Robo4 (BC071193) Mouse Tagged ORF Clone

CAT#: MR211107

  • TrueORF®

Robo4 (Myc-DDK-tagged) - Mouse roundabout homolog 4 (Drosophila) (cDNA clone MGC:78016 IMAGE:5060473)

ORF Plasmid: DDK tGFP



  "BC071193" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1200012D01Rik; AI593217
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211107 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCTGGAGGAACGGGCCTCCTGGGGACGGAGTGGCCTCTGCCTCTGCTGCTGCTTTTCATCATGG
TCCTCCAGGAGGACTTCCAGATCCAACCTCGGGACACAGTGGCCGTGGTGGGAGAGAGCTTGGTTCTTGA
GTGTGGTCCTCCCTGGGGCTACCCAAAACCCTCGGTCTCATGGTGGAAAGACGGGAAACCCCTGGTCCTC
CAGCCAGGGAGGCGCACAGTATCTGGGGATTCCCTGATGGTGTCAAGAGCAGAGAAGAATGACTCGGGGA
CCTATATGTGTATGGCCACCAACAATGCTGGGCAACGGGAGAGCCGAGCAGCCAGGGTGTCTATCCAGGA
ATCCCAGGACCACAAGGAACATCTAGAGCTTCTGGCTGTTCGCATTCAGCTGGAAAATGTGACCCTGCTA
AACCCCGAACCTGTAAAAGGTCCCAAGCCTGGGCCATCCGTGTGGCTCAGCTGGAAGGTGAGCGGCCCTG
CTGCACCTGCTGAGTCATACACAGCTCTGTTCAGGACTCAGAGGTCCCCCAGGGACCAAGGATCTCCATG
GACAGAGGTGCTGCTGCGTGGCTTGCAGAGTGCAAAGCTTGGGGGTCTCCACTGGGGCCAAGACTATGAA
TTCAAAGTGAGACCGTCCTCCGGCCGGGCTCGAGGCCCTGACAGCAATGTGTTGCTCCTGAGGCTGCCTG
AACAGGTGCCCAGTGCCCCACCTCAAGGAGTGACCTTAAGATCTGGCAACGGTAGTGTCTTTGTGAGTTG
GGCTCCACCACCTGCTGAAAGCCATAATGGTGTCATCCGTGGTTACCAGGTCTGGAGCCTGGGCAATGCC
TCATTGCCTGCTGCCAACTGGACCGTAGTGGGTGAACAGACCCAGCTGGAGATCGCCACACGACTGCCAG
GCTCCTATTGTGTGCAAGTGGCTGCAGTCACTGGAGCTGGTGCTGGAGAACTCAGTACCCCTGTCTGCCT
CCTTTTAGAGCAGGCCATGGAGCAATCAGCACGAGACCCCAGGAAACATGTTCCCTGGACCCTGGAACAG
CTGAGGGCCACCTTGAGACGACCAGAAGTCATTGCCAGTAGTGCTGTCCTACTCTGGTTGCTGCTACTAG
GCATTACTGTGTGTATCTACAGACGACGCAAAGCTGGGGTGCACCTGGGCCCAGGTCTGTACAGATACAC
CAGCGAGGACGCCATTCTAAAACACAGGATGGACCACAGTGACTCCCCATGGCTGGCAGACACCTGGCGT
TCCACCTCTGGCTCTCGAGACCTGAGCAGCAGCAGCAGCCTTAGTAGTCGGCTGGGATTGGACCCTCGGG
ACCCACTAGAGGGCAGGCGCTCCTTGATCTCCTGGGACCCTCGGAGCCCCGGTGTACCCCTGCTTCCAGA
CACGAGCACGTTTTACGGCTCCCTCATTGCAGAGCAGCCTTCCAGCCCTCCAGTCCGGCCAAGCCCCAAG
ACACCAGCTGCTAGGCGCTTTCCATCCAAGTTGGCTGGAACCTCCAGCCCCTGGGCTAGCTCAGATAGTC
TCTGCAGCCGCAGGGGACTCTGTTCCCCACGCATGTCTCTGACCCCTACAGAGGCTTGGAAGGCCAAAAA
GAAGCAGGAATTGCACCAAGCTAACAGCTCCCCACTGCTCCGGGGCAGCCACCCCATGGAAATCTGGGCC
TGGGAGTTGGGAAGCAGAGCCTCCAAGAACCTTTCTCAAAGCCCAGGTCCAAACTCTGGTTCCCCAGGAG
AAGCGCCCCGAGCCGTGGTATCCTGGCGTGCTGTGGGACCACAACTTCACCGCAACTCCAGTGAGCTGGC
ATCTCGTCCACTCCCTCCAACACCCCTTTCTCTTCGTGGAGCTTCCAGTCATGACCCACAGAGCCAGTGT
GTGGAGAAGCTCCAAGCTCCCTCCTCTGACCCACTGCCAGCAGCCCCTCTCTCCGTCCTCAACTCTTCCA
GACCTTCCAGCCCCCAGGCCTCTTTCCTCTCCTGTCCTAGCCCATCCTCCAGCAACCTGTCCAGCTCCTC
GCTGTCATCCTTAGAGGAGGAGGAGGATCAGGACAGCGTGCTCACCCCCGAGGAGGTAGCCCTGTGTCTG
GAGCTCAGTGATGGGGAGGAGACACCCACGAACAGTGTATCTCCTATGCCAAGAGCTCCTTCCCCGCCAA
CAACCTATGGCTATATCAGCATACCAACCTGCTCAGGACTGGCAGACATGGGCAGAGCTGGCGGGGGCGT
GGGGTCTGAGGTTGGGAACTTACTGTATCCACCTCGGCCCTGCCCCACCCCTACACCCAGCGAGGGCTCC
CTGGCCAATGGTTGGGGCTCAGCTTCTGAGGACAATGTCCCCAGCGCCAGGGCCAGCCTGGTTAGCTCTT
CTGATGGCTCCTTCCTCGCTGATACTCACTTTGCTCGTGCCCTGGCAGTGGCTGTGGATAGCTTTGGCCT
CAGTCTGGATCCCAGGGAAGCTGACTGTGTCTTCACTGATGCCTCATCACCTCCCTCCCCTCGGGGTGAT
CTCTCCCTGACCCGAAGCTTCTCTCTGCCTTTGTGGGAGTGGAGGCCAGACTGGTTGGAAGATGCTGAGA
TCAGCCACACCCAGAGGCTGGGGAGGGGGCTGCCTCCCTGGCCTCCTGATTCTAGGGCCTCTTCCCAGCG
AAGTTGGCTAACTGGTGCTGTGCCCAAGGCTGGTGATTCCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211107 protein sequence
Red=Cloning site Green=Tags(s)

