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Ercc4 (BC026792) Mouse Tagged ORF Clone
CAT＃: MR211135
- TrueORF®
Ercc4 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522
ORF Plasmid: tGFP
"BC026792" in other vectors (4)
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CNY 6,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI606920; Xpf |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211135 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGCTGTTGGAGTACGAGAGGCAGCAGGTGCTGGAACTGCTCGACAGCGACGGGCTGGTGGTGT GTGCCCGCGGACTCGGCACCGACCGGCTCCTCTACCATTTCCTGCGGCTTCACTGCCACCCGGCCTGCCT GGTGCTGGTGCTCAACACGCAGCCGGCCGAGGAGGAATATTTTATCAATCAGTTGAAGATAGAAGGAGTT GAACATCTCCCTCGACGAGTGACAAATGAAATCGCAAGTAACAGTCGCTATGAAGTCTACACGCAGGGTG GTATTATATTTGCAACAAGCCGAATACTCGTGGTTGATTTCTTGACAGGTAGAATACCTTCAGATTTAAT AACTGGCATCCTCGTGTACAGGGCACACAGAATCATTGAGTCCTGCCAGGAAGCCTTCATCCTGCGCCTC TTCCGCCAGAAAAACAAGCGCGGGTTCATCAAAGCTTTCACCGACAACGCTGTTGCCTTTGACACTGGCT TTTGTCACGTGGAAAGAGTGATGCGGAACCTTTTTGTGAGGAAGCTCTACCTGTGGCCAAGGTTCCATGT AGCAGTCAACTCATTCTTGGAACAACACAAGCCTGAAGTTGTAGAGATTCACGTGTCCATGACTCCCGCC ATGCTTGCCATTCAGACGGCCATACTAGACATCTTAAATGCATGTCTGAAAGAACTGAAATGTCACAACC CGTCACTTGAAGTAGAAGATTTGTCACTAGAAAACGCTCTTGGGAAGCCATTCGACAAGACAATCCGCCA TTACTTGGACCCTTTGTGGCATCAGCTTGGAGCCAAGACTAAATCCTTGGTGCAGGATTTAAAGATACTA AGAACCTTACTGCAGTATCTCTCTCAGTATGATTGTGTTACATTTCTGAATCTTCTGGAATCTCTGAGAG CAACAGAGAAGGTGTTTGGTCAGAATTCAGGTTGGCTTTTCCTGGATGCTAGCACCTCCATGTTTGTGAA TGCTCGTGCCCGAGTTTACCGAGTTCCAGATGTCAAACTGAACAAAAAGGCCAAAACATCAGAAAAGACG TCGAGTCCAGAAGTTCAAGAAACAAAAAAAGAACTGGTTCTAGAGAGCAATCCAAAGTGGGAGGCGCTGA CTGACGTCCTGAAAGAAATCGAAGCAGAAAATAAGGAGAGCGAAGCCCTTGGTGGCCCAGGCCGGGTGCT GATCTGTGCAAGCGATGACCGCACGTGCTGCCAGCTGAGAGACTACCTGTCCGCCGGAGCAGAAACCTTC CTGCTGCGACTTTATAGGAAAACTTTTGAGAAGGATGGCAAAGCTGAAGAAGTGTGGGTAAATGTTAGAA AGGGGGACGGCCCGAAGAGAACTACAAAATCTGACAAAAGACCAAAAGCCGCTCCAAACAAGGAGCGTGC TTCCGCCAAAAGGGGGGCCCCTCTCAAAAGGAAGAAGCAAGAGCTGACTCTGACGCAAGTTTTGGGGTCC GCTGAGGAGCCGCCGGAGGACAAGGCTTTGGAGGAAGACCTGTGCAGACAGACCAGTAGTAGCCCAGAAG GCTGCGGGGTAGAGATTAAGCGTGAATCATTTGATTTAAATGTGTCGTCTGATGCGGCCTATGGCATCCT GAAAGAGCCCCTGACCATCATCCATCCGCTTCTAGGGTGCAGCGACCCCTATGCCCTGACGCGGGTACTG CACGAGGTGGAGCCCAGATACGTGGTTCTGTATGATGCAGAGCTCACCTTTGTTCGCCAACTGGAGATCT ACAGGGCTAGCAGGCCTGGGAAACCTCTGAGGGTTTACTTTCTTATATACGGAGGCTCCACGGAAGAGCA GCGCTACCTGACTGCTCTGCGGAAAGAAAAGGAAGCCTTTGAAAAGCTCATAAGAGAAAAAGCTAGCATG GTTGTCCCCGAAGAACGGGAAGGCAGAGATGAGACCAACCTGGACCTAGCAAGAGGCTCAGCAGCCCTGG