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Atxn1 (NM_009124) Mouse Tagged ORF Clone
CAT＃: MR210675
- TrueORF®
Atxn1 (Myc-DDK-tagged) - Mouse ataxin 1 (Atxn1)
ORF Plasmid: tGFP
"NM_009124" in other vectors (4)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2900016G23Rik; Atx1; C85907; ENSMUSG00000074917; Gm10786; Sca1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210675 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAAGAAACGTGAGATCCCCGCCACCAGCC GGCCCTCGGAGGAGAAGGCCACTGCTCTGCCCAGCGACAACCACTGCGTGGAGGGTGTGGCCTGGCTCCC CAGCACCCCTGGCATCCGCGGCCATGGGGGTGGGCGGCACGGGTCAGCAGGGACTTCCGGGGAGCATGGT TTACAAGGAATGGGTTTACATAAAGCACTGTCCGCAGGGCTGGATTACTCCCCACCCAGTGCCCCCAGGT CAGTCCCCACAGCCAACACGCTGCCCACCGTGTACCCTCCTCCTCAGTCAGGGACCCCGGTGTCTCCTGT GCAGTACGCCCACCTTTCGCATACCTTCCAGTTCATTGGGTCCTCCCAATACAGTGGGCCTTACGCGGGC TTTATCCCTTCCCAGCTGATCTCCCCATCAGGCAACCCGGTCACCAGTGCAGTAGCCTCAGCTGCAGGGG CCACCACTCCATCACAGCGCTCCCAGCTGGAGGCTTATTCCACCCTGCTGGCCAACATGGGCAGTCTGAG CCAGGCACCAGGACATAAGGTTGAGCCCCCTCCGCAGCAGCACCTCAGCAGGGCTGCAGGATTAGTCAAC CCGGGGTCCCCTCCTCCACCCACCCAGCAGAACCAGTACATCCATATTTCCAGCTCTCCACAGAGCTCCG GGCGGGCGACATCTCCCCCACCCATCCCGGTCCACCTCCATCCCCATCAGACGATGATCCCGCACACACT CACCCTGGGGCCTTCATCCCAGGTGGTTGTGCAATATAGTGATGCCGGAGGCCACTTTGTTCCTCGAGAG TCCACCAAAAAAGCCGAGAGCAGCAGGTTGCAGCAGGCTATGCAAGCCAAGGAAGTCCTGAATGGGGAGA TGGAGAAAAGCCGGAGGTATGGGGCATCATCTTCTGTGGAGCTGAGCCTAGGCAAGGCAAGCAGTAAGTC AGTGCCTCATCCCTATGAGTCCAGGCATGTGGTGGTCCACCCAAGCCCAGCAGACTACAGCAGTCGTGAT ACCTCCGGGGTCCGTGGATCTGTGATGGTTCTGCCTAATAGCAGCACACCCTCAGCCGACCTGGAGGCCC AGCAGACCACGCATCGAGAGGCCTCCCCATCCACCCTCAATGACAAGAGCGGCCTGCACCTAGGGAAGCC GGGCCACAGGTCTTATGCGCTGTCCCCCCACACGGTCATTCAGACCACACACAGTGCATCAGAGCCTCTC CCGGTGGGCCTACCAGCCACGGCCTTCTACGCTGGCACTCAACCTCCTGTCATCGGCTACCTGAGCGGCC AGCAGCAAGCAATCACCTATGCTGGTGGTCTGCCGCAGCACCTGGTGATCCCAGGTAACCAGCCCCTGCT CATCCCGGTGGGCAGCCCTGACATGGACACGCCTGGGGCAGCCTCGGCCATCGTGACGTCATCACCCCAG TTTGCTGCAGTACCTCACACGTTTGTCACCACCGCCCTGCCCAAGAGCGAGAACTTCAACCCAGAGGCTC TGGTCACCCAGGCTGCCTACCCAGCCATGGTGCAGGCCCAGATCCACCTGCCGGTGGTGCAGTCCGTGGC GTCCCCCACCACGGCGTCTCCCACGCTGCCGCCATATTTCATGAAAGGCTCCATCATCCAGCTGGCCAAC GGGGAGCTGAAGAAGGTGGAGGACCTGAAGACGGAGGATTTCATCCAGAGTGCAGAGATTAGCAATGACC TCAAGATCGACTCCAGTACTGTGGAGAGAATCGAGGAGAGCCACAGCCCCGGGGTGGCCGTGATACAGTT TGCTGTTGGTGAACACCGAGCCCAGGTCAGTGTCGAAGTCTTGGTAGAGTATCCTTTTTTTGTATTTGGA CAGGGCTGGTCATCCTGCTGTCCTGAGCGGACCAGCCAGCTCTTTGATCTGCCGTGTTCCAAACTCTCTG TTGGGGACGTCTGCATCTCGCTCACCCTCAAGAACCTGAAGAATGGCTCTGTTAAAAAGGGCCAGCCTGT GGACCCTGCCAGCGTCCTGCTGAAGCAGGCAAAGACCGACAGCCTGGCTGGCAGCAGACACAGATACGCG GAGCAGGAAAACGGAATCAACCAGGGAAGCGCCCAGGTGCTCTCTGAGAATGGCGAACTGAAGTTTCCAG AAAAAATAGGATTGCCTGCAGCACCCTTCCTCAGCAAAATAGAACCGAGCAAACCCACAGCCACGAGGAA GAGGAGGTGGTCGGCGCCGGAGACCCGTAAACTGGAGAAGTCGGAGGACGAGCCACCTTTGACTCTTCCC AAGCCTTCGCTCATTCCTCAGGAGGTTAAGATCTGCATCGAAGGCCGATCTAACGTGGGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210675 protein sequence
Red=Cloning site Green=Tags(s) MKSNQERSNECLPPKKREIPATSRPSEEKATALPSDNHCVEGVAWLPSTPGIRGHGGGRHGSAGTSGEHG LQGMGLHKALSAGLDYSPPSAPRSVPTANTLPTVYPPPQSGTPVSPVQYAHLSHTFQFIGSSQYSGPYAG FIPSQLISPSGNPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPPPQQHLSRAAGLVN PGSPPPPTQQNQYIHISSSPQSSGRATSPPPIPVHLHPHQTMIPHTLTLGPSSQVVVQYSDAGGHFVPRE STKKAