United States
Germany
Japan
United Kingdom
China
Gabbr1 (BC056990) Mouse Tagged ORF Clone
CAT＃: MR210927
- TrueORF®
Gabbr1 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442)
ORF Plasmid: tGFP
"BC056990" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,184.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GABAbR1, bM573K1.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210927 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGGGGACCCTGTACCCCGGTGGGGTGGCCGCTGCCTCTTCTGCTGGTGATGGCGGCTGGGG TGGCTCCGGTGTGGGCCTCTCACTCCCCTCATCTCCCGCGGCCTCACCCGAGGGTCCCCCCGCACCCCTC CTCAGAACGGCGTGCAGTATACATCGGGGCGCTGTTTCCCATGAGCGGGGGCTGGCCGGGGGGCCAGGCC TGCCAGCCTGCGGTGGAGATGGCGCTGGAGGACGTTAACAGCCGCAGAGACATCCTGCCGGACTACGAGC TCAAGCTTATCCACCACGACAGCAAGTGCGACCCAGGGCAAGCCACCAAGTACTTGTATGAACTACTCTA CAACGACCCCATCAAGATCATCCTCATGCCCGGCTGCAGCTCTGTGTCCACACTGGTAGCCGAGGCTGCC CGGATGTGGAACCTTATTGTGCTCTCATATGGCTCCAGCTCACCAGCCTTGTCAAACCGACAGCGGTTTC CAACGTTCTTTCGGACACATCCATCCGCCACACTCCACAATCCCACCCGGGTGAAACTCTTCGAAAAGTG GGGCTGGAAGAAGATTGCCACCATCCAGCAGACTACCGAGGTCTTCACCTCAACACTGGATGACCTGGAG GAGCGAGTGAAAGAGGCTGGGATTGAGATCACTTTTCGACAGAGTTTCTTCTCAGATCCAGCTGTGCCTG TTAAAAACCTGAAGCGTCAAGATGCTCGAATCATCGTGGGACTTTTCTATGAGACCGAAGCCCGGAAAGT TTTTTGTGAGGTCTATAAGGAACGGCTCTTTGGGAAGAAGTATGTCTGGTTTCTCATCGGGTGGTATGCT GACAACTGGTTCAAAACCTATGACCCGTCAATCAATTGTACAGTAGAAGAGATGACTGAGGCGGTGGAGG GCCATATCACCACGGAGATTGTCATGCTGAACCCTGCCAACACCCGAAGCATTTCCAACATGACATCACA GGAATTTGTGGAGAAACTAACCAAGCGGCTGAAAAGACACCCTGAGGAGACTGGAGGCTTCCAGGAGGCA CCACTGGCCTATGATGCTATCTGGGCCTTGGCTTTGGCCTTGAACAAGACCTCTGGAGGAGGTGGCCGTT CAGGAGTGCGCCTGGAGGACTTTAACTACAACAACCAGACCATTACAGACCAAATCTACCGGGCCATGAA CTCCTCCTCCTTTGAGGGTGTTTCTGGCCACGTGGTCTTTGATGCCAGCGGCTCCCGGATGGCATGGACG CTTATCGAGCAGCTACAGGGCGGCAGCTACAAGAAGATCGGCTACTACGACAGCACCAAGGATGATCTTT CCTGGTCCAAAACAGACAAGTGGATCGGAGGGTCTCCCCCAGCCGACCAGACCTTGGTCATCAAGACATT CCGTTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTCCAGCCTGGGCATTGTTCTTGCTGTT GTCTGTCTGTCCTTTAACATCTACAACTCCCACGTTCGTTATATCCAGAACTCCCAGCCCAACCTGAACA ATCTGACTGCTGTGGGCTGCTCACTGGCACTAGCTGCTGTCTTCCCCCTTGGGCTGGATGGTTACCACAT AGGGAGAAGCCAGTTCCCATTTGTCTGCCAGGCCCGCCTTTGGCTCTTGGGCTTAGGCTTTAGTCTGGGC TATGGCTCTATGTTCACCAAGATCTGGTGGGTCCACACAGTCTTCACAAAGAAGGAGGAGAAGAAGGAAC GGAGGAAGACCCTAGAGCCTTGGAAACTGTACGCCACTGTAGGCCTGCTGGTGGGCATGGATATCCTGAC TCTTGCCATCTGGCAGATTGTGGACCCCTTGCACCGAACCATTGAGACTTTTGCCAAGGAGGAACCAAAG GAAGACATTGATGTCTCCATTCTGCCCCAGCTGGAGCACTGCAGCTCCAAGAAGATGAATACATGGCTCG