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Unc45b (NM_178680) Mouse Tagged ORF Clone
CAT＃: MR211221
- TrueORF®
Unc45b (Myc-DDK-tagged) - Mouse unc-45 homolog B (C. elegans) (Unc45b)
"NM_178680" in other vectors (3)
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CNY 6,808.00
CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA445617; Cmya4; D230041A13Rik; Unc45 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211221 representing NM_178680
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGCTGAAGCGGCACAGCTGAAGGAGGAGGGGAACCGGCATTTTCAGCTCCAGGATTACAAGG CGGCCACCAAGAGCTACAGCCAGGCCCTGAAGCTGACCAAGGACAAGGCTCTGCTAGCTACACTCTACCG GAATCGGGCAGCCTGCGGCCTGAAAATGGAGAGCTACGCCCAGGCAGCCTCGGATGCTTCCAGAGCCATT GACATCAACTCTGCTGACATCAAAGCATTGTATCGGCGATGCCAGGCACTGGAGCATCTTGGCAAGTTGG ACCAGGCATTCAAGGATGTGCAGCGCTGTGCTACCCTGGAGCCAAGGAACCAGAACTTCCAGGAGACACT GCGGAGGCTCAACACCAGCATCCAAGAGCAGCTCCGTGTGCAGTTTTCTACAGACTCCAGGGTACAGACG ATGTTTGAAATTCTTCTGAACGAAAACAGCGAGGCTGATAAGCGAGAGAAGGCTGCCAACAACCTCATTG TCCTGGGCCGTGAAGAAGCGGGTGCTGAGAGGATCTTCCAGAGCAATGGAGTGGCTCTGCTGCTGCAGCT TATGAACACCCAGCGGCCTGAGCTGCTGCTAGCGGCTGTGCGGACGCTGTCTGGCATGTGCAGTGGCCAC AGAGCTAGGGCCACCGCCATCCTGCACGCAGTCCGCATAGACAGAATCTGCAGCCTCATGGCCCTGGAGA ATGAAGAGATGTCCCTGGCTGTCTGCAACCTGCTCCAGGCCATCATTGACTCCCTGTCTGGAGAGGACAA GAGAGAGCATCGTGGGAAGGAGGAGGCCTTGGTTCTAGATACCAAGAAGGACCTGAAGCAGATCACCAGC CACCTGCTGGACATGCTGGTCAGTAAGAAAGTGTCTGGGCAGGGCAGAGACCAGGCACTGAACCTGCTTA ATAAGAATGTCCCCAGGAAGGACCTGTCCATTCATGACAACTCACGCACCATCTATGTTGTAGATAACGG TCTGAGGAAGATCCTGAAGGTGGTGGGGCAGGTTCCAGACCTGCCCTCCTGCCTACCCCTGACGGACAAC ACCCGCATGCTGGCCTCCATCCTCATCAACAAGCTCTATGATGACCTGCGCTGTGACCCAGAGCGCGACC ACTTCCGCAAGATCTGTGAGGAGTATATCACGAGCAAATTTGACCCCCAGGACATGGACAAGAATGTAAA TGCCATCCAGACGGTGTCCGGCATCTTGCAGGGGCCCTTTGACCTGGGCAATCAGCTGCTGGGCATGAAA GGTGTGATGGAGATGATGGTGGCTCTGTGTGGCTCGGAGCGCGAGGCAGACCAGCTGGTGGCTGTGGAGG CTCTCATCCACGCCTCCACGAAGCTCAGCCGGGCCACCTTCATCATCACCAACGGAGTGACACTTCTTAA GCAGATCTACAAGACCACAAAGAATGAGAAGATCAAGATCCGCACGCTGGTGGGGCTCTGTAAACTTGGC TCAGCTGGAGGCTCCGACTATGGTCTCAGACAGTTTGCTGAAGGGTCAACAGAGAAGCTAGCCAAGCAGT GTCGAAAGTGGCTGTGTAACACCGCCATAGACACCCGGACGCGGCGCTGGGCAGTGGAGGGCCTGGCCTA CCTCACGCTGGACGCTGACGTGAAGGACGACTTTGTCCAGGACATCCCTGCTCTGCAGGCCATGTTTGAG TTGGCCAAGACCAGTGACAAGACCATCCTCTACTCAGTGGCCAACACCCTGGTAAACTGCACCAACAGCT ATGATGTCAAGGAGGTCGTCCCTGAGCTGGTGCAGCTGGCCAAGTTCTCCAAGCAACATGTGCCAGAGGA GCACCCCAAGGACAAGAAGGACTTCGTTGACTTGCGGGTGAAACGTCTTCTGAAGGCAGGTGTCATATCT GCACTGGCCTGCATGGTGAAGGCAGACAGTGCCATCCTCACAGACCAGACCAAGGAGCTGCTGGCCAGGG TATTCCTGGCACTCTGTGACAATCCAAAGGACCGGGGCACCATTGTAGCTCAAGGTGGTGGCAAGGCCCT GATCCCCCTGGCTTTGGAGGGCACAGATGTGGGCAAGGTGAAGGCAGCCCATGGGCTAGCCAAGATTGCT GCTGTCTCCAACCCGGACATTGCCTTCCCTGGGGAGAGGGTATATGAGGTGGTGCGGCCCCTCGTGAGTC