Usp20 (NM_028846) Mouse Tagged Lenti ORF Clone
CAT#: MR211168L4
- LentiORF®
Lenti ORF clone of Usp20 (mGFP-tagged) - Mouse ubiquitin specific peptidase 20 (Usp20)
"NM_028846" in other vectors (4)
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CNY 9,030.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 1700055M05Rik; AI467231; Vdu2 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR211168).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028846 |
ORF Size | 2748 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028846.5, NP_083122.1 |
RefSeq Size | 4157 bp |
RefSeq ORF | 2751 bp |
Locus ID | 74270 |
UniProt ID | Q8C6M1 |
Gene Summary | Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222595 | Usp20 (untagged) - Mouse ubiquitin specific peptidase 20 (Usp20), (10ug) |
CNY 11,780.00 |
|
MG211168 | Usp20 (tGFP-tagged) - Mouse ubiquitin specific peptidase 20 (Usp20) |
CNY 7,890.00 |
|
MR211168 | Usp20 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 20 (Usp20) |
CNY 6,712.00 |
|
MR211168L3 | Lenti ORF clone of Usp20 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 20 (Usp20) |
CNY 9,030.00 |