1200016B10Rik (BC060204) Mouse Tagged ORF Clone

CAT#: MR211255

  • TrueORF®

1200016B10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1200016B10 gene (cDNA clone MGC:73488 IMAGE:6855442)

ORF Plasmid: DDK tGFP



  "BC060204" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 9,410.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1200016B10Rik; C1orf26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211255 representing BC060204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTTTGTACTACCAGTGCTCACAAGCTTGAGACTGTTTATGACCATAAGTTTCTAAAGATGTCTA
TCAAAGAATCTTGTGCAAAGGAAGAAAAGTCTCAGAAAAAACAGACTATCTCTTCACCAACTTTTAATGA
AGACAAGAAAAAGGGAGAAATCTCAGCAGGTTCTACTAGTTCAGAACATGGCGTTCAGCCTGTTTCAACC
AAGAAGAGAAAACTGAAAGCAGATGATACAGACAATGTATACTATAATGCAAACAGAAAAAACAGTAAAA
GATTGAATGTAGAAGTTTTCATTCCTAAAAAGAGACTAAAATTCAGTTCTTCGACCCAAGCGGTATCATA
TTTAAATAATAATCAAATGACTTCACACAGTTGTTCATCAAATGGAACTAAAGATACTAAAGTTAAAGAT
TGTAAATTGACAAATATTGGAAGTAAGCTTAATTATGAAATTAAAAACCATAGCCGCATTAAAATTACCA
AAGATATGAAATCTAAACCTGTAGACCAGACAAAGGAAAAGAATTGGCCTTCTTTGCTTATTCAGAAGAA
GATGAAAGAACTCAAGAAAGAAAAAAATAATAAAGACAGTTCTGAAGAATTGGAGAAATGCAAGAAAAAT
CACCTTCCTCAGAATTATAATTTTAGCAATATGATCAAGGAATCATTTGAATCAGGAAGAAAGAAGATTA
GCTTCAAAATTCCAAAAAAATCCTCTACTACCCTCCAGAAGCTTGTCGAAGAAAAGATCTTTACTATAGA
TTCTAGCAAGTCAAAGAGTAAGCAGGAGGAGAAGCAACACTTGCAAAGTCACCAGATGTCATTAAATCTG
GCCAGGCACAAAACTGAAAACTCATTTTCAGATTCTACACATAAGCAGTCTGTTTGTGAGAGGATAAGAA
AGGACCATTGTGAGCACCAAGGAACGAGTAGTTCCGATTCTAAGGAAAACCATGCCCAGAGTTTTGAAAC
ACCATGTTCTTCAGTGTCATCTGAGGATACCCAAGATGCAGATGAAGAGATGCAGATAGTAGAAGAGCTC
CACGCTGCACGTGTCGGGAGAAGCGTGGATCTGCCTGTGGTTCCACCGTCTGGAGAGCTCATGAGCATGG
AGATTGACCTGGTGGATGAGGACGCACATCCCTCTGCTGCACACACTGCCTCAGACACGAAGCTTCTCAT
TGTTATGGACACAAATGTTCTCATGAATCATCTCAAATTCGTTAAAATTTTGAAGACGACAGAAATACCA
GGATTTGACACACTTGTGTTAATAATTCCTTGGGTGGTTATACAAGAGCTAGATCGTATGAAGGCAGGAA
AATTACTAAAACATGTTCAGCATAAAGCTGTACCTGCAATTCATTTTATCAACAACAGCCTCAAAAGTCA
AGACAGAAAGCTGTGGGGCCAGTCATTACAGCTGGCATCCCAAAAACTTTATGGACTGAGTGACGAGAAC
AATGATGACCGAGTATTAAAGTGCTGTCTTCAGTACCAGCAATTATTCCCGTGTTCTCTTGTTATTCTGT
GCACGGATGATAGAAATTTAAGAACTAAAGGCCTGATAAGTGGTGTGAAGTCCCTCAGTAAAGAAGATCT
GGATACAGAGATATTAAATTTAACTCTGAACACAGATTTGTGTCATCAGCCTTGTATTTCTAAGCAGCAG
TTGAACGCAGAAGTACCACCTGCGAAAGAGACTCCTAAGGAAGGAGCTGCAAATTCCGGACTGTCCATTC
TACTGGAGCGGATCATTTGTGATCTTGAAAAATGCCTTGGGACAGCTTTATCTTCAATATTAGAAACAGA
AATGAAAATTGCTTTTGGAAATCTTTGGATGGAGGTGCTGTATTTGAAACCACCATGGACTCTAATAAAT
CTATTACAGTGCTTTAAAAAACATTGGCTGGCTGTATTTGGATTAGTTATGGAAAATAACTTGCTTGTAA
CTGTTGAGAGCCTATATGAAAATCTCTGTAAAGCAAATAAGGCAGTGGATTTTACAACAGTGAAATTCTT
GCTTCAGGATTCTAAAAGTTTGTTACATGCCTTCAGTACAAGGTCAAATTATGATGGAGTTCTTCCCCAG
ACCTTTGCTCAAGTAAACAAACTACTGCAAGTACTCGCAGAGGTCAAGACAAAACTGAAGCCAAGTTCTT
CAGAAAGCACAAGGAGTAAAGAGCAGGAAGACACTTCTTTGGTGAAGCTTCATAACCAAGATGTCAACGT
TTTCCTGGATTCTAATCTTCCCCAGGCCACCAGGCATCAGGAAATCTGGTCTGTCCTGGAGAGTGTTTGG
ATGTCAATATATCAGAACAGCATCTCTGTCTTCATAAGTGACACAGTAACTTTTAACCTGGTTGCTGTGT
CAATATATCAGAACAGCACAGATGTATTTCAAACACTGGACTCAAGTTCAACCCTGACAACTTCGAAAAT
AGCATCCTTTGAAGAAGCTTTTGTGTATCTTCAAAAGTTAATGGTGGCTGTGAAAGATATTCTTGAAGGA
ATTCAAAGGATTTTGGCTCCTAATAGTAATTACCAAGATGTTGAGACTCTCTATAACTTCCTAATCAAGT
ATGAGGTAAACAAAAATGTCAAATTCACTGCCCGGGAACTTTATGATTGTGTTTCTCAGACTGAGTACCG
GGAAAGATTAACCATTGGATGCCGCCAGCTGATTGAGATGGAATGCACCATGCAGCAGTGCAGTGCATCT
GTCTATATGGAGGCCAGAAGCAGAGGGTGGTGTGAAGACATGCTCAACAACAGGCTTAAGTTTACAGCAG
GAACAGCATCTCTGTCTTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211255 representing BC060204
Red=Cloning site Green=Tags(s)

