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Nfx1 (BC053709) Mouse Tagged ORF Clone
CAT＃: MR210754
- TrueORF®
Nfx1 (Myc-DDK-tagged) - Mouse nuclear transcription factor, X-box binding 1 (cDNA clone MGC:60455 IMAGE:30052746)
ORF Plasmid: tGFP
"BC053709" in other vectors (4)
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CNY 5,888.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NFX.1, TEG-42 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210754 representing BC053709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGCCTCCGGTCTCAGGCACTTTTAAATTCAATACAGATGCTGCTGAGTTCATTCCTCAGG AGAGAAAAACTTCTGGTCTAAATTGTGGGACCCAAAGAAGACTAGATTCTAGTAGGATTGGCAGAAGAAA TTATAGTTCCTCGCCTCCCTGTCACCTTCCTAGACACATCCCTTATGAAGACATCTCTGCTGTTCATCAG CACAGTTATGCCTCTGGAAGCAAACCTAAGAGTCCCCAGGGTTTTTTCCAGTCATCTAATAAATCACTCA AGAACCATGGCCTTCAGAATCAGCCATGGCAGAAAGCAAGGAATGAAAAGCACCAAAACAGAAACAAGAA AGCACAAGGGCTCTCTGAGCAGACGTCAGATACATCTAGTTTAGAAAGTGTAGCCAGGTCAGAGAGTGGA ACAAACCCGAGAGAGCACAGCCCTTCCGAGAGTGAGAAGGAGGTGGTTATCGCAGATCCCAGGGGAGCGA AACCCAAAAAAGCAGCACAGCTTACATACAACTATGGCAGAGGACCAAAGGCCAAAGGGAGACTCAGAAG CGAGTGGGGTAATAGAATGAGCCCAAAGTCTGAGGACGAAAATACCAGACCCGTGGCGATTTCCCACACT GACTCTTCAGATGCCTCCTGTAGAAAACCTGTAGTGGATCCATGTGTGTGCAGGCGGAATGAGCAGAGAA GATATCCCCAGAAAAGGCCTCCCTGGGAAGTAGAAGGGGCCAGACCACGGCCAGGCAGGAACCCACCAAA ACAGGAGAGTCAGCGGCATATAAATGCAGGGCCCAAAACCAACATGCCCCCCATTCCAAAGGATAATCTT AGAGAAAGACCAACGAAGTCAGCCTGTGACACTGGGAATTTGGCAGTTGTCAGCAAGTCTTCGAGAAGGG TTAACCAAGAGAAAACTGCTGTAAGGAGACAGGATCCCCAAGTGCTATCTCCTTTTCCCAGAGGCAAACA GAACCATATGCTGAAGAATGTGGAAACACATACAGGTTCCCTGATTGAGCAGCTAACTACAGAAAAATAC GAGTGTATGGTATGCTGTGAGTTGGTTCAGGTCACAGCCCCGGTGTGGAGCTGTCAGAGCTGTTTTCATG TCTTTCATCTGAACTGCATCAAGAAGTGGGCACGCTCTCCAGCATCGCATGCAGATGGCCAGAGTGGTTG GAGGTGCCCTGCTTGTCAGAATGTTTCTGCACATGTTCCCAATACCTATACTTGTTTCTGTGGCAAAGTA AAGAATCCTGAATGGAGCAGAAATGAAATTCCACATAGTTGTGGTGAGGTTTGTAGAAAGAAACAGCCTG GCCAGGACTGTCCACATTCCTGTAACCTTCTCTGCCATCCTGGACCCTGCCCACCCTGCCCTGCTTTTAC AACTAAAACATGTGAATGTGGACGGACCAGGCACACGGTTCGCTGTGGTCAGCCTGTCTCAGTCCACTGT TCTAATGCATGTGAGAATATCTTGAACTGTGGTCAGCACCACTGTGCTGAGCTGTGCCATGGGGGTCAAT GCCAGCCTTGTCGGATCATACTGAACCAGGTGTGCTACTGTGGCAGCACCTCCCGAGATGTGTTGTGTGG AACCGATGTGGGAAAGTCTGATGGATTTGGGGACTTCAGCTGTTTAAAGATATGTGGCAAGGACTTGAAG TGTGGGAGCCATACCTGTTCTCAAGTTTGCCACCCTCAGCCGTGTCAGCCTTGCCCACGGCTCCCCCATT TGGTGCGATACTGCCCTCGCGGCCAAACACCTCTCAGCCAGTTGCTGGAACATGGAAGTAATGCTCGGAA AACATGCATGGATCCTGTCCCTTCCTGTGGAAAAGTGTGTGGCAAACCCTTGGCCTGTGGTTCCTCAGAT