQINLTGFLNGKNAREFMGELWPLLLSAQENIAGIPSAFLELKKEEIKQRQIEQEK
LASLKKQDEDKDKRDKEEKESSREKRERSRSPRRRKSRSPSPRRRSSPVRRERKRSHSRSPRHRTKSRSP
SPAPEKKEKSPELPEPSVRMKDSSVQEATSTSDILKAPKPEPVPEPKEPSPEKNSKKEKEKTRPRSRSRS
KSRSRTRSRSPSHTRPRRRHRSRSRSYSPRRRPSPRRRPSPRRRTPPRRMPPPPRHRRSRSPGRRRRRSS
ASLSGSSSSSSSSRSRSPPKKPPKRTSSPPRKTRRLSPSASPPRRRHRPSSPATPPPKTRHSPTPQQSNR
TRKSRVSVSPGRTSGKVTKHKGTEKRESPSPAPKPRKVELSESEEDKGSKMAAADSVQQRRQYRRQNQQS
SSDSGSSSTSEDERPKRSHVKNGEVGRRRRHSPSRSASPSPRKRQKETSPRMQMGKRWQSPVTKSSRRRR
SPSPPPARRRRSPSPAPPPPPPPPPPRRRRSPTPPPRRRTPSPPPRRRSPSPRRYSPPIQRRYSPSPPPK
RRTASPPPPPKRRASPSPPPKRRVSHSPPPKQRSPTVTKRRSPSLSSKHRKGSSPGRSTREARSPQPNKR
HSPSPRPRAPQTSSPPPVRRGASASPQGRQSPSPSTRPIRRVSRTPEPKKIKKAASPSTRPIRRVSRTPE
PKKIKKAASPSPQSVRRVSSSRSVSGSPEPAAKKPPAPPSPVQSQSPSTNWSPAVPAKKAKSPTPSLSPA
RNSDQEGGGKKKKKKKDKKHKKDKKHKKHKKHKKEKAVTIATPATAAPAAVSAATTTSAQEEPAAAPEPR
KETESEAEDDNLDDLERHLREKALRSMRKAQVSPQS

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC094322
ORF Size 2838 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC094322.1
RefSeq Size 3112 bp
RefSeq ORF 2840 bp
Locus ID 51796
MW 114.1 kDa
Gene Summary Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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