G430002G23Rik (BC004660) Mouse Tagged ORF Clone

CAT#: MR211030

  • TrueORF®

G430002G23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA G430002G23 gene (cDNA clone MGC:7714 IMAGE:3497913)

ORF Plasmid: DDK tGFP



  "BC004660" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,740.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms G430002G23Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211030 representing BC004660
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCAGTGTGGAAGCGCCTGCAGCGGGTTGGCAAGCGGGCCGCCAAGTTCCAGTTTGTGGCGTGTT
ACCATGAACTGGTGTTGGAGTGCACCAAGAAGTGGCAGCCTGACAAGCTGGTGGTGGTATGGACTCGGCG
GAACCGAAGGATCTGCTCCAAGGCCCACAGCTGGCAGCCTGGCATCCAGAACCCATACCGTGGCACCGTG
GTATGGATGGTTCCTGAGAACGTGGACATCTCGGTGACCTTGTACAGGGACCCTCATGTGGACCAGTATG
AAACCAAAGAGTGGACTTTTATTATTGAAAATGAGTCCAAGGGACAGCGGAAGGTACTGGCCACAGTTGA
TGTGAACCTAGCCCACCATGCAGGGCCTGTGCCTGCTCAGGTTCCACTTCGACTGCGGCTGAAGCCCAAA
TCTGTGAAGGTGGTGCATGCCGAACTGAGCCTCACCCTTTCTGGGGTGCTGCTGCGAGAAGGCCGTGCCA
CGGATGATGACATGCAGAGTCTGGCCAGCCTCATGAGTGTGAAGCCTAGTGACGTGGGAAACCTGGATGA
CTTTGCTGAGAGTGATGAGGAGGAAGCTAATGGCCCTGGGGCTCCTGAGGTCCGGACTCGAGGCCCCCAG
TCAGATCTGTCTCGAGAGCTGAAGACACTCTGTGAAGAAGAAGATGAAGGCCACATACGGCCCCAGCAGG
CAGCTGCCAGACCCTCTAGTGCTGAAGACACCAGCCCTGCCCCAGTGAGTGCCCCTGCACCCCCGGTCAG
GGCCTTCCGGGGCCAGGGGTCAGAACCAGCTGCTATAACCGGGGGCCAGGTGGGGCCTGAAACCCCAGAG
CCCCCGCCATCCCCACCAGAGACAAGGTCCACTGGGCAGCCAGGCCAGACCATGGTCCCCACCCCAGCCC
CTCGGCTCCGGAAAGGCTCTGATGCACCCTCGTCCCCAGTCCCCTGCAGTGGGGATGAGGTCCCCAATAC
CTCAGAGGATCCTCCAACAGGAATGGGCTCTTCTGGGGAGACCCAGGCTCAGATAAGCTCTCAGGAAGGG
ACAGAGGCCCATGAAGCCAGGCCAGAACCAGACATTGAGGCACCACCTACCCTGGTCAGCTCCAGCCAGT
CCCTGTTGGAGTGGTGCCAAGAGGTCACCAACGGCTACCGTGGTGTCTGCATCACCAACTTCACCACGTC
CTGGCGCAATGGCCTGGCCTTCTGTGCTATTTTACATCGATTCTACCCAGACAAGATCGATTATTTCTCC
CTTGATCCCCTCAACATCAAACAGAACAACAAGCAGGCTTTTGATGGCTTCGCTGCCCTGGGTGTGTCTC
GGCTGCTCGAGCCGGCGGACATGGTACTTCTGTCCGTACCTGACAAGCTCATCGTCATGACGTACCTGTG
CCAGATCCGTGCCTTCTGCACTGGGCAGGAGCTGCAGCTGGTGCAACTGGAGGGCGGCGGTGGCTCTGGC
ACTTATCGTGTGGGCAACGCCCAGCCGAGCCTGCCCGACTGTCTGGACGCAGGAGACCTGGCGCAGCGAT
TACGCGAGCATGGGGCTGAAGTGCCCACAGAACCTAAGGAGGCTGTGAACCGCGGGACTGGGGCAATACC
AAAGGTGGCCTCCAGGGACACGGACCTGAGCTGCTCCTCTAAGGATGGGGAGGCAGAGGTTGCCCAGGAA
GCAATCCCTCAAGAGGCGCCCACCGACGGCCCTAGAGCCAGGTCGTCCACAACCCCGGTGGTCCCTGCAG
AGGGGCTGGTGAACGGAGTGGGGGCGTCAGGTGGTGTGAGACTGAGACGGTCCTCTGTCAATGGGGAGGC
TGGGCCAGTACCTCCACCCCGAGCACATGGCTCTTTCTCCCACGTGCGGGACGCCGATTTGCTAAAGAAG
AGGCGATCGAGGTTAAGGAATAGTAACTCTTTCTCTGTGGATGACCAGGACTCTGGAGCTGCAGTTGGAG
CAGGGCCTGCAGGGCCTGGAGCTGTGGAAGGTCCAAACCCTGCCTCCAGCCCTGACGCTAACCCACTCCC
AGCCCCAGTCCCACAGCAGCCGCCCGGTGGGCCCCCTCCTACTGAGGAGTCATCACCCAGCCTGGGGGAA
GAGGCAGGCCTGCAACGGTTCCAGGACACAAGTCAGTACGTGTGCGCAGAGCTGCAAGCCCTGGAGCAGG
AACAGGGACAGATAGACGGGAGGGCCGCTGAGGTGGAGAAGCAGCTGAGGAGCCTCATGGAATCAGGTGC
CAACAGGCTGCAGGAGGAGGTGCTGATTCAGGAATGGTTCACCCTGGTCAACAAGAAGAATGCGCTCATC
CGGAGGCAGGACCAGCTGCAGCTGCTCATCGAGGAGCAAGACTTGGAGCGGAGGTTTGAACTGCTGAGCC
GAGAGTTGCGGGCCATGCTGGCCATTGAAGAGTGGCAGAAAACAGTTGCGCAGCAGCACCGCGAGCAACT
CCTGTTGGAGGAGCTGGTGTCTCTGGTGAACCAGAGGGATGAACTGGTCCGGGACCTGGACCAGAAGGAA
CGGATCGCTCTGGAGGAAGATGAGCGCCTAGAACGAGGCCTGGAGCAGAGACGTCGCAAGGTGAGCCGGC
AGCTGAGCAGGCGGGAACGCTGTACCCTGAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211030 representing BC004660
Red=Cloning site Green=Tags(s)

MTSVWKRLQRVGKRAAKFQFVACYHELVLECTKKWQPDKLVVVWTRRNRRICSKAHSWQPGIQNPYRGTV
VWMVPENVDISVTLYRDPHVDQYETKEWTFIIENESKGQRKVLATVDVNLAHHAGPVPAQVPLRLRLKPK
SVKVVHAELSLTLSGVLLREGRATDDDMQSLASLMSVKPSDVGNLDDFAESDEEEANGPGAPEVRTRGPQ
SDLSRELKTLCEEEDEGHIRPQQAAARPSSAEDTSPAPVSAPAPPVRAFRGQGSEPAAITGGQVGPETPE
PPPSPPETRSTGQPGQTMVPTPAPRLRKGSDAPSSPVPCSGDEVPNTSEDPPTGMGSSGETQAQISSQEG
TEAHEARPEPDIEAPPTLVSSSQSLLEWCQEVTNGYRGVCITNFTTSWRNGLAFCAILHRFYPDKIDYFS
LDPLNIKQNNKQAFDGFAALGVSRLLEPADMVLLSVPDKLIVMTYLCQIRAFCTGQELQLVQLEGGGGSG
TYRVGNAQPSLPDCLDAGDLAQRLREHGAEVPTEPKEAVNRGTGAIPKVASRDTDLSCSSKDGEAEVAQE
AIPQEAPTDGPRARSSTTPVVPAEGLVNGVGASGGVRLRRSSVNGEAGPVPPPRAHGSFSHVRDADLLKK
RRSRLRNSNSFSVDDQDSGAAVGAGPAGPGAVEGPNPASSPDANPLPAPVPQQPPGGPPPTEESSPSLGE
EAGLQRFQDTSQYVCAELQALEQEQGQIDGRAAEVEKQLRSLMESGANRLQEEVLIQEWFTLVNKKNALI
RRQDQLQLLIEEQDLERRFELLSRELRAMLAIEEWQKTVAQQHREQLLLEELVSLVNQRDELVRDLDQKE
RIALEEDERLERGLEQRRRKVSRQLSRRERCTLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC004660
ORF Size 2622 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC004660.1
RefSeq Size 3183 bp
RefSeq ORF 2624 bp
Locus ID 114601
MW 116.7 kDa
Gene Summary May act as Rab effector protein and play a role in vesicle trafficking (By similarity). Involved in apical-directed transport in polarized epithelial cells; the functions seems to implicate Rab8, BIN1 and possibly DNM1 (PubMed:26833786).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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