Zfp451 (BC062154) Mouse Tagged ORF Clone

CAT#: MR211090

  • TrueORF®

Zfp451 (Myc-DDK-tagged) - Mouse zinc finger protein 451 (cDNA clone MGC:70050 IMAGE:6331597)

ORF Plasmid: DDK tGFP



  "BC062154" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,930.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; Kiaa0576-hp; mKIAA1702; Znf451
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211090 representing BC062154
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAGGTCTGAGAAGCCAATTTTGTGTCCTATAATGCACTGTAACAAGGAATTTGACAATGGGCACC
TTCTGTTGGGACATTTGAAAAGGTTTGATCACTCCCCATGTGATCCCACAATTACGTTACATGGACCTCT
GGCTAATTCGTTTGCATGTGCAGTGTGCTATGAACATTTCGTTACTCAACAGCAGTACAAGGACCATCTT
CTCTCCAGGACAGCTGCAGCCGATGGACATAGCAATAGCCTTCTTCCTCAGATTATTCAATGTTATGCTT
GTCCACAGTGCTTCCTTTTGTTTAGCACCAAGGATGAGTGTTTGAAGCATATGTCTACAAAGAATCATTT
CCATCAGAGCTTTAAACTGAGTGATAATAAAGGAACGGCCCGCCCAATATCATTTCCATCTTTTGCAAAG
AAGCGTTTGGTCTCTCTGTGCAAAGATGTTCCATTTCAGGTTAAGAGTGTGGCCTGCCACCAGACTCTGC
GTTCTCACATGGAGCTCACTGCCCATTTCAGGGTTCGTTGTCAAAATGCTGGACCTGTTGCTATAGCTGA
AAAAAGCATTACTCAGGTTGCAAAAGAATTCATAGTAAGAGGTTATTGTTCAGATTGCAACCAGGTCTTT
ATGGATGTAGCCAGCACCCAAAGTCACAAGAATTCAGGACACAAAATTACACTTGCGAACTCGGTGGAAG
AATCTGTCTTGCTTTATTGCCACATCAGTGAAGGGAGTCGGCCTCCTTGTGATTTACATTTATTTAGTCA
ACCAAAAATTTCATCACTTAAACGGATTCTGTCCGTTAAAGAGTCCAGTGCAGAGGATTGTATCGTTCCG
ACAAAGAAGGTGAACTTAGGTGTTGAAAGCCTTGGAGGTGCAACTCGTGTGCAGAGGCAGAGTCCAGCAG
TCACAGCCTGGTTTTGTGAATGCAGACGGCAGTTTCCCAGTGAAGAGGCGGTAGAAAAGCATGTTTTCTC
GGCAAACACAATGTGTTATAAGTGTGTGGTCTGTGGAAAGGTTTGTGAAGATTCGGGGGTCATGCGTTTA
CACATGAGCCGGTTTCATGGAGGGGCGCATTTAAATAACTTTCTATTTTGGTGTCGGACGTGCAAAAAGG
AGTTAGTAAAGAAAGATGCCATCATGGCACACATTACTGAGTTTCATAGTGGGCATAGATATTTTTATGA
GATGGATGAGGTAGAGGAGGAAGAGGAGGAGGCCATGCCGTCATCCTCTGTGGAGAGCCATTTGAATACT
GACAAACCTCCTTCACCCATTGCTGTTGTTGATCACTGCCCGGCCAACAGTCCTCCCAGGGGCAGGTGGC
AGTGCCGCATCTGTGAGGACATGTTTGAATCCCAGGAGTGTGTGAAGCAGCACTGCATGTCCTTGACAAG
CCACCGGTTTCACAGATACAGCTGTGCCCACTGCAGAAAGACTTTCCATAAAGTGGAAACTCTGTACCGA
CATTGCCAGGACGAGCATGACAGTGAGATCATGATGAAGTACTTCTGTGGGCTTTGTGATCTCATTTTTA
ACAAGGAAGAAGAATTTCTGAGTCACTATAAGGAACACCACAGCATAGATTATGTGTTTGTGTCAGAAAA
AACTAAAACCTCAATTAAAACTGAAGGCGATTTTAAAATAGTAGAGACCAGTAGTTTACTAAGCTGTGGC
TGTCATGAGAGTTACATGTGTAAAATCAACAGAAAGGAAGACTATGACCGATGCCTTCCAGTCCTGCTGG
AGAAAGGTAGGCTGTGGTTTCGCTGCAGCTCGTGCTCAGCCACAGCACAGAATGTGACCGACATTAACAC
TCACGTCTGCCAAGTGCACAGAAAAGAGAAAAGTGAGGAGGAGCAGCAGTATGTGATCAAGTGTGGCATC
TGCACCAAGGCATTCCAGAACACGGAGAGCGCTCAGCAGCACTTCCACAGGAAGCACGCGGCCCTCCAGA
AACCCACCGCGACCCCAGGGGGAGCCAACAGAAGCAGCACATGCCAGCTGGCTGCTAGTGCCTCACATGC
TGAGAAAAACCTGAAACAGCCTAGCTCTCAGAAACATTCAGACGTGGAAAAAGGAGCTGAGCATGATGTA
CGCTGCCAGAACATAGAGGAGGAAGTTGAGCTTCCGGATGTGGACTACCTGCGGACCATGACTCACATAG
TCTTTGTAGATTTTGATAACTGGTCCAACTTTTTTGGTCATCTACCTGGGCATCTTAATCAAGGAACGTT
TATTTGGGGCTTTCAAGGGGGAAACACCAACTGGAAGCCCCCGCTCAGCTGTAAGGTCTATAATTACTTG
AGCAGGATTGGCTGCTTCTTCCTTCATCCTCGCTGCAGTAAAAGAAAAGATGCCGCGGATTTTGCCATAT
GTATGCATGCTGGCCGTCTAGATGAGCAACTTCCCAAACAAATTCCTTTCACCATCCTCTCGGGAGACCA
AGGCTTTCTGGAGCTAGAGAATCAATTTAAGAAGACCCAGAGGCCAGCTCATATACTAAACCCTCACCAC
TTAGAGGGAGACATGATGTGTGCCTTGTTAAATAGCATATCTGATACTACCAAAGAGTGTGACAGTGACG
ATAGCTCGGGGATGAAAGGATCTCCAGCAGAGGAGCTCAGGGCCACGGAGGATGTGGAATTAGAAGAAGC
TATTAGAAGAAGTCTTGAGGAGATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211090 representing BC062154
Red=Cloning site Green=Tags(s)