TCCTGGACACACAGAGGGATGGGCTTCAGAACTATGAGGCTCTGCTGGGACTGACTAACCTGTCTGGGCG CAGTGACAAACTCAGGCAGAAGATCTTCAAGGAGAAGGCCTTGCCAGACATTGAAAACTACATGTTTGAG AACCATGACCAGCTACGGCAGGCAGCCACCGAATGCATGTGTAACATGGTGCTCAATAAGGAGGTTCAGG AGAGGTTCCTGGCCGATGGGAATGATCGTCTGAAGCTGGTGGTGCTGCTCTGTGGGGAGGATGACCACAA ACTGCAGAACGCAGCAGCTGGAGCTCTAGCCATGCTGACCGCGGCACATAAGAAACTGTGCCTCAAGATG ACGGAGGTGACAACCCAGTGGCTGGAGATCCTACAGCGGCTTTGCCTGCATGACCAGTTGTCCGTGCAGC ACCGGGGCCTGGTCATTGCCCACAACCTGCTATCAGCGGATGCTGAGCTGGCGAGGAAGCTGGTGGAGAG CGAGCTGCTGGAGATCCTGACTGTGGTGGGAAAGCAGGAGCCAGATGAGAAGAGAGCGGCTGTGGTCCAG ACAGCCCGGGAGTGTCTTATCAAGTGCATGGATTATGGCTTCATCAAGCCGGTGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211221 representing NM_178680
Red=Cloning site Green=Tags(s) MAEAEAAQLKEEGNRHFQLQDYKAATKSYSQALKLTKDKALLATLYRNRAACGLKMESYAQAASDASRAI DINSADIKALYRRCQALEHLGKLDQAFKDVQRCATLEPRNQNFQETLRRLNTSIQEQLRVQFSTDSRVQT MFEILLNENSEADKREKAANNLIVLGREEAGAERIFQSNGVALLLQLMNTQRPELLLAAVRTLSGMCSGH RARATAILHAVRIDRICSLMALENEEMSLAVCNLLQAIIDSLSGEDKREHRGKEEALVLDTKKDLKQITS HLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLSIHDNSRTIYVVDNGLRKILKVVGQVPDLPSCLPLTDN TRMLASILINKLYDDLRCDPERDHFRKICEEYITSKFDPQDMDKNVNAIQTVSGILQGPFDLGNQLLGMK GVMEMMVALCGSEREADQLVAVEALIHASTKLSRATFIITNGVTLLKQIYKTTKNEKIKIRTLVGLCKLG SAGGSDYGLRQFAEGSTEKLAKQCRKWLCNTAIDTRTRRWAVEGLAYLTLDADVKDDFVQDIPALQAMFE LAKTSDKTILYSVANTLVNCTNSYDVKEVVPELVQLAKFSKQHVPEEHPKDKKDFVDLRVKRLLKAGVIS ALACMVKADSAILTDQTKELLARVFLALCDNPKDRGTIVAQGGGKALIPLALEGTDVGKVKAAHGLAKIA AVSNPDIAFPGERVYEVVRPLVSLLDTQRDGLQNYEALLGLTNLSGRSDKLRQKIFKEKALPDIENYMFE NHDQLRQAATECMCNMVLNKEVQERFLADGNDRLKLVVLLCGEDDHKLQNAAAGALAMLTAAHKKLCLKM TEVTTQWLEILQRLCLHDQLSVQHRGLVIAHNLLSADAELARKLVESELLEILTVVGKQEPDEKRAAVVQ TARECLIKCMDYGFIKPVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178680 |
| ORF Size | 2787 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178680.4, NP_848795.3 |
| RefSeq Size | 3677 bp |
| RefSeq ORF | 2790 bp |
| Locus ID | 217012 |
| UniProt ID | Q8CGY6 |
| MW | 103.4 kDa |
| Gene Summary | Acts as a co-chaperone for HSP90 and is required for proper folding of the myosin motor domain. Plays a role in sarcomere formation during muscle cell development (PubMed:12356907, PubMed:18326487, PubMed:18478096). Is necessary for normal early lens development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204882 | Unc45b (untagged) - Mouse unc-45 homolog B (C. elegans) (Unc45b), (10ug) |
CNY 6,808.00 |
|
| MR211221L3 | Lenti ORF clone of Unc45b (Myc-DDK-tagged) - Mouse unc-45 homolog B (C. elegans) (Unc45b) |
CNY 10,070.00 |
|
| MR211221L4 | Lenti ORF clone of Unc45b (mGFP-tagged) - Mouse unc-45 homolog B (C. elegans) (Unc45b) |
CNY 10,070.00 |