GCATTTTCTATGGTTACAAAGGGCTGCTGCTGCTGCTGGGAATCTTTCTTGCTTATGAGACCAAAAGTGT GTCCACTGAAAAGATCAATGACCACAGGGCCGTGGGCATGGCTATCTACAATGTTGCGGTCCTGTGTCTC ATTACCGCTCCTGTCACCATGATCCTTTCCAGCCAGCAGGACGCAGCCTTCGCCTTTGCCTCTCTGGCCA TTGTGTTCTCTTCCTACATTACTCTGGTTGTGCTCTTTGTGCCTAAGATGCGCAGGTTGATCACTCGAGG TGAATGGCAGTCTGAAGCACAGGACACAATGAAAACAGGATCATCCACCAACAACAATGAGGAAGAGAAG TCCCGACTGTTGGAGAAGGAAAACCGTGAATTGGAAAAGATCATCGCCGAGAAAGAGGAGCGTGTCTCTG AACTGCGCCATCAGCTCCAGTCTCGGCAGCAGATCCGCTCCCGGCGCCACCCCCCAACACCCCCAGACCC CTCTGGGGGCCTTCCCAGGGGACCCTCAGAGCCCCCTGACCGGCTTAGCTGTGATGGGAGTCGAGTACAT TTGCTTTACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210927 protein sequence
Red=Cloning site Green=Tags(s) MGPGGPCTPVGWPLPLLLVMAAGVAPVWASHSPHLPRPHPRVPPHPSSERRAVYIGALFPMSGGWPGGQA CQPAVEMALEDVNSRRDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAA RMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLE ERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYA DNWFKTYDPSINCTVEEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEA PLAYDAIWALALALNKTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWT LIEQLQGGSYKKIGYYDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAV VCLSFNIYNSHVRYIQNSQPNLNNLTAVGCSLALAAVFPLGLDGYHIGRSQFPFVCQARLWLLGLGFSLG YGSMFTKIWWVHTVFTKKEEKKERRKTLEPWKLYATVGLLVGMDILTLAIWQIVDPLHRTIETFAKEEPK EDIDVSILPQLEHCSSKKMNTWLGIFYGYKGLLLLLGIFLAYETKSVSTEKINDHRAVGMAIYNVAVLCL ITAPVTMILSSQQDAAFAFASLAIVFSSYITLVVLFVPKMRRLITRGEWQSEAQDTMKTGSSTNNNEEEK SRLLEKENRELEKIIAEKEERVSELRHQLQSRQQIRSRRHPPTPPDPSGGLPRGPSEPPDRLSCDGSRVH LLYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC056990 |
| ORF Size | 2532 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC056990, AAH56990 |
| RefSeq Size | 3883 bp |
| RefSeq ORF | 2534 bp |
| Locus ID | 54393 |
| MW | 95 kDa |
| Gene Summary | Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:10773016, PubMed:10075644). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10773016, PubMed:10075644). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (By similarity). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220651 | Gabbr1 (untagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442), (10ug) |
CNY 9,120.00 |
|
| MG210927 | Gabbr1 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 5,320.00 |
|
| MR210927L3 | Lenti ORF clone of Gabbr1 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 6,750.00 |
|
| MR210927L4 | Lenti ORF clone of Gabbr1 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (cDNA clone MGC:66925 IMAGE:6821442) |
CNY 6,750.00 |