ATGCTCCCACGGACACTCGGAAAGCCGGTGGCCAGGAGCAGAATGGCACCCAGTCCAGCATCGTGGTGGA CATGCGTGAGTTTCGGAGCGAGCTCCCATCTCTGATCCATCGGCGGGGTATTGACATAGAGCCAGTGACT CTGGAGGTGGGTGATTACATCCTGACACCTGAGCTGTGTGTGGAGCGCAAAAGTGTGAGCGACCTCATTG GCTCGCTGCACAGTGGCCGCCTGTACAGCCAGTGCCTAGCCATGTCTCGCTACTACCGGAGGCCTGTGCT GCTCATTGAGTTTGACCCGAGCAAGCCCTTCTCCCTGGCGCCACGAGGTGCCTTCTTCCAGGAGATGAGC AGCAGCGATGTGAGCTCCAAGCTTACGCTCCTCACCTTGCACTTCCCGCGTTTGCGGCTGCTCTGGTGCC CCTCACCCCACGCCACAGCCGAGCTGTTTGAGGAGCTGAAGCAGAACAAGCCACAGCCTGATGCGGCCAC AGCCATGGCCATCACCGCAGACTCAGAGACACTGCCTGAGTCGGACAGATATAACCCCGGCCCCCAAGAC TTTGTGCTGAAGATGCCTGGGGTCAACGCCAAGAACTGTCGCTCCCTGATGAACCAAGTGAAGAACATCG CAGAGCTGGCCACCCTGTCCCTGGAACGACTCACAACCATCCTGGGGCACTCGGGAAATGCCAAGCAGCT GCACGACTTCCTGCACACTGCCTATGCAGACTTGGTGTCCAAAGGCAGAGTGAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211135 protein sequence
Red=Cloning site Green=Tags(s) MAPLLEYERQQVLELLDSDGLVVCARGLGTDRLLYHFLRLHCHPACLVLVLNTQPAEEEYFINQLKIEGV EHLPRRVTNEIASNSRYEVYTQGGIIFATSRILVVDFLTGRIPSDLITGILVYRAHRIIESCQEAFILRL FRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPEVVEIHVSMTPA MLAIQTAILDILNACLKELKCHNPSLEVEDLSLENALGKPFDKTIRHYLDPLWHQLGAKTKSLVQDLKIL RTLLQYLSQYDCVTFLNLLESLRATEKVFGQNSGWLFLDASTSMFVNARARVYRVPDVKLNKKAKTSEKT SSPEVQETKKELVLESNPKWEALTDVLKEIEAENKESEALGGPGRVLICASDDRTCCQLRDYLSAGAETF LLRLYRKTFEKDGKAEEVWVNVRKGDGPKRTTKSDKRPKAAPNKERASAKRGAPLKRKKQELTLTQVLGS AEEPPEDKALEEDLCRQTSSSPEGCGVEIKRESFDLNVSSDAAYGILKEPLTIIHPLLGCSDPYALTRVL HEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASM VVPEEREGRDETNLDLARGSAALDAPTDTRKAGGQEQNGTQSSIVVDMREFRSELPSLIHRRGIDIEPVT LEVGDYILTPELCVERKSVSDLIGSLHSGRLYSQCLAMSRYYRRPVLLIEFDPSKPFSLAPRGAFFQEMS SSDVSSKLTLLTLHFPRLRLLWCPSPHATAELFEELKQNKPQPDAATAMAITADSETLPESDRYNPGPQD FVLKMPGVNAKNCRSLMNQVKNIAELATLSLERLTTILGHSGNAKQLHDFLHTAYADLVSKGRVRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026792 |
| ORF Size | 2718 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026792, AAH26792 |
| RefSeq Size | 3235 bp |
| RefSeq ORF | 2720 bp |
| Locus ID | 50505 |
| MW | 102.5 kDa |
| Gene Summary | Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220780 | Ercc4 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522, (10ug) |
CNY 9,120.00 |
|
| MG211135 | Ercc4 (tGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522 |
CNY 5,610.00 |
|
| MR211135L3 | Lenti ORF clone of Ercc4 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522 |
CNY 7,030.00 |
|
| MR211135L4 | Lenti ORF clone of Ercc4 (mGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522 |
CNY 9,032.00 |