TTCATCCACACCTGTGAAAAGCTTTGCCATGAAGGAGACTGTGGGCCGTGCTCTCGCACATCAGTCATTT CCTGCAGATGCTCCTTCAGAACAAAGGAACTTCCGTGTACCAGTCTTAAAAGTGAAGATGCTACATTTAT GTGCGACAAACGGTGTAACAAGAAACGATTGTGCGGACGGCATAAATGTAACGAGATCTGCTGTGTGGAC AAGGAGCACAAGTGTCCTTTGATTTGTGGGAGGAAACTCCGCTGTGGCCTTCATCGGTGTGAAGAACCTT GTCATCGAGGGAACTGTCAGACCTGCTGGCAAGCCAGCTTTGATGAATTAACCTGCCACTGTGGCGCCTC TGTGATCTACCCACCAGTTCCCTGTGGGACTAGGCCTCCTGAGTGTACCCAAACCTGTGCCAGGATCCAT GAATGTGACCATCCAGTATATCATTCTTGTCATAGCGAGGAGAAGTGTCCCCCTTGTACTTTCCTAACTC AGAAGTGGTGCATGGGCAAGCATGAGAGACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210754 representing BC053709
Red=Cloning site Green=Tags(s) MAEAPPVSGTFKFNTDAAEFIPQERKTSGLNCGTQRRLDSSRIGRRNYSSSPPCHLPRHIPYEDISAVHQ HSYASGSKPKSPQGFFQSSNKSLKNHGLQNQPWQKARNEKHQNRNKKAQGLSEQTSDTSSLESVARSESG TNPREHSPSESEKEVVIADPRGAKPKKAAQLTYNYGRGPKAKGRLRSEWGNRMSPKSEDENTRPVAISHT DSSDASCRKPVVDPCVCRRNEQRRYPQKRPPWEVEGARPRPGRNPPKQESQRHINAGPKTNMPPIPKDNL RERPTKSACDTGNLAVVSKSSRRVNQEKTAVRRQDPQVLSPFPRGKQNHMLKNVETHTGSLIEQLTTEKY ECMVCCELVQVTAPVWSCQSCFHVFHLNCIKKWARSPASHADGQSGWRCPACQNVSAHVPNTYTCFCGKV KNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFTTKTCECGRTRHTVRCGQPVSVHC SNACENILNCGQHHCAELCHGGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLK CGSHTCSQVCHPQPCQPCPRLPHLVRYCPRGQTPLSQLLEHGSNARKTCMDPVPSCGKVCGKPLACGSSD FIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVD KEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARIH ECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHERQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053709 |
| ORF Size | 2412 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053709.1 |
| RefSeq Size | 3534 bp |
| RefSeq ORF | 2414 bp |
| Locus ID | 74164 |
| MW | 129.6 kDa |
| Gene Summary | Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220838 | Nfx1 (untagged) - Mouse nuclear transcription factor, X-box binding 1 (cDNA clone MGC:60455 IMAGE:30052746), (10ug) |
CNY 9,120.00 |
|
| MG210754 | Nfx1 (tGFP-tagged) - Mouse nuclear transcription factor, X-box binding 1 (cDNA clone MGC:60455 IMAGE:30052746) |
CNY 5,040.00 |
|
| MR210754L3 | Lenti ORF clone of Nfx1 (Myc-DDK-tagged) - Mouse nuclear transcription factor, X-box binding 1 (cDNA clone MGC:60455 IMAGE:30052746) |
CNY 6,460.00 |
|
| MR210754L4 | Lenti ORF clone of Nfx1 (mGFP-tagged) - Mouse nuclear transcription factor, X-box binding 1 (cDNA clone MGC:60455 IMAGE:30052746) |
CNY 6,460.00 |
