Pcdha7 (BC080863) Mouse Tagged ORF Clone

CAT#: MR211274

  • TrueORF®

Pcdha7 (Myc-DDK-tagged) - Mouse protocadherin alpha 7 (cDNA clone MGC:90581 IMAGE:30356469)

ORF Plasmid: DDK tGFP



  "BC080863" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,928.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Cnr4; Crnr4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211274 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAAACCTGAGAGGATACAACTGGAAGAGCCAACAATTACTCCTGTTCCTGATAATTGTGGCAGCCT
GGGAGGCAGGGAGCGGCCAGCTCCACTACTCCGTCCCAGAGGAAGCCAAACACGGCACCTTCGTGGGCCG
CATCGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGGGTGGCGTCCAAGGAC
CGCGGGGACCTTCTGGAGGTAAATCTTCAGAATGGCATTTTGTTTGTGAATTCTCGCATCGACCGGGAGG
AACTGTGCGGGCGGAGCGCGGAGTGTAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTT
CCACGTGGAGGTGGAGGTGAAGGACATTAATGACAACCCTCCCATGTTTCCAGCGACACAAAAGGCTCTG
TTTATCCTGGAGTCCAGACTGCTTGACTCGCGGTTTCCACTAGAGGGCGCGTCAGATGCAGATGTCGGTT
CCAATGCTCTGCTGACTTACAGACTGAGCACCAATGAACATTTCTCTCTGGATGTACCGCCCAACCACGA
GCAGGTGAAACCTCTTGGGCTGGTTTTGCGGAAACCTTTAGACAGAGAAGAAGCTGCAGAGATTCGCTTA
TTGCTCACGGCCACCGACGGAGGCAAACCGGAGCTGACAGGCACCGTTCAATTACTCATCACCGTGCTGG
ATGTCAATGACAATGCTCCAGTTTTTGACAGATCTCTGTATACTGTGAAATTGCCAGAAAACGTTCCAAA
TGGAACATTGGTAATCAAAGTCAATGCCTCAGATTTGGACGAAGGCGTGAATGGGGACGTTATGTACTCA
TTTTCTAGTGATGTTTCTTCAGATATAAAATCCAAGTTCCACATGGACACTGTGAGTGGAGAGATTACAG
TTATAGGAATTATTGATTTCGAAGAGAGTAAAGCTTACAAGATTCCATTGGAGGCACGTGATAAAGGGTT
CCCACAACTGCCTGGACACTGTACCATTCTTGTGGAAGTTGTAGATGCTAATGACAACGCTCCACAGTTG
ACGGTTAGTTCCTTGTCTCTCCCAGTATCCGAGGACAGTCAACCTGGAAGAGTCGTCACTTTAATTAGCG
TATTTGACCGTGATTCCGGTGCCAATGGGCAGGTGACCTGCTCCCTGACTCCTCATATCCCCTTCAAGCT
GGTGTCCACATTCAAAAATTACTATTCGCTCGTGCTGGACAGTGCTCTGGACCGAGAGACCATCGCTAAC
TATGATGTGATTGTGACCGCTCGCGATGGCGGTTCGCCCTCCCTGTGGGCCACGGCCAGCGTGTCCGTGG
AGGTGGCTGATGTGAACGACAATGCTCCACTGTTTGCGCAGCCTGAATACACGGTGTTCGTGAAGGAGAA
CAACCCGCCTGGTGCGCACATCTTTACAGTGTCAGCGATGGATGCTGATGCGCAGGAGAATGCGCTGGTG
TCCTACTCGCTGGTGGAACGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTCTGTGCACGCGGAGA
GCGGCAAGGTGTTCGCTCTGCAGCCTCTGGACCATGAGGAGCTGGAACTGCTGCAGTTCCAGGTGAGCGC
GCGGGATGCTGGTGTGCCTGCCCTGGGCAGCAATGTGACTCTGCAGGTGTTTGTACTGGACGAGAACGAC
AATGCACCTACCCTGCTGGGGCCTTTGGCTAACGGGGTTGGTGGCACAATGAATGAGGTAGTATCAAGAG
CTTTGGTGCCTGGTCAGGTTGTGGCGAAAGTCCGGGCCGTAGATGAGGACTCTGGATACAATGCGTGGTT
GTCTTTTGAGCTGCAGCCAGTTGCGGGTGGAGTTCGTAGTCCCTTCCGTGTAGGCCTGTACACAGGAGAA
ATCAGCACGACACGTGCTCTGGAGGAGACAGATGCCTTGCGGCAGTGCTTGTTAGTGTTGGTGAAAGATC
ACGGAGAACCTTCTCTGACCGCCACAGCCACGGTGTTGCTTTCTCTGGTAGACAGCGGCCAAACACAGAA
GGTCTCATCTAAGGTGTCAGCTGGAGCCTCCAGAGTGGATCAGAGGCTGGTGGATGTCAACGTGTACCTG
ATCATCGCCATCTGCGCGGTGTCCAGCCTGTTGGTGCTCACGCTACTGCTGTACACAGCGCTGCGCTGTT
CGGCGACGCCCACCGATGGAGCCTGTGCTCCAGGGAAGCCCATGCTGGTGTGCTCCAGCGCGGTGGGGAG
CTGGTCATACTCGCAGCAGAGGCGACAGAGAGTGTGCTCTGGAGAAGGCCCACCCAAGACCGACCTCATG
GCCTTCAGCCCCAGCCTACCTCAGGGTCCCAGCTCTACAGATAACCCCCGGCAGCCCAACCCTGACTGGC
GCTACTCTGCCTCGCTAAGAGCAGGCATGCACAGCTCTGTGCACCTGGAGGAGGCTGGCATTCTACGGGC
TGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCTGAGGCAGGAGAG
GTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCA
AACAGTCCGGTCCCGGTGAGTTGCCAGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCG
GCAGGAGCCTGCTAACATCTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGATTTTATA
ACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAA
AAGAGAAAGGGAACAGCACGACGGACAACAGTGACCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211274 protein sequence
Red=Cloning site Green=Tags(s)

