United States
Germany
Japan
United Kingdom
China
Vps11 (NM_027889) Mouse Tagged Lenti ORF Clone
CAT＃: MR211256L4
- LentiORF®
Lenti ORF clone of Vps11 (mGFP-tagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11)
"NM_027889" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 1200011A11Rik |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211256).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_027889 |
| ORF Size | 2823 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027889.1 |
| RefSeq Size | 3194 bp |
| RefSeq ORF | 2826 bp |
| Locus ID | 71732 |
| UniProt ID | Q91W86 |
| Gene Summary | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes. Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205803 | Vps11 (untagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11), (10ug) |
CNY 6,896.00 |
|
| MG211256 | Vps11 (tGFP-tagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11) |
CNY 7,890.00 |
|
| MR211256 | Vps11 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11) |
CNY 6,896.00 |
|
| MR211256L3 | Lenti ORF clone of Vps11 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11) |
CNY 9,030.00 |
