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Nfatc4 (NM_023699) Mouse Tagged ORF Clone
CAT＃: MR211104
- TrueORF®
Nfatc4 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1
"NM_023699" in other vectors (3)
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CNY 6,600.00
CNY 7,890.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3110041H08Rik; AW107667; AW546455; Nfat3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211104 representing NM_023699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGCAAGCTGCGAGGATGAGGAGCTGGAATTTAAGCTGGTGTTCGGAGAGGAAAAGGAGCCCC CCCCGCTGGGCCCTGGGGGTCCTGGGGAAGAGCTGGACTCAGAGGATACCCCTCCATGCTGTCGTTTGGC CCTGGGGGAACCCCTTCCTTATGGTGCTGCCCCTATTGGGATTCCCCGACCCCCACCCCCTCGGCCAGGG ATGCACTCACCACCGCCCCGTCCCGCCCCTTCACCTGGCACCTGGGAGAGCCAGCCAGCTAGGTCGGTGA GGCTGGGGGGCCCAGGAGGTAATGCAGGGGGTGCTGGGGGTGGTCGTGTTCTTGAGTGTCCCAGCATCCG GATTACCTCCATCTCTCCCACGCCTGACCCGCCGACCTCGCTAGAGGACACATCTGAAACTTGGGGGGAC GGCTCTCCTAGAGATTACCCTCCACCAGAAGGCTTTGGGGGCTATCGAGAGGCTGGAGGTCAGGGCGGAG GCGCTTTCTTCAGCCCGAGCCCTGGCAGCAGCAGCCTGTCTTCGTGGAGCTTCTTCTCGGACGCCTCCGA CGAGGCAGCCCTGTATGCAGCGTGCGACGAGGTGGAGTCTGAACTTAATGAAGCAGCCTCCCGTTTTGGC TTGAGCTCTCCGCTGCCCTCGCCCCGGGCTTCTCCTAGGCCATGGACCCCGGAGGATCCCTGGAGCCTAT ACGGTCCAAGCTCGGGAGGCCGAGCGCCAGAGGATAGCTGGCTACTCCTCAGCGCTCCTGGGCCCGTCCC AGCCTCCCCTCGGCCTGCTTCTCCCTGCGGCAAGCGGCGCTATTCCAGTTCCGGAACCCCATCTTCGGCC TCCCCAGCTTTGTCCCGCCGAGGCAGCCTTGGGGAAGAAGGTCCAGAGCCACCTCCACCACCCCCGTTGC CTCTGGTCCGGGACCCTAGTTCCCCTGGCCCTTTTGACTATGTGGGTGCTCCACCAACCGAGAGCATCCC TCAGAAAACTCGGAGGACTTCTAGCGAGCAAGCAGTGGCCCTGCCTCGGTCTGAAGAGCCTCCCTCGTGC AATGGGAAGCTACCCTCCGGTACAGAGGACTCTGTGGCTGCTCCGGGGGCTCTGCGGAAAGAGGTGGCCG GCATGGATTACCTAGCAGTGCCTTCTCCCCTTGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCCAT CTTCAGGACCTCTGCCCTACCTCCCCTGGACTGGCCTTTACCCAGCCAGTATGAGCAGCTGGAGCTGAGG ATCGAGGTACAGCCTAGAGCACACCACCGAGCTCACTATGAGACTGAGGGCAGCCGGGGGGCTGTCAAGG CTGCTCCTGGAGGCCACCCTGTGGTCAAGCTCCTAGGCTACAGTGAGAAGCCATTGACTCTGCAGATGTT CATTGGCACTGCAGATGAGAGGAGCCTGCGGCCCCATGCCTTCTACCAGGTGCACCGGATTACTGGCAAG ATGGTGGCTACAGCCAGCTATGAAGCTGTAGTCAGTGGTACCAAAGTGTTGGAGATGACCTTACTCCCAG AGAACAACATGGCTGCCAACATTGACTGTGCTGGAATCCTGAAGCTTCGAAATTCAGACATTGAGCTACG GAAGGGTGAGACAGACATCGGGCGCAAAAACACGCGTGTGCGGCTGGTGTTCCGTGTGCACGTGCCTCAG GGCGGCGGGAAGGTCGTGTCTGTGCAGGCAGCATCAGTGCCCATCGAGTGCTCCCAGCGCTCGGCCCAGG AGCTGCCCCAGGTGGAGACCTACAGCCCCAGTGCCTGCTCTGTGAGAGGAGGGGAGGAGCTAGTGTTGAC TGGTTCCAACTTCCTGCCAGACTCTAAAGTGGTGTTCATTGAGAGGGGTCCTGATGGAAAACTGCAGTGG GAGGAAGAGGCAGCGGTGAACCGACTGCAGAGCAGTGAGGTGACATTGACTCTCACCATCCCCGAGTACA GCAACAAGCGGGTGTCCCGACCAGTCCAGGTCTACTTTTACGTCTCCAATGGACGGAGGAAGCGCAGTCC TACCCAAAGTTTCAAGTTCCTACCTGTGGTCTTCAAGGAGGAGCCCCTACCAGACTCATCTCTCCGGGGC TTCCCTTCCACATCGGGTCCCCCCTTTGGCCCTGACGTGGACTTCTCACCGCCCAGGCCCCCTTACCCCT CCTATCCCCATGAAGACCCTGCTTATGAGACTCCTTATCTGTCAGAAGGCTTTGGTTATAGCACACCCGC TCTGTACCCCCAGACGGGGCCCCCACCGTCCTATAGATCTGGCCTGCGGATGTTCCCTGAGACTGGGGGT ACCACAGGTTGTGCCCGCCTACCTTCAGTCTCTTTCCTTCCGCGCCCCTTTCCTGGTGATCAATATGGAG GACAGGGCTCCTCTTTTGCCCTGGGGCTTCCATTCTCCCCTCCAGCCCCCTTTAGGCCGCCACTGCCTTC CTCCCCACCACTCGAAGACCCCTTCCATCCCCAGAGTGCCATCCACCCCTTACCTCCTGAGGGCTACAAT GAGGTGGGGCCAGGCTATACCCCTGGGGAGGGGGCTTCGGAGCAGGAGAAAGCCAGGGGTGGCTACAGCA GCGGCTTCAGAGACAGTGTACCTATCCAGGGTATCACCCTGGAGGAAGTGAGTGAGATCATTGGCCGAGA CCTGAGTGGCTTCCCTGCACGTCCTGGAGAAGAGCCTCCTGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211104 representing NM_023699
