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Ftsj3 (BC012281) Mouse Tagged ORF Clone
CAT＃: MR210907
- TrueORF®
Ftsj3 (Myc-DDK-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961)
ORF Plasmid: tGFP
"BC012281" in other vectors (4)
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CNY 8,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA537063; AU045295; C79843; D11Ertd400e; Epcs3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210907 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGAAAGGCAAAGTAGGCAAGAGCCGGCGGGACAAGTTCTATCACTTGGCCAAGGAGACTGGTT ATCGTTCGCGCTCTGCCTTCAAACTGATCCAGCTCAATCGCCGCTTTCAGTTCCTGCAGAAAGCCCGGGC CTTGTTGGACCTGTGTGCTGCGCCTGGCGGATGGCTGCAGGTGGCTGCCAAGTTTATGCCTGTATCCAGT CTTATTGTAGGAGTGGACCTCGTTCCAATCAAGCCTCTCCCCAATGTGGTGACTCTCCAGGAGGATATCA CAACAGAACGCTGTAGGCAGGCCCTGAGGAAGGAGCTGAAGACCTGGAAAGTTGATGTTGTGCTTAATGA TGGGGCTCCCAATGTGGGGGCTAGTTGGGTCCATGACGCTTACTCCCAAGCTCACCTAACTCTGATGGCT CTGCGTTTGGCTTGTGATTTTTTGGCCCGTGGTGGTTGTTTCATCACAAAAGTCTTTCGTTCCCGAGACT ATCAGCCCTTGCTGTGGATCTTCCAGCAGCTGTTCCACCGTGTCCAGGCCACGAAGCCCCAAGCCTCTCG CCATGAATCTGCAGAAATCTTTGTAGTCTGTCAAGGATTCCTGGCTCCGGACAAGGTTGATGCTAAATTC TTTGATCCCAAGTTTGCCTTTAAGGAGGTTGAAGTTCAGGCTAAAACTGTTACTGAGTTGGTGACAAGGA AGAAGCCAAAGGCTGAAGGCTATGCCGAAGGTGACCTTACTCTTTATCACCGAACCTCAGTTACGGACTT CTTGCGAGCTGCCAATCCTGTTGACTTCCTCTCCAAGGCCAGCGAAATCTCAATAGATGATGAAGAGTTG GCACAGCACCCAGCTACAACTGAAGACATTAGAGTATGCTGTCAGGACATCAAAGTGCTGGGGCGAAAGG AACTTAGGTCTCTTCTGAATTGGAGAACAAAGCTTCGGCGATATGTGGCTAAGAAGCTGAAAGAGCAAGC AAAGGCATTGGACATCAGCCTCAGCTCAGAAGAAGAAGAAGAAGGTGATGAAGAAGAGGCAGTAGCTGAG ACAAAGCAGGCGCCAGAGGAAGAAGAAGAGAGAGAGGAAGAACAGCTTAATCGGACTCTGGCAGAGATGA AAGCCCAAGAAGTGGCAGAACTCAAGAGGAAGAAGAAGAAGTTGCTTCGTGAGCAGAGGAAGCAGCGGGA GCGTGTCGAGCTGAAGATGGATTTGCCTGGGGTTTCTATTGCAGATGAGGGGGAGACAGGCATGTTCTCC CTGCGAACCATCCGTGGCCAGCAGCTATTAGAGGAAGTAACACAAGGAGACATGAACGCTGCAGACACCT TTCTGTCTGATCTGCCAAGGGATGACATCTATGTGTCAGATGCTGAGGACGATGATGATACTTCTCTGGA AAGTGACCTGGATCCAGAAGAGCTGGCAGGAGTCAGGACACACTCGGATCTGAAGGAGCAGAAGTACTTG CGGTTTACTAAAGTAGATGACAATAAAGAGGAAGGAGGAGAAAATCCATTGCTGGTACCACTGGAAGAAA AGGCAGTACTGCAGGAAGAGCAAGCTAGCCTGTGGTTCTCCAAGGACGGCTTCAGTGGGATAGAGGATGA TGCTGACGAAGCCCTGGAGATCAGTCAGGCACAGCTGCTGTACAAGAGCCGTCGGAAGGAGCAGCAGCCA ACAGATCCACCTCCACCACCTACTAACTTGAAGACTGAGAAGAAATCTCCCCAGGGCCAGAATGAGGTCC CTAAGGAGACAGAAGCCATCTTGGGGACAGAGGCTGTCACTGACCCTGGAGGGGAGGAAAGAGGCAACAG CTCAGATAGTGACAGCAGCAGTAGTGAGGATGAAGATAGCTGGAAAGTGTCCCGTGGTGTGAAGCGAGGC CGTGGCTCTAAGGCAGATGAGGATGGGTTTGAGGTGGTGCCTATTCAGGACCCAGTGAAATACCGAATTC TGGACCCTGAAGGCCTTGCTCTAGGTGCTGTTATTGCCTCTTCAAAAAAGGCAAAGAGAGACCTCATAGA TAACTCGTTCAACCGGTATGCTTTTAATGAAGAGGAGGGAGAACTTCCTGAGTGGTTTGCACAGGAAGAA AAGCAGCATCGGATACGCCAGCTGCCTGTTGATAAGAAGGAGGTGGAACATTACCGAAAACGCTGGCGGG AAATCAATGCACGGCCCATCAAGAAAGTAGCTGAGGCCAAGGCCAGAAAGAAACGGAGGATGCTGAAGAA GCTGGAACAGACCAAAAAGAAGGCAGAAGCTGTGGTGAATACAGTGGACATCTCAGAACGGGAAAAGGTG GCACAGCTGGCCAGTCCAGTACATACTGCTGGACTTGGCATAGCAGTCATTGTTAGAGTTTTTCATCTCG CCTTTAAGGGTGTGAACCACAATAATCTCCCAGGATGCAAGCCAAAAGGTCACGCAAAGATAGTTGTTGC TCGAATGAAGAAGGATCAGAGAGCACAACAACGGAAGGAACAGAAGAAAAAACACAAGCGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210907 protein sequence
Red=Cloning site Green=Tags(s) MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSS LIVGVDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMA LRLACDFLARGGCFITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGFLAPDKVDAKF FDPKFAFKEVEVQAKTVTELVTRKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEISIDDEEL AQHPATTEDIRVCCQDIKVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSEEEEEGDEEEAVAE TKQAPEEEEEREEEQLNRTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFS LRTIRGQQLLEEVTQGDMNAADTFLSDLPRDDIYVSDAEDDDDTSLESDLDPEELAGVRTHSDLKEQKYL RFTKVDDNKEEGGENPLLVPLEEKAVLQEEQASLWFSKDGFSGIEDDADEALEISQAQLLYKSRRKEQQP TDPPPPPTNLKTEKKSPQGQNEVPKETEAILGTEAVTDPGGEERGNSSDSDSSSSEDEDSWKVSRGVKRG RGSKADEDGFEVVPIQDPVKYRILDPEGLALGAVIASSKKAKRDLIDNSFNRYAFNEEEGELPEWFAQEE KQHRIRQLPVDKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKKLEQTKKKAEAVVNTVDISEREKV AQLASPVHTAGLGIAVIVRVFHLAFKGVNHNNLPGCKPKGHAKIVVARMKKDQRAQQRKEQKKKHKRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC012281 |
| ORF Size | 2514 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC012281, AAH12281 |
| RefSeq Size | 2883 bp |
| RefSeq ORF | 2516 bp |
| Locus ID | 56095 |
| MW | 95.2 kDa |
| Gene Summary | RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201000 | Ftsj3 (untagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961), (10ug) |
CNY 6,144.00 |
|
| MG210907 | Ftsj3 (tGFP-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961) |
CNY 9,220.00 |
|
| MR210907L3 | Lenti ORF clone of Ftsj3 (Myc-DDK-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961) |
CNY 10,260.00 |
|
| MR210907L4 | Lenti ORF clone of Ftsj3 (mGFP-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961) |
CNY 10,260.00 |
