Fuk (BC037698) Mouse Tagged ORF Clone
CAT#: MR211204
- TrueORF®
Fuk (Myc-DDK-tagged) - Mouse fucokinase (cDNA clone MGC:47125 IMAGE:4190449)
ORF Plasmid: tGFP
"BC037698" in other vectors (4)
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CNY 6,776.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110046B12Rik; AI303278 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211204 representing BC037698
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGAGACTTCCCCTTCGATGACTGTGGCAGGGCCTTCACTTGCCTCCCTGTGGAGAACCCACAGG CCCCTGTGGAGGCCTTGGTATGCAACCTGGACTGCCTGTTGGATATCATGACCCACCGGCTGGGTCCAGG TTCCCCACCAGGTGTGTGGGTCTGCAGCACCGACATGCTTCTGTCTGTTCCTCCAAACCCTGGGATCAGT TGGGATGGCTTCCGGGGAGCCAGAGTGATCGCCTTTCCTGGGAGCCTGGCCTATGCGTTGAACCACGGTG TCTACCTCACTGACTCACAGGGCTTGGTTTTGGACATTTACTACCAGGGCACTAAGGCGGAGATACAACG TTGTGTCGGACCTGATGGGCTGGTACCATTGGTCTCCGGGGTCGTCTTCTTCTCTGTGGAGACTGCTGAG CACCTCCTAGCCACCCATGTGAGCCCACCGCTGGATGCCTGCACCTATATGGGCTTGGACTCTGGAGCCC AGCCTGTGCAGCTGTCTCTGTTTTTCGACATCCTGCTCTGCATGGCTCGGAATATGAGCAGGGAGAACTT CCTGGCTGGGCGGCCCCCGGAGTTGGGGCAAGGTGACATGGATGTAGCAAGTTACCTGAAGGGAGCCCGG GCCCAGCTGTGGAGGGAGCTTCGAGATCAGCCCCTCACAATGGTGTATGTCCCTGACGGCGGCTACAGCT ACATGACGACTGATGCCACCGAGTTCCTGCACAGACTCACGATGCCTGGAGTAGCTGTGGCACAGATTGT TCACTCCCAGGTGGAGGAGCCACAGCTGCTAGAGGCTACGTGCTCGGTGGTCAGCTGCCTGCTCGAGGGC CCTGTGCACCTGGGGCCTCGAAGTGTCCTGCAGCACTGTCACCTGAGGGGCCCCATTCGCATCGGCGCTG GCTGCTTTGTGAGTGGTCTGGATACAGCCCACTCGGAGGCACTGCATGGCCTGGAGCTCCATGATGTCAT CCTGCAGGGACACCATGTGCGGCTGCATGGCTCCCTGAGCCGTGTATTTACTCTTGCTGGCCGTCTGGAC AGCTGGGAAAGACAGGGGGCAGGCATGTATCTCAACATGTCCTGGAATGAGTTCTTCAAGAAGACAGGCA TTCGAGACTGGGACCTGTGGGACCCAGATACACCCCCCTCAGATCGATGCCTCCTCACTGCCCGCCTTTT CCCTGTGCTCCACCCCACGAGGGCCCTGGGGCCCCAGGATGTGCTGTGGATGCTGCACCCCCGCAAACAC AGAGGTGAGGCCCTTCGGGCCTGGCGAGCCTCCTGGCGTCTGTCCTGGGAGCAGCTGCAACCTTGTGTGG ACCGGGCTGCCACACTGGACTTCCGCCGAGATCTGTTCTTCTGCCAGGCCTTGCAGAAGGCAAGGCATGT GTTAGAGGCGCGGCAGGACCTCTGCCTACGTCCACTGATCCGGGCCGCTGTCGGGGAAGGTTGCTCTGGG CCCCTGCTGGCCACACTTGACAAGGTTGCAGCTGGGGCAGAAGATCCTGGCGTGGCAGCCCGGGCTCTGG CTTGTGTGGCCGATGTGCTGGGCTGCATGGCAGAGGGCCGAGGAGGCTTGCGCAGTGGGCCAGCTGCCAA CCCTGAGTGGATTCAGCCTTTCTCATACTTGGAGTGTGGAGACCTGATGAGGGGTGTGGAGGCGCTTGCC CAGGAGAGAGAGAAGTGGCTGACCAGGCCTGCCTTGCTGGTTCGAGCTGCCCGCCATTACGAGGGGGCCG AGCAGATCCTGATCCGCCAGGCTGTGATGACAGCCCGGCACTTCGTCTCCACCCAGCCCGTGGAGCTGCC CGCACCCGGGCAGTGGGTGGTGACTGAGTGCCCAGCCCGTGTGGATTTCTCTGGGGGCTGGAGTGACACA CCGCCCATTGCCTATGAGCTTGGTGGAGCAGTGTTGGGCCTGGCTGTGCGGGTGGATGGCCGCCGGCCCA TCGGGGCCAAAGCACGCCGCATCCCGGAGCCTGAGCTCTGGCTGGCAGTGGGACCTCGGCAGGATGAGAT GACCATGAGGATAGTGTGCCGGAGCCTGGATGACCTGCGGGATTACTGCCAGCCTCATGCCCCAGGGGCC