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Vcp (BC043053) Mouse Tagged ORF Clone
CAT＃: MR210758
- TrueORF®
Vcp (Myc-DDK-tagged) - Mouse valosin containing protein (cDNA clone MGC:57954 IMAGE:6418662)
ORF Plasmid: tGFP
"BC043053" in other vectors (3)
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CNY 5,904.00
CNY 8,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p97, CDC48, 3110001E05 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210758 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTGGAGCCGATTCAAAAGGTGATGATTTATCAACAGCCATTCTCAAACAGAAGAACCGACCCA ATCGGTTAATTGTTGATGAAGCCATCAATGAAGATAACAGCGTGGTGTCCTTGTCCCAGCCCAAGATGGA TGAACTGCAGTTGTTCCGAGGTGACACGGTGTTGCTAAAAGGAAAGAAAAGACGGGAAGCTGTATGCATT GTTCTTTCTGATGACACGTGTTCTGATGAGAAGATTCGAATGAATAGAGTTGTTCGGAATAACCTCCGAG TTCGCCTAGGAGATGTCATCAGCATCCAGCCATGCCCTGATGTAAAGTATGGCAAACGTATCCACGTTCT ACCCATCGATGACACAGTGGAAGGCATCACTGGCAATCTCTTTGAGGTATACCTTAAGCCGTACTTCCTG GAAGCTTATCGGCCCATCCGTAAAGGAGATATTTTTCTTGTCCGGGGTGGGATGCGTGCTGTGGAGTTCA AAGTTGTAGAGACAGATCCCAGCCCTTACTGTATTGTTGCTCCAGACACAGTGATCCACTGTGAGGGGGA GCCAATCAAGCGAGAGGATGAGGAGGAATCCTTGAATGAAGTAGGCTATGATGACATCGGTGGTTGCAGG AAGCAGCTAGCTCAGATAAAGGAGATGGTGGAGCTGCCACTGAGACATCCTGCGCTCTTTAAGGCGATTG GTGTAAAGCCTCCTCGGGGAATCTTGTTGTATGGGCCTCCTGGGACAGGGAAGACCCTGATTGCTCGAGC TGTGGCAAATGAAACTGGAGCCTTCTTCTTTCTGATCAATGGTCCTGAAATCATGAGCAAATTGGCTGGT GAGTCTGAGAGCAACCTTCGTAAAGCCTTTGAGGAAGCTGAAAAGAATGCTCCTGCTATCATCTTCATCG ATGAGCTTGATGCCATTGCACCCAAAAGAGAGAAAACTCATGGGGAAGTGGAGCGTCGCATCGTGTCTCA GTTGTTGACCCTCATGGATGGCCTAAAGCAGAGAGCACATGTGATAGTTATGGCAGCAACCAATAGACCC AACAGCATTGACCCAGCCCTACGGCGATTTGGTCGCTTTGACAGAGAGGTAGATATTGGAATACCTGATG CTACAGGACGTTTGGAGATTCTTCAGATCCATACCAAGAACATGAAACTGGCAGATGATGTGGACTTGGA ACAGGTAGCCAATGAGACTCATGGTCATGTTGGTGCTGATTTGGCAGCCCTATGTTCAGAGGCTGCTCTG CAGGCCATCCGGAAAAAAATGGACCTCATTGACCTAGAAGATGAGACCATTGATGCTGAGGTCATGAATT CCCTGGCAGTTACTATGGATGACTTCCGGTGGGCTTTGAGTCAAAGCAACCCATCAGCACTTCGGGAAAC TGTGGTAGAGGTGCCACAAGTAACCTGGGAAGATATTGGAGGCCTGGAGGATGTCAAACGTGAGCTTCAG GAGTTGGTTCAGTATCCTGTGGAACATCCAGACAAATTCCTCAAATTTGGCATGACTCCCTCCAAAGGCG TTCTTTTCTATGGACCTCCTGGCTGTGGGAAAACCTTACTGGCTAAAGCCATTGCTAATGAATGCCAGGC CAACTTCATCTCCATCAAGGGTCCTGAGCTGCTTACCATGTGGTTTGGGGAATCTGAGGCCAATGTCCGG GAAATTTTTGACAAGGCACGGCAAGCTGCCCCCTGTGTACTCTTCTTTGATGAGTTAGATTCAATTGCCA AGGCTCGAGGTGGTAATATTGGAGATGGTGGTGGAGCTGCTGACCGAGTCATCAATCAGATCCTGACAGA AATGGATGGCATGTCTACAAAAAAGAATGTGTTTATCATTGGAGCTACCAACAGGCCTGACATCATTGAT CCTGCTATCCTAAGACCTGGCCGTCTAGATCAGCTCATTTATATCCCACTTCCTGATGAGAAGTCCCGTG TTGCCATCCTAAAAGCCAATCTGCGAAAGTCCCCAGTTGCCAAGGATGTGGATTTGGAGTTCCTGGCTAA GATGACTAATGGCTTTTCTGGAGCTGATTTGACAGAAATTTGCCAACGGGCTTGTAAACTGGCCATTCGT GAATCTATTGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAATCCATCGGCTATGGAGGTAGAAG AGGACGATCCAGTGCCTGAGATCCGCAGAGATCACTTTGAGGAAGCCATGCGTTTTGCCCGACGTTCTGT CAGCGATAATGACATTCGGAAGTATGAAATGTTCGCCCAGACACTGCAGCAGAGTCGAGGTTTTGGCAGC TTCAGATTCCCTTCAGGGAACCAGGGTGGAGCTGGTCCCAGCCAGGGCAGTGGAGGTGGCACAGGTGGCA GTGTGTACACAGAAGACAATGACGATGACCTGTATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210758 protein sequence
Red=Cloning site Green=Tags(s) MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCI VLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFL EAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCR KQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAG ESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRP NSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAAL QAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQ ELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVR EIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIID PAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIR ESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGS FRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC043053 |
| ORF Size | 2418 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC043053.1 |
| RefSeq Size | 2898 bp |
| RefSeq ORF | 2420 bp |
| Locus ID | 269523 |
| MW | 89.3 kDa |
| Gene Summary | Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Plays a role in the regulation of stress granules (SGs) clearance process upon arsenite-induced response (By similarity). Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with DDX58/RIG-I: interaction takes place when DDX58/RIG-I is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58/RIG-I. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210758 | Vcp (tGFP-tagged) - Mouse valosin containing protein (cDNA clone MGC:57954 IMAGE:6418662) |
CNY 8,930.00 |
|
| MR210758L3 | Lenti ORF clone of Vcp (Myc-DDK-tagged) - Mouse valosin containing protein (cDNA clone MGC:57954 IMAGE:6418662) |
CNY 9,980.00 |
|
| MR210758L4 | Lenti ORF clone of Vcp (mGFP-tagged) - Mouse valosin containing protein (cDNA clone MGC:57954 IMAGE:6418662) |
CNY 9,980.00 |