Red=Cloning site Green=Tags(s) MGAASCEDEELEFKLVFGEEKEPPPLGPGGPGEELDSEDTPPCCRLALGEPLPYGAAPIGIPRPPPPRPG MHSPPPRPAPSPGTWESQPARSVRLGGPGGNAGGAGGGRVLECPSIRITSISPTPDPPTSLEDTSETWGD GSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFG LSSPLPSPRASPRPWTPEDPWSLYGPSSGGRAPEDSWLLLSAPGPVPASPRPASPCGKRRYSSSGTPSSA SPALSRRGSLGEEGPEPPPPPPLPLVRDPSSPGPFDYVGAPPTESIPQKTRRTSSEQAVALPRSEEPPSC NGKLPSGTEDSVAAPGALRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELR IEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERSLRPHAFYQVHRITGK MVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQ GGGKVVSVQAASVPIECSQRSAQELPQVETYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQW EEEAAVNRLQSSEVTLTLTIPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFKFLPVVFKEEPLPDSSLRG FPSTSGPPFGPDVDFSPPRPPYPSYPHEDPAYETPYLSEGFGYSTPALYPQTGPPPSYRSGLRMFPETGG TTGCARLPSVSFLPRPFPGDQYGGQGSSFALGLPFSPPAPFRPPLPSSPPLEDPFHPQSAIHPLPPEGYN EVGPGYTPGEGASEQEKARGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPARPGEEPPA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023699 |
| ORF Size | 2703 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023699.2 |
| RefSeq Size | 3284 bp |
| RefSeq ORF | 2706 bp |
| Locus ID | 73181 |
| UniProt ID | Q8K120 |
| MW | 95.8 kDa |
| Gene Summary | Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed:17198697). Along with NFATC3, involved in embryonic heart development (PubMed:12750314, PubMed:17198697). Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (PubMed:12750314). Transactivates many genes involved in heart physiology. Along with GATA4, binds to and activates NPPB/BNP promoter (PubMed:9568714). Activates NPPA/ANP/ANF and MYH7/beta-MHC transcription (By similarity). Binds to and transactivates AGTR2 gene promoter (PubMed:17198697). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (PubMed:22586092). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during a developmental critical period when auditory neurons depend on afferent input for survival (PubMed:18354019). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP). Plays a role in adipocyte differentiation. May be involved in myoblast differentiation into myotubes (By similarity). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription. Binds to PPARG gene promoter and regulates its activity (By similarity). Binds to PPARG and REG3G gene promoters (PubMed:17198697).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ppt1-deficiency dysregulates lysosomal Ca ++ homeostasis contributing to pathogenesis in a mouse model of CLN1 disease.
,null,
Journal of inherited metabolic disease
,PubMed ID 35150145
[Nfatc4]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204560 | Nfatc4 (untagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1, (10ug) |
CNY 6,608.00 |
|
| MR211104L3 | Lenti ORF clone of Nfatc4 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1 |
CNY 9,790.00 |
|
| MR211104L4 | Lenti ORF clone of Nfatc4 (mGFP-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1 |
CNY 9,790.00 |
