L3mbtl3 (BC082309) Mouse Tagged ORF Clone

CAT#: MR210974

  • TrueORF®

L3mbtl3 (Myc-DDK-tagged) - Mouse l(3)mbt-like 3 (Drosophila) (cDNA clone MGC:90546 IMAGE:30356672)

ORF Plasmid: DDK tGFP



  "BC082309" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,288.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI481284; MBT-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210974 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGAATCTGCATCTAGCACAAGTGGTCAAGAGTTTGATGTGTTTAGTGTTATGGACTGGAAGGATG
GCGTTGGTACCCTACCAGGGAGTGATTTAAAGTTTCGAGTAAATGAGTTTGGAGCTTTGGAAGTTATCAC
AGACGAAAGCGAGATGGAAAGTGTTAAGAAAGCTACAGCCACTACCACATGGATGGTCCCAACTGCTCAA
GACGTCTTTTCAGAGAAGACTGGGGTACCCTTCAGGCTGAAGGAGCAATCAAAAGCAGACGGGCTTCAGT
TTTGTGAGAACTGTTGTCAGTATGGCAACGGAGATGAATGTCTTTCGGGAGGAAAGTATTGCAGCCAGAA
TTGTGCTCGGCATGCCAAAGACAAAGATCAAAAGGATGAACGGGATGGAGGCGAAGACAACGATGAGGAA
GATCCTAAGTGTAGTCGAAAGAAAAAGCCAAAATTGTCTCTGAAGGCGGACTCCAAGGACGATGGAGAAG
AGAGAGATGATGAAATGGAGAACAAACAAGATGGAAGGATCCTGAGGGGTTCTCAGAGAGCCAGAAGGAA
AAGACGCGGGGATTCTGCCGTCTTAAAGCAAGGCTTGCCTCCTAAAGGCAAGAAGACCTGGTGTTGGGCT
TCCTACCTGGAGGAGGAGAAAGCAGTGGCTGTGCCTACGAAGCTGTTCAAAGAGCACCAATCCTTCCCAT
ATAACAAAAATGGATTCAAAGTGGGCATGAAGCTAGAAGGCGTGGACCCTGACCACCAGGCAATGTACTG
CGTCCTTACTGTGGCTGAGGTTTGTGGATATAGGATAAAACTTCACTTTGACGGTTATTCTGATTGCTAT
GACTTCTGGGTAAATGCAGATGCTCTGGACATTCACCCAGTAGGCTGGTGTGAGAAGACTGGCCATAAGC
TCCGCCCTCCAAAAGGGTATAAAGAAGAAGAATTTAATTGGCAGTCCTACCTTAAGACTTGCAAAGCCCA
GGCTGCTCCCAAGTCATTATTTGAAAATCAGAACATAACAGTGATCCCGTCAGGCTTTCGAGTTGGGATG
AAGCTCGAGGCTGCAGACAAAAAGAGCCCTTCTGTCATCTGTGTTGCTACGGTAACAGATATGGTGGACA
ATCGTTTCCTGGTACATTTTGACAACTGGGATGAGAGCTATGACTATTGGTGTGAATCAAACAGTCCACA
CATTCATCCAGTCGGCTGGTGTAAAGAGCACAGAAGAACCCTGATTACTCCACCAGGCTATTCTCATGTG
AAACACTTTTCCTGGGATAAATATTTAGAAGAAACCAATTCTTTACCTGCTCCTGCAAGAGCTTTCAAAG
TGAAACCTCCACATGGATTCCAGAAGAAAATGAAGCTGGAAGCTGTGGACAAAAGAAACCCGCTGTTCAT
CAGAGTAGCAACTGTAGCCGACACGGATGACCACCGGATAAAGGTTCACTTTGATGGCTGGAGCAGTTGC
TATGACTACTGGATTGATGCAGACTCTCCAGATATTCACCCCGTGGGCTGGTGTTCCAAGACCGGGCACC
CTCTTCAGGCTCCGCTAAGCCCTGCAGAATTAATGGAACCATCAGAAACCGGAGGATGTCCAACTCTAGG
CTGCAGGGGTGTTGGTCATTTTAAAAAATCGAGATACCTGGGCACTCAGAGTGGTGCCAACTGTCCCTAT
TCAGAAATCAACCTGAGTAAGGAACGCATCTTCCCAGACCGCCTAAGTGGTGACACCTCTCCACCTACTA
CTCCCTCGTTCCCAAGGAGTAAAAGGATGGACACCAGAGAAAGTTCCTCGTCTCCTGAAACCAGGGAAAA
GCATGCCAATAACTTCAAAGAAGATTCTGAAAAGAAAAAAGAAAATGAAGTAAAGACATCAGCCGAAGCC
AAAGTTGTTCGGGAAGAGCCTACCCCTAGTGTACAGCAGAGTCAGCCACCTCAGCAGGTGCAGCAAGTGC
AGCATGCTCAACCTCCTCAGCAGGCACAGAAGGCTCCGCAGGCCCAGCAGGCCCAGCAAGCCCAGCAGGC
CCAGCAGGCTCCACAGGCCCCACAGACTCCACAGCCCCAGCAGGCGCCACAGGTCCAGCAGGCCCAGCAG
GCTCCACAGGCTCAGCAGGCCCAGCAGCCCCAGCAGGCCCAGCAGCCTCAGCAGGCCCCACCCGTCCAAC
AGCCTCAGCAGGTTCAACAGGCTCAGCCCACCCAGCAGCAAGCCCAGACCCAGCAGCAGGCCCAGCGACG
CTCAGCAGTATTTCTCTCCTTTAAGCCTCCCATTCCGTGTCTGCCCTTGCGCTGGGAGCAGCAGAGCAAG
CTTCTTCCGACTGTCGCTGGTATCCCAGCTAGTAGAGTTTCCAAATGGAGCACAGACGAGGTGTCAGAAT
TCATTCAGAGCTTGCCTGGGTGTGAAGAACATGGAAAGGTGTTTAAGGATGAACAAATTGATGGAGAAGC
ATTTTTACTCATGACCCAAACAGACATTGTTAAAATCATGAGCATTAAGCTGGGCCCAGCTCTCAAAATT
TTCAACTCTATTCTGATGTTCAAAGCTGCGGAGAAGAATTCCCACAATGAACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210974 protein sequence
Red=Cloning site Green=Tags(s)