TTGCTGAAGGCAGCCTTTATCTGTGCTGGCATTGTGCATCTCCACTCAGAGCTCCCTCTGCTTGAACAGT TGTTACACTCCTTTAATGGTGGCTTTGAGCTGCACACGTGGTCAGAGCTGCCGCACGGCTCTGGTCTTGG CACCAGCAGCATCCTGGCAGGGGCTGCCCTGGCTGCCTTACAGCGGGCTGCAGGCCGGGCAGTGGGCACG GAGGCTCTCATCCACGCAGTGCTGCACCTGGAGCAGGTGCTCACCACAGGAGGTGGCTGGCAGGACCAAG TCAGTGGCCTAATGCCTGGCATCAAAGTGGGGCGCTCCCGGGCCCAGCTGCCCCTCAAGGTGGAGGTGGA GGAAATCACTGTGCCTGAGGGCTTTGTCCAGAAGAAGCTTATGGCCCCAGGCTGCGAGCCGCTGGCCGTG CAGCGAATGATGGATGTCCTGGCCCCGTATGCGTATGGCCAAAGCCTGGCAGGGGCAGGTGGTGGGGGCT TTCTCTATCTATTGACCAAGGAACCCCGGCAGAAAGAGACTCTGGAAGCTGTCCTGGCCAAGGCTGAGGG CCTTGGCAACTACAGTGTCCACCTGGTGGAAGTGGATCCTCAGGGCCTGAGCCTGCAGCTGCTGGGACAC GACACCCGTCTTTGTGGGGCCGGGCCCTCTGAAGTGGGCACCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211204 representing BC037698
Red=Cloning site Green=Tags(s) MGRDFPFDDCGRAFTCLPVENPQAPVEALVCNLDCLLDIMTHRLGPGSPPGVWVCSTDMLLSVPPNPGIS WDGFRGARVIAFPGSLAYALNHGVYLTDSQGLVLDIYYQGTKAEIQRCVGPDGLVPLVSGVVFFSVETAE HLLATHVSPPLDACTYMGLDSGAQPVQLSLFFDILLCMARNMSRENFLAGRPPELGQGDMDVASYLKGAR AQLWRELRDQPLTMVYVPDGGYSYMTTDATEFLHRLTMPGVAVAQIVHSQVEEPQLLEATCSVVSCLLEG PVHLGPRSVLQHCHLRGPIRIGAGCFVSGLDTAHSEALHGLELHDVILQGHHVRLHGSLSRVFTLAGRLD SWERQGAGMYLNMSWNEFFKKTGIRDWDLWDPDTPPSDRCLLTARLFPVLHPTRALGPQDVLWMLHPRKH RGEALRAWRASWRLSWEQLQPCVDRAATLDFRRDLFFCQALQKARHVLEARQDLCLRPLIRAAVGEGCSG PLLATLDKVAAGAEDPGVAARALACVADVLGCMAEGRGGLRSGPAANPEWIQPFSYLECGDLMRGVEALA QEREKWLTRPALLVRAARHYEGAEQILIRQAVMTARHFVSTQPVELPAPGQWVVTECPARVDFSGGWSDT PPIAYELGGAVLGLAVRVDGRRPIGAKARRIPEPELWLAVGPRQDEMTMRIVCRSLDDLRDYCQPHAPGA LLKAAFICAGIVHLHSELPLLEQLLHSFNGGFELHTWSELPHGSGLGTSSILAGAALAALQRAAGRAVGT EALIHAVLHLEQVLTTGGGWQDQVSGLMPGIKVGRSRAQLPLKVEVEEITVPEGFVQKKLMAPGCEPLAV QRMMDVLAPYAYGQSLAGAGGGGFLYLLTKEPRQKETLEAVLAKAEGLGNYSVHLVEVDPQGLSLQLLGH DTRLCGAGPSEVGTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC037698 |
ORF Size | 2775 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC037698.1 |
RefSeq Size | 3626 bp |
RefSeq ORF | 2777 bp |
Locus ID | 234730 |
MW | 132.9 kDa |
Gene Summary | Takes part in the salvage pathway for reutilization of fucose from the degradation of oligosaccharides.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220919 | Fuk (untagged) - Mouse fucokinase (cDNA clone MGC:47125 IMAGE:4190449), (10ug) |
CNY 9,120.00 |
|
MG211204 | Fuk (tGFP-tagged) - Mouse fucokinase (cDNA clone MGC:47125 IMAGE:4190449) |
CNY 5,890.00 |
|
MR211204L3 | Lenti ORF clone of Fuk (Myc-DDK-tagged) - Mouse fucokinase (cDNA clone MGC:47125 IMAGE:4190449) |
CNY 7,220.00 |
|
MR211204L4 | Lenti ORF clone of Fuk (mGFP-tagged) - Mouse fucokinase (cDNA clone MGC:47125 IMAGE:4190449) |
CNY 7,220.00 |