MWRSEKPILCPIMHCNKEFDNGHLLLGHLKRFDHSPCDPTITLHGPLANSFACAVCYEHFVTQQQYKDHL
LSRTAAADGHSNSLLPQIIQCYACPQCFLLFSTKDECLKHMSTKNHFHQSFKLSDNKGTARPISFPSFAK
KRLVSLCKDVPFQVKSVACHQTLRSHMELTAHFRVRCQNAGPVAIAEKSITQVAKEFIVRGYCSDCNQVF
MDVASTQSHKNSGHKITLANSVEESVLLYCHISEGSRPPCDLHLFSQPKISSLKRILSVKESSAEDCIVP
TKKVNLGVESLGGATRVQRQSPAVTAWFCECRRQFPSEEAVEKHVFSANTMCYKCVVCGKVCEDSGVMRL
HMSRFHGGAHLNNFLFWCRTCKKELVKKDAIMAHITEFHSGHRYFYEMDEVEEEEEEAMPSSSVESHLNT
DKPPSPIAVVDHCPANSPPRGRWQCRICEDMFESQECVKQHCMSLTSHRFHRYSCAHCRKTFHKVETLYR
HCQDEHDSEIMMKYFCGLCDLIFNKEEEFLSHYKEHHSIDYVFVSEKTKTSIKTEGDFKIVETSSLLSCG
CHESYMCKINRKEDYDRCLPVLLEKGRLWFRCSSCSATAQNVTDINTHVCQVHRKEKSEEEQQYVIKCGI
CTKAFQNTESAQQHFHRKHAALQKPTATPGGANRSSTCQLAASASHAEKNLKQPSSQKHSDVEKGAEHDV
RCQNIEEEVELPDVDYLRTMTHIVFVDFDNWSNFFGHLPGHLNQGTFIWGFQGGNTNWKPPLSCKVYNYL
SRIGCFFLHPRCSKRKDAADFAICMHAGRLDEQLPKQIPFTILSGDQGFLELENQFKKTQRPAHILNPHH
LEGDMMCALLNSISDTTKECDSDDSSGMKGSPAEELRATEDVELEEAIRRSLEEM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062154
ORF Size 2685 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC062154.1
RefSeq Size 3597 bp
RefSeq ORF 2687 bp
Locus ID 98403
MW 131.9 kDa
Gene Summary E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins. Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11'. Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9'. Plays a role in regulating the transcription of AR targets (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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