MGSGGTGLLGTEWPLPLLLLFIMVLQEDFQIQPRDTVAVVGESLVLECGPPWGYPKPSVSWWKDGKPLVL
QPGRRTVSGDSLMVSRAEKNDSGTYMCMATNNAGQRESRAARVSIQESQDHKEHLELLAVRIQLENVTLL
NPEPVKGPKPGPSVWLSWKVSGPAAPAESYTALFRTQRSPRDQGSPWTEVLLRGLQSAKLGGLHWGQDYE
FKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQGVTLRSGNGSVFVSWAPPPAESHNGVIRGYQVWSLGNA
SLPAANWTVVGEQTQLEIATRLPGSYCVQVAAVTGAGAGELSTPVCLLLEQAMEQSARDPRKHVPWTLEQ
LRATLRRPEVIASSAVLLWLLLLGITVCIYRRRKAGVHLGPGLYRYTSEDAILKHRMDHSDSPWLADTWR
STSGSRDLSSSSSLSSRLGLDPRDPLEGRRSLISWDPRSPGVPLLPDTSTFYGSLIAEQPSSPPVRPSPK
TPAARRFPSKLAGTSSPWASSDSLCSRRGLCSPRMSLTPTEAWKAKKKQELHQANSSPLLRGSHPMEIWA
WELGSRASKNLSQSPGPNSGSPGEAPRAVVSWRAVGPQLHRNSSELASRPLPPTPLSLRGASSHDPQSQC
VEKLQAPSSDPLPAAPLSVLNSSRPSSPQASFLSCPSPSSSNLSSSSLSSLEEEEDQDSVLTPEEVALCL
ELSDGEETPTNSVSPMPRAPSPPTTYGYISIPTCSGLADMGRAGGGVGSEVGNLLYPPRPCPTPTPSEGS
LANGWGSASEDNVPSARASLVSSSDGSFLADTHFARALAVAVDSFGLSLDPREADCVFTDASSPPSPRGD
LSLTRSFSLPLWEWRPDWLEDAEISHTQRLGRGLPPWPPDSRASSQRSWLTGAVPKAGDSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC071193
ORF Size 2703 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC071193, AAH71193
RefSeq Size 3419 bp
RefSeq ORF 2705 bp
Locus ID 74144
MW 96.7 kDa
Gene Summary Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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