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Grik1 (BC031822) Mouse Tagged ORF Clone
CAT＃: MR210866
- TrueORF®
Grik1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (cDNA clone MGC:25101 IMAGE:4501004)
ORF Plasmid: tGFP
"BC031822" in other vectors (4)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC25101 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210866 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCCGAA GAGCATGCGACCAACTGGCTCTTGGGGTGGCCGCCCTCTTCGGTCCTTCCCACAGCTCCTCCGTCAGTGC TGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCTGTGGAC AACAGAGACTTATTTTACATCAACCTCTACCCAGATTATGCAGCTATCAGCAGGGCAGTCCTGGATCTGG TCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTACGAAGACAGCACAGGTCTAATTCGTCTGCAAGA GCTCATCAAAGCTCCCTCCAGATACAACATCAAAATCAAAATCCGCCAGCTTCCCTCTGGCAATAAGGAT GCCAAACCTCTGCTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCGCACGAGA CGGCTGCTGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATACTATCACTACTTCTTCAC AACCCTGGACTTGTTTGCTTTGGATCTGGAACTCTACAGGTACAGTGGTGTAAATATGACTGGATTTCGG TTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGATTGCAGGCCC CACCCAGACCTGAGACTGGTCTCCTGGACGGCGTGATGACAACTGAAGCAGCTCTGATGTACGATGCTGT GTACATGGTAGCCATCGCCTCTCACCGTGCCTCTCAGCTGACCGTCAGTTCCCTGCAGTGCCATCGACAT AAGCCATGGCGCCTAGGACCCAGATTTATGAACCTCATCAAAGAGGCGCGGTGGGATGGCTTGACGGGGC GGATCACCTTCAATAAGACGGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGAGGA AGGAACTGAAAAGATTGGGATTTGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGACAGG TCCAACAATATCACAGATTCGCTGGCTAACCGAACGCTCATTGTCACCACTATTCTGGAAGAGCCCTACG TGATGTACAGGAAATCCGATAAACCACTGTACGGAAATGACAGATTTGAAGGATATTGCCTGGATCTGCT GAAAGAACTGTCAAATATCCTAGGTTTCCTTTATGATGTTAAACTGGTTCCTGACGGCAAATATGGAGCC CAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAGGAACTCATCGACCACAGAGCTGACCTAGCAGTGG CCCCTCTCACCATCACGTATGTACGGGAGAAAGTCATTGACTTCTCCAAGCCTTTCATGACTCTGGGCAT TAGCATCCTTTACCGGAAGCCCAATGGAACCAACCCCGGCGTCTTCTCCTTCCTCAACCCCCTGTCTCCA GACATTTGGATGTATGTGCTCCTCGCTTGCCTAGGAGTCAGTTGTGTGCTTTTTGTGATTGCAAGGTTCA CACCCTACGAGTGGTATAACCCCCACCCGTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTCACTTT GCTAAATAGTTTCTGGTTTGGCGTTGGAGCTCTCATGCGGCAAGGATCGGAGCTGATGCCCAAGGCTCTA TCGACCAGAATAGTTGGAGGAATATGGTGGTTTTTCACCCTAATCATCATCTCATCCTACACTGCCAACC TGGCTGCCTTCTTGACAGTAGAAAGGATGGAATCCCCCATCGATTCCGCAGACGACCTGGCCAAACAAAC CAAGATAGAATACGGGGCAGTCAGAGATGGCTCGACAATGACCTTCTTCAAGAAATCAAAAATCTCCACG TATGAGAAAATGTGGGCTTTCATGAGCAGTAGACAGCAGAGCGCCCTGGTTAAAAACAGCGATGAGGGGA TCCAAAGGGTGCTTACCACCGACTACGCCCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACACAGAG GAACTGCAACCTCACTCAGATCGGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACACCTATCGGC TCCCCTTACCGGGATAAAATTACAATTGCTATTCTTCAACTACAAGAAGAAGGGAAGCTTCATATGATGA AAGAGAAATGGTGGAGGGGAAATGGCTGCCCTGAAGAAGACAGTAAAGAAGCCAGTGCTCTAGGAGTGGA AAATATCGGGGGTATCTTCATTGTTCTGGCTGCAGGACTCGTCCTTTCTGTGTTTGTAGCCATTGGAGAA TTCATATACAAATCACGGAAGAACAATGACATTGAGCAGGCTTTTTGTTTCTTTTATGGACTGCAGTGTA AACAAACCCATCCGACCAACTCCACTTCCGGGACTACATTATCTACAGATGTAGAGTGTGGCAAATTATT ACGAGAGGAAAAGGGGATTCGGACACAGCCCTCAGTCCATACTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210866 protein sequence
Red=Cloning site Green=Tags(s) MPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSVD NRDLFYINLYPDYAAISRAVLDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPSGNKD AKPLLKEMKKGKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVNMTGFR LLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGVMTTEAALMYDAVYMVAIASHRASQLTVSSLQCHRH KPWRLGPRFMNLIKEARWDGLTGRITFNKTDGLRKDFDLDIISLKEEGTEKIGIWNSNSGLNMTDGNRDR SNNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFLYDVKLVPDGKYGA QNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSP DIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMRQGSELMPKAL STRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKIST YEKMWAFMSSRQQSALVKNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVGTPIG SPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDSKEASALGVENIGGIFIVLAAGLVLSVFVAIGE FIYKSRKNNDIEQAFCFFYGLQCKQTHPTNSTSGTTLSTDVECGKLLREEKGIRTQPSVHTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031822 |
| ORF Size | 2496 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031822, AAH31822 |
| RefSeq Size | 3020 bp |
| RefSeq ORF | 2498 bp |
| Locus ID | 14805 |
| MW | 94.1 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220723 | Grik1 (untagged) - Mouse glutamate receptor, ionotropic, kainate 1 (cDNA clone MGC:25101 IMAGE:4501004), (10ug) |
CNY 9,120.00 |
|
| MG210866 | Grik1 (tGFP-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (cDNA clone MGC:25101 IMAGE:4501004) |
CNY 9,120.00 |
|
| MR210866L3 | Lenti ORF clone of Grik1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (cDNA clone MGC:25101 IMAGE:4501004) |
CNY 10,170.00 |
|
| MR210866L4 | Lenti ORF clone of Grik1 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (cDNA clone MGC:25101 IMAGE:4501004) |
CNY 10,170.00 |
