Mtmr4 (BC058364) Mouse Tagged ORF Clone

CAT#: MR211115

  • TrueORF®

Mtmr4 (Myc-DDK-tagged) - Mouse myotubularin related protein 4 (cDNA clone MGC:66741 IMAGE:6820754)

ORF Plasmid: DDK tGFP



  "BC058364" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 6,608.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AA596759; ESTM44; FYVE-DSP2; mKIAA0647; ZFYVE11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211115 representing BC058364
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGAGGAGGGCCCCCCCAGCCTGGAGTACATCCAAGCCAAGGATCTGTTCCCCCCCAAGGAACTGG
TGAAGGAGGAGGAGAATCTTCAGGTACCCTTCATATTGCTGCAGGGTGAGGGAGTGGAATTCCTGGGCCG
GGCAACCGATGCCCTCATTGCCATCTCTAACTACCGGCTGCACATCAAGTTCAAAGACTCTGTCATCAAC
GTGCCCCTCCGGATGATTGACAGTGTGGAAAGCCGTGATATGTTTCAGTTGCACATTGCCTGCAAGGACT
CCAAAGTGGTGAGGTGCCACTTCTCTACTTTCAAGCAGTGCCAAGAGTGGCTCTCAAGGCTAAGCCGGGC
CACAGCGAGACCTGCCAAGCCCGAGGATCTCTTTGCCTTTGCCTACCATGCGTGGTGCCTGGGGCTGACC
GAGGAGGACCAGCATACCCACCTGTGCCAGCCAGGAGAGCACATCCGATGTCGACAGGAGGCAGAGCTCG
CAAGGATGGGTTTTGACCTGCAGAATGTCTGGAGAGTCTCGCATATCAACAGCAATTACAAGCTGTGCCC
TAGTTACCCCCAGAAGCTGCTGGTTCCTGTGTGGATCACAGATAAAGAGCTGGAGAACGTGGCTTCTTTC
CGCTCTTGGAAACGCATCCCTGTTGTGGTATACAGACACCTGCGCAACGGGGCTGCCATTGCTCGCTGCA
GCCAGCCTGAGATCAGCTGGTGGGGCTGGCGCAATGCTGATGATGAATACCTCGTCACGTCCATTGCCAA
AGCCTGCGCCTTAGACCCAGGGACAAGGGCCAGTGGGGGCTCCCTCAGCACTGGGACTAATGATGCCAGT
GAGGCATGTGACACAGATTTCGATTCTTCTCTGACTGCATGCTCTGGGGTGGAGAGCACAGCAGCCCCAC
AGAAACTGCTGATCCTGGATGCACGGTCCTACACAGCAGCCGTGGCTAACCGGGCCAAGGGTGGAGGCTG
TGAATGTGAAGAATACTATCCGAACTGTGAGGTCTTGTTTATGGGAATGGCCAACATCCACGCCATCCGG
AATAGCTTCCAGTACCTCCGGGCTGTGTGTAGCCAGATGCCAGATCCCAGCAACTGGTTGTCGGCACTGG
AGAGTACCAAATGGCTGCAGCACTTGTCAGTGATGCTAAAAGCAGCTGTGTTGGTAGCCAATACGGTAGA
TCGGGAAGGCCGGCCTGTGCTGGTACACTGCTCTGACGGCTGGGACCGTACACCACAGATTGTAGCCCTG
GCCAAAATACTACTGGATCCATATTACAGGACTCTGGAGGGCTTCCAAGTGTTAGTGGAGTCTGATTGGC
TGGATTTCGGACACAAGTTTGGAGACCGCTGTGGCCATCAGGAAAATGCAGAGGACCAAAATGAACAGTG
TCCTGTGTTCCTCCAGTGGCTTGATTCCGTGCATCAGCTGCTTAAGCAGTTCCCCTGCCTGTTTGAGTTC
AATGAAGCATTTCTGGTCCTGCATCCTGTGTGTCATGTCCGGGCCCTGCACCTCTGGACAGCTGTTTATC
TGCCTGCGTCATCTCCATGCACCCTTGGAGAGGAAAATATGGATCTTTACCTTTCCCCAGTGGCTCAGAG
CCAGGAATTTTCTGGTCGTTCTCTGGACAGGTTACCTAAAACCAGATCCATGGATGATCTGCTCTCTGCC
TGTGATACAAGCAGCCCCCTCACTCGTACTTCCAGCGACCCTAACCTGAATAACCACTCTCAGGAGGTCA
GAGGCAGCCTGGAGCCCTGGCACAGCAGTCCTGAAGGAGCAGAGACAGTCATAGACTCTGGAGTTGGAAG
CCCACAGCTGACTGTGGGAGAAATGGGCCTTCCTCCTCCTCTGCCCAGTAGCCAGAAAGAGTACTTGAGC
AATAAACCTTTCAAGGGTCATAAAAGTTGTTCTCTTAGTTATAAGTTGCTTAATACTTCTGTGTCCTGGG
AAATGAAGAGCAACACCTCTGACATCAAGGTCCTGGAAGAAACTGAAGCACTGGCCCCGGACCCTTCAGC
CCAAGAGGAGCAGGGTAGGACTTCTGATGGCTTAGGGAAGCCACCTGAACAGTTTCTAGAGAAGGAAGCT
GTCAGCTCACTCTGCAGTGTCTCTAGCAAGTGTGGTGGAGCCTGTGATTTTCCTGAGCCTCCCCAGGACC
CCCTAACAGGTACTCCCCAACAGCCCCACCTAGACTCCATGCAGATCTCGCCCTCAAGGTGCACTCCAGA
TCACAGTCAGGGCAGCCTTTGCAACCCACCAAGTGTGGCCAGCCAAACTCCAGAGCCAAATACTGACCTC
CTCAGCCAAGATCCCCCTGGTTCTACGGCAAGTATCTCCCACCAGGAACAGCCTAGCTCTGTGCCGGATC
TGATCTACAAGAAGGAGGATGCTGGCAAGAGAGGAAGTAAGAATGGGCAGCTGTTGGAAAATCCTCGCTT
TGGAAAAATGCCATTGGAGTTGGCCCGAAAGCCAATTTCTCAGAGCCAGATCAGTGAGTTCTCATTTTTA
GGGTCAAACTGGGACAGCTTCCAAGGGATGATGACTTCATTCCCAAGCGGAGAGACCACTCCTCGGCGCC
TGCTTGCTTATGGCTGTTGTAGCAAGAGGCCGAGCAATAAGCATATTCGAGCTGCAGGGCCCTGCCTTGG
GGGGCCAGTGGGCTCAGAGAGAAGGGATGAAATCTCCTGTCTGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211115 representing BC058364
Red=Cloning site Green=Tags(s)

