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Pi4kb (NM_175356) Mouse Tagged ORF Clone
CAT＃: MR210726
- TrueORF®
Pi4kb (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase, catalytic, beta polypeptide (Pi4kb)
ORF Plasmid: tGFP
"NM_175356" in other vectors (4)
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CNY 5,872.00
CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ESTM41; PI4K-beta; PI4Kbeta; Pik4cb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACATGGTAGTGGAGCCTGCCACCCTGAAGCCAACTTCTGAGCCTACTCCTAGCCCATCAGGGA ATAATGGGGGCTCCCTACTAAGCGTCATCACGGAGGGGGTCGGGGAACTGTCAGTGATTGACCCTGAGGT GGCCCAGAAGGCCTGCCAGGAGGTACTGGAGAAAGTCAAGCTTTTGCATGGAGGTGTAGCCATCTCTAGC AAAGGCACCCCGCTGGAGTTGGTTAATGGGGATGGTGTGGACAATGAAATCCGTTGCCTAGATGATCCAC CTGCCCAGATCAGGGAGGAAGAAGATGAGATGGGGGCTGGTGTGGCCTCTGGCACAGCCAAAGGAGCAAG ACGACGACGACAGAACAACTCAGCCAAACAGTCTTGGCTCCTGAGGCTGTTTGAGTCAAAACTATTTGAC ATCTCTATGGCTATTTCATACTTGTATAACTCCAAGGAGCCTGGAGTGCAAGCCTACATTGGCAACCGGC TCTTCTACTTTCGCAATGAGGATGTGGACTTCTATTTGCCCCAGTTGCTTAACATGTATATCCACATGGA TGAGGATGTGGGCGATGCCATTAAGCCCTACATAGTCCACCGCTGTCGCCAGAGCATCAACTTTTCCCTC CAGTGTGCCCTGTTGCTTGGGGCCTACTCTTCAGACATGCACATTTCCACTCAGCGACACTCGCGAGGGA CCAAGTTACGGAAGCTAATCCTCTCAGATGAGCTGAAGCCAGCTCACCGAAAGAGGGAGCTGCCGACATT AAGCCCAGCCCCTGACACAGGGCTGTCTCCCTCTAAAAGGACTCACCAGCGTTCTAAGTCAGATGCTACG GCCAGCATAAGTCTCAGCAGCAACCTGAAACGAACAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGC CTGTCCGGCTGGCCCCTGAGCGAGAATTCATCAAGTCTTTGATGGCAATCGGCAAACGGCTGGCCACGCT CCCCACTAAAGAGCAAAAAACACAAAGGCTGATCTCAGAGCTTTCCCTGCTCAACCATAAGCTCCCTGCC CGAGTCTGGCTGCCCACGGCTGGCTTTGACCACCACGTGGTCCGTGTGCCCCACACACAAGCTGTTGTTC TCAATTCCAAGGATAAGGCTCCCTACCTGATCTACGTGGAAGTTCTCGAATGTGAAAACTTTGACACAAC TAGTGTTCCTGCCCGGATTCCTGAAAACCGAATTCGGAGTACACGGTCTGTAGAGAACCTGCCTGAATGT GGTATCACTCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAATTATGACAATGATGATGAAGCCT GGTCAGTGGATGATATAGGCGAGCTGCAAGTGGAGCTCCCTGAAGTGCACACCAACAGCTGTGACAACAT CTCCCAGTTCTCGGTGGACAGCATCACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTGCAGCAGGGGAC ATCCGACGGCGCCTTTCAGAACAGCTGGCTCATACTCCTACAGCCTTCAAACGAGACCCTGAAGACCCTT CTGCAGTTGCCCTCAAAGAGCCCTGGCAGGAGAAAGTGCGGAGGATCAGAGAAGGTTCCCCGTATGGCCA TCTTCCCAATTGGCGACTCCTTTCAGTCATTGTCAAGTGTGGAGATGACCTTCGCCAGGAGCTGCTGGCT TTCCAGGTGTTGAAACAACTGCAGTCCATTTGGGAACAGGAGCGAGTGCCTCTTTGGATCAAGCCATATA AGATTCTTGTGATTTCAGCTGACAGTGGCATGATTGAACCAGTAGTCAACGCTGTGTCCATCCACCAGGT GAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAACATGGCAGTTATACCACTGAGGCA TTCCTCAGTGCCCAGCGCAATTTTGTGCAAAGCTGTGCTGGCTACTGCTTGGTCTGCTACCTATTGCAAG TCAAGGACAGGCACAACGGGAACATCCTTCTGGACGCAGAAGGTCACATCATCCACATCGACTTTGGCTT CATCCTTTCCAGCTCACCCCGAAACCTGGGCTTCGAGACATCAGCCTTTAAGCTGACCACAGAATTTGTG GATGTAATGGGTGGCCTGAACGGTGATATGTTCAACTACTACAAGATGCTCATGCTGCAAGGGCTGATTG CTGCTCGGAAGCACATGGACAAGGTGGTACAGATTGTGGAGATCATGCAGCAAGGTTCTCAGCTTCCTTG CTTCCATGGCTCCAGCACCATTCGCAACCTCAAAGAGAGGTTCCACATGAGCATGACTGAGGAGCAGCTG CAGCTGCTGGTGGAGCAGATGGTGGACGGCAGCATGAGGTCCATCACCACCAAACTCTACGATGGCTTCC AGTACCTCACCAATGGCATCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210726 protein sequence
Red=Cloning site Green=Tags(s) MGDMVVEPATLKPTSEPTPSPSGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAISS KGTPLELVNGDGVDNEIRCLDDPPAQIREEEDEMGAGVASGTAKGARRRRQNNSAKQSWLLRLFESKLFD ISMAISYLYNSKEPGVQAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSL QCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPTLSPAPDTGLSPSKRTHQRSKSDAT ASISLSSNLKRTASNPKVENEDEPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPA RVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPEC GITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGD IRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLA FQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEA FLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFV DVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQL QLLVEQMVDGSMRSITTKLYDGFQYLTNGIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175356 |
| ORF Size | 2406 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175356.3, NP_780565.2 |
| RefSeq Size | 3786 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 107650 |
| UniProt ID | Q8BKC8 |
| MW | 89.9 kDa |
| Gene Summary | Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221725 | Pi4kb (untagged) - Mouse phosphatidylinositol 4-kinase, catalytic, beta polypeptide (Pi4kb), (10ug) |
CNY 10,260.00 |
|
| MG210726 | Pi4kb (tGFP-tagged) - Mouse phosphatidylinositol 4-kinase, catalytic, beta polypeptide (Pik4cb) |
CNY 7,700.00 |
|
| MR210726L3 | Lenti ORF clone of Pi4kb (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase, catalytic, beta polypeptide (Pi4kb) |
CNY 8,930.00 |
|
| MR210726L4 | Lenti ORF clone of Pi4kb (mGFP-tagged) - Mouse phosphatidylinositol 4-kinase, catalytic, beta polypeptide (Pi4kb) |
CNY 8,930.00 |