MLLCTTSAHKLETVYDHKFLKMSIKESCAKEEKSQKKQTISSPTFNEDKKKGEISAGSTSSEHGVQPVST
KKRKLKADDTDNVYYNANRKNSKRLNVEVFIPKKRLKFSSSTQAVSYLNNNQMTSHSCSSNGTKDTKVKD
CKLTNIGSKLNYEIKNHSRIKITKDMKSKPVDQTKEKNWPSLLIQKKMKELKKEKNNKDSSEELEKCKKN
HLPQNYNFSNMIKESFESGRKKISFKIPKKSSTTLQKLVEEKIFTIDSSKSKSKQEEKQHLQSHQMSLNL
ARHKTENSFSDSTHKQSVCERIRKDHCEHQGTSSSDSKENHAQSFETPCSSVSSEDTQDADEEMQIVEEL
HAARVGRSVDLPVVPPSGELMSMEIDLVDEDAHPSAAHTASDTKLLIVMDTNVLMNHLKFVKILKTTEIP
GFDTLVLIIPWVVIQELDRMKAGKLLKHVQHKAVPAIHFINNSLKSQDRKLWGQSLQLASQKLYGLSDEN
NDDRVLKCCLQYQQLFPCSLVILCTDDRNLRTKGLISGVKSLSKEDLDTEILNLTLNTDLCHQPCISKQQ
LNAEVPPAKETPKEGAANSGLSILLERIICDLEKCLGTALSSILETEMKIAFGNLWMEVLYLKPPWTLIN
LLQCFKKHWLAVFGLVMENNLLVTVESLYENLCKANKAVDFTTVKFLLQDSKSLLHAFSTRSNYDGVLPQ
TFAQVNKLLQVLAEVKTKLKPSSSESTRSKEQEDTSLVKLHNQDVNVFLDSNLPQATRHQEIWSVLESVW
MSIYQNSISVFISDTVTFNLVAVSIYQNSTDVFQTLDSSSTLTTSKIASFEEAFVYLQKLMVAVKDILEG
IQRILAPNSNYQDVETLYNFLIKYEVNKNVKFTARELYDCVSQTEYRERLTIGCRQLIEMECTMQQCSAS
VYMEARSRGWCEDMLNNRLKFTAGTASLSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060204
ORF Size 2820 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060204.1
RefSeq Size 3008 bp
RefSeq ORF 2822 bp
Locus ID 66875
MW 110.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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