Dgkz (NM_138306) Mouse Tagged ORF Clone
CAT#: MR211219
- TrueORF®
Dgkz (Myc-DDK-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 2
ORF Plasmid: tGFP
"NM_138306" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,310.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | E130307B02Rik; ENSMUSG00000075035; F730209L11Rik; mDGK[z] |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211219 representing NM_138306
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCGGGACCCCAGCCCCGAGGGCCGGAGCAGCGACTCGGAGTCGGCCTCCGCCTCGTCCAGCG GCTCCGAGCGCGACGCGGGTCCGGAGCCCGACAAGGCGCCGCGGCGCCTGACCAAGCGGCGCTTCCCGGG GCTGCGGCTCTTTGGGCACAGGAAAGCCATCACCAAGTCGGGCCTCCAGCACCTGGCACCCCCTCCTCCC ACGCCCGGGGCCCCGTGCGGTGAATCTGAGGAGCAGATCCAGAGCACTGTGGACTGGAGTGAGTCAGCAG TGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGTGACTTCTGCTATGTCGGGGAGCAGCACTG TGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAAAAAGTGTGCAGCCTGTAAGATCGTGGTGCATACC CAATGCATTAAGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCGTCCTTCCGCGAATCAGGCTCCAGGA ATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGACGACGCCAGGATGGCAAGTGTCGGCA CTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGATCGTAGCCATCAGCTGCTCCTGG TGCAAACAGGCATACCACAGCAAGGTGTCGTGCTTCATGATGCAACAGATTGAGGAACCCTGCTCCCTAG GGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTGCGGGCCCGGAGGCCCCAGAACACCCTCAA GGCCAGCAAGAAGAAAAAGAGAGCGTCCTTCAAGAGGAGGTCCAGCAAGAAAGGACCTGAGGAAGGCCGC TGGAGACCCTTCATCATCAGACCCACCCCATCCCCCCTCATGAAACCCCTGCTGGTGTTTGTGAACCCCA AGAGTGGGGGCAACCAGGGTGCCAAGATCATCCAGTCTTTTTTGTGGTATCTGAATCCCCGACAAGTCTT TGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAAAGTACATAATTTGAGGATTCTG GCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTCTCCACCCTGGACCAGCTGCGCTTAAAACCACCGC CTCCTGTAGCCATCCTGCCTCTGGGCACTGGCAATGACCTGGCCCGAACCCTCAACTGGGGTGGGGGTTA CACAGATGAGCCTGTGTCAAAGATCCTTTCCCATGTTGAGGAGGGGAATGTGGTACAGCTGGACCGTTGG GACCTCCGAGCAGAGCCCAACCCTGAGGCGGGGCCTGAGGAGCGAGATGATGGAGCCACTGACCGGCTGC CCCTGGATGTCTTTAACAACTACTTCAGCCTGGGCTTCGATGCCCACGTCACCCTGGAATTTCATGAGTC TCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGATGTTCTATGCTGGGACGGCCTTC TCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGAGTAGTGTGTGACGGAATGGACC TAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATCGTCTTTCTGAATATCCCCAGGTACTGTGCAGG CACCATGCCCTGGGGCCACCCCGGGGAGCACCATGACTTCGAGCCCCAGAGGCATGATGATGGCTACCTG GAGGTCATCGGCTTCACCATGACATCCTTGGCAGCACTGCAGGTGGGTGGGCACGGCGAGCGATTGACGC AGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGGTGGACGGTGAGCCCTGCAAGCT TTCAGCATCACGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGTGCAGAAGGCCAAGCGCCGGAGT ACTGCCCCACTGCACAGCGACCAGCAGCCAGTTCCTGAGCAGCTGAGGATCCAGGTGAGCAGGGTCAGTA TGCACGACTACGAGGCTCTACATTATGACAAGGAGCAGCTCAAGGAAGCCTCTGTGCCTCTGGGCACTGT GGTGGTCCCCGGAGACAGTGACCTGGAGCTCTGCCGCGCCCACATTGAGAGACTGCAGCGGGAGCCCGAT GGGGCTGGAGCCAAGTCCCCGATGTGCCACCAACTCTCCTCCAAGTGGTGTTTCCTGGATGCCACCACTG CCAGCCGCTTCTACAGGATCGACAGAGCCCAGGAGCACCTCAACTATGTGACGGAGATTGCCCAGGACGA GATTTACATCCTAGACCCAGAGCTTCTGGGAGCATCAGCCCGGCCTGACCTCCCCACCCCTACCTCCCCA CTCCCTGCTTCCCCCTGCTCCCCTACACCCGGGTCAATGCAGGGGGACACTGCACTGCCCCAAGGTGAAG AACTGATTGAAGCAGCCAAGAGGAATGACTGCTGCAAGCTCCAGGAGCTACACCGAGCAGGAGGTGACCT CATGCACCGTGACCAGAAGAGCCGCACGCTCTTGCACCACGCGGTCAGCACTGGCAGTAAGGAAGTGGTC CGCTATCTGCTGGATCATGCACCTCCAGAGATCCTTGATGCTGTGGAGGAGAATGGGGAGACCTGTCTAC ACCAGGCAGCCGCCCTGGGTCAGCGCACCATCTGCCACTACATTGTGGAAGCCGGGGCCTCCCTCATGAA GACAGATCTGCAGGGCGACACTCCCCGGCAGCGCGCTGAGAAGGCTCAAGACACAGAGCTCGCTGCCTAC CTGGAGAACAGACAGCATTACCAGATGATCCAGCGTGAGGACCAGGAGACAGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211219 representing NM_138306
Red=Cloning site Green=Tags(s) MEPRDPSPEGRSSDSESASASSSGSERDAGPEPDKAPRRLTKRRFPGLRLFGHRKAITKSGLQHLAPPPP TPGAPCGESEEQIQSTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCVAKMLPKSAPRKKCAACKIVVHT QCIKQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSW CKQAYHSKVSCFMMQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRRSSKKGPEEGR WRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRKVHNLRIL ACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRW DLRAEPNPEAGPEERDDGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAF SDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYL EVIGFTMTSLAALQVGGHGERLTQCREVLLTTAKAIPVQVDGEPCKLSASRIRIALRNQATMVQKAKRRS TAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQREPD GAGAKSPMCHQLSSKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSP LPASPCSPTPGSMQGDTALPQGEELIEAAKRNDCCKLQELHRAGGDLMHRDQKSRTLLHHAVSTGSKEVV RYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDLQGDTPRQRAEKAQDTELAAY LENRQHYQMIQREDQETAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_138306 |
ORF Size | 2787 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138306.2, NP_612179.2 |
RefSeq Size | 3499 bp |
RefSeq ORF | 2790 bp |
Locus ID | 104418 |
UniProt ID | Q80UP3 |
MW | 104.5 kDa |
Gene Summary | Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity (By similarity). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes. Activates PIP5K1A activity via generation of phosphatidic acid (PubMed:27739494).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222703 | Dgkz (untagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 2, (10ug) |
CNY 11,970.00 |
|
MG211219 | Dgkz (tGFP-tagged) - Mouse diacylglycerol kinase zeta (Dgkz) |
CNY 10,260.00 |
|
MR211219L3 | Lenti ORF clone of Dgkz (Myc-DDK-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 2 |
CNY 11,210.00 |
|
MR211219L4 | Lenti ORF clone of Dgkz (mGFP-tagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 2 |
CNY 11,210.00 |