MGEEGPPSLEYIQAKDLFPPKELVKEEENLQVPFILLQGEGVEFLGRATDALIAISNYRLHIKFKDSVIN
VPLRMIDSVESRDMFQLHIACKDSKVVRCHFSTFKQCQEWLSRLSRATARPAKPEDLFAFAYHAWCLGLT
EEDQHTHLCQPGEHIRCRQEAELARMGFDLQNVWRVSHINSNYKLCPSYPQKLLVPVWITDKELENVASF
RSWKRIPVVVYRHLRNGAAIARCSQPEISWWGWRNADDEYLVTSIAKACALDPGTRASGGSLSTGTNDAS
EACDTDFDSSLTACSGVESTAAPQKLLILDARSYTAAVANRAKGGGCECEEYYPNCEVLFMGMANIHAIR
NSFQYLRAVCSQMPDPSNWLSALESTKWLQHLSVMLKAAVLVANTVDREGRPVLVHCSDGWDRTPQIVAL
AKILLDPYYRTLEGFQVLVESDWLDFGHKFGDRCGHQENAEDQNEQCPVFLQWLDSVHQLLKQFPCLFEF
NEAFLVLHPVCHVRALHLWTAVYLPASSPCTLGEENMDLYLSPVAQSQEFSGRSLDRLPKTRSMDDLLSA
CDTSSPLTRTSSDPNLNNHSQEVRGSLEPWHSSPEGAETVIDSGVGSPQLTVGEMGLPPPLPSSQKEYLS
NKPFKGHKSCSLSYKLLNTSVSWEMKSNTSDIKVLEETEALAPDPSAQEEQGRTSDGLGKPPEQFLEKEA
VSSLCSVSSKCGGACDFPEPPQDPLTGTPQQPHLDSMQISPSRCTPDHSQGSLCNPPSVASQTPEPNTDL
LSQDPPGSTASISHQEQPSSVPDLIYKKEDAGKRGSKNGQLLENPRFGKMPLELARKPISQSQISEFSFL
GSNWDSFQGMMTSFPSGETTPRRLLAYGCCSKRPSNKHIRAAGPCLGGPVGSERRDEISCLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058364
ORF Size 2706 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058364.1
RefSeq Size 4452 bp
RefSeq ORF 2708 bp
Locus ID 170749
MW 163.2 kDa
Gene Summary Dephosphorylates proteins phosphorylated on Ser, Thr, and Tyr residues and low molecular weight phosphatase substrate para-nitrophenylphosphate. Phosphorylates phosphatidylinositol 3,4,5-trisphosphate (PIP3) (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...