MVNLRGYNWKSQQLLLFLIIVAAWEAGSGQLHYSVPEEAKHGTFVGRIAQDLGLELTELVPRLFRVASKD
RGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVEVKDINDNPPMFPATQKAL
FILESRLLDSRFPLEGASDADVGSNALLTYRLSTNEHFSLDVPPNHEQVKPLGLVLRKPLDREEAAEIRL
LLTATDGGKPELTGTVQLLITVLDVNDNAPVFDRSLYTVKLPENVPNGTLVIKVNASDLDEGVNGDVMYS
FSSDVSSDIKSKFHMDTVSGEITVIGIIDFEESKAYKIPLEARDKGFPQLPGHCTILVEVVDANDNAPQL
TVSSLSLPVSEDSQPGRVVTLISVFDRDSGANGQVTCSLTPHIPFKLVSTFKNYYSLVLDSALDRETIAN
YDVIVTARDGGSPSLWATASVSVEVADVNDNAPLFAQPEYTVFVKENNPPGAHIFTVSAMDADAQENALV
SYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVSARDAGVPALGSNVTLQVFVLDEND
NAPTLLGPLANGVGGTMNEVVSRALVPGQVVAKVRAVDEDSGYNAWLSFELQPVAGGVRSPFRVGLYTGE
ISTTRALEETDALRQCLLVLVKDHGEPSLTATATVLLSLVDSGQTQKVSSKVSAGASRVDQRLVDVNVYL
IIAICAVSSLLVLTLLLYTALRCSATPTDGACAPGKPMLVCSSAVGSWSYSQQRRQRVCSGEGPPKTDLM
AFSPSLPQGPSSTDNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGE
VSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPANISIRQEPANNQIDKSDFI
TFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC080863
ORF Size 2838 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC080863, AAH80863
RefSeq Size 3882 bp
RefSeq ORF 2840 bp
Locus ID 12939
MW 102.3 kDa
Gene Summary Calcium-dependent cell-adhesion protein involved in cells self-recognition and non-self discrimination (PubMed:27161523). Thereby, it is involved in the establishment and maintenance of specific neuronal connections in the brain (PubMed:27161523).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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