MTESASSTSGQEFDVFSVMDWKDGVGTLPGSDLKFRVNEFGALEVITDESEMESVKKATATTTWMVPTAQ
DVFSEKTGVPFRLKEQSKADGLQFCENCCQYGNGDECLSGGKYCSQNCARHAKDKDQKDERDGGEDNDEE
DPKCSRKKKPKLSLKADSKDDGEERDDEMENKQDGRILRGSQRARRKRRGDSAVLKQGLPPKGKKTWCWA
SYLEEEKAVAVPTKLFKEHQSFPYNKNGFKVGMKLEGVDPDHQAMYCVLTVAEVCGYRIKLHFDGYSDCY
DFWVNADALDIHPVGWCEKTGHKLRPPKGYKEEEFNWQSYLKTCKAQAAPKSLFENQNITVIPSGFRVGM
KLEAADKKSPSVICVATVTDMVDNRFLVHFDNWDESYDYWCESNSPHIHPVGWCKEHRRTLITPPGYSHV
KHFSWDKYLEETNSLPAPARAFKVKPPHGFQKKMKLEAVDKRNPLFIRVATVADTDDHRIKVHFDGWSSC
YDYWIDADSPDIHPVGWCSKTGHPLQAPLSPAELMEPSETGGCPTLGCRGVGHFKKSRYLGTQSGANCPY
SEINLSKERIFPDRLSGDTSPPTTPSFPRSKRMDTRESSSSPETREKHANNFKEDSEKKKENEVKTSAEA
KVVREEPTPSVQQSQPPQQVQQVQHAQPPQQAQKAPQAQQAQQAQQAQQAPQAPQTPQPQQAPQVQQAQQ
APQAQQAQQPQQAQQPQQAPPVQQPQQVQQAQPTQQQAQTQQQAQRRSAVFLSFKPPIPCLPLRWEQQSK
LLPTVAGIPASRVSKWSTDEVSEFIQSLPGCEEHGKVFKDEQIDGEAFLLMTQTDIVKIMSIKLGPALKI
FNSILMFKAAEKNSHNEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC082309
ORF Size 2574 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC082309, AAH82309
RefSeq Size 4280 bp
RefSeq ORF 2576 bp
Locus ID 237339
MW 96.6 kDa
Gene Summary Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility (By similarity). Required for normal maturation of myeloid progenitor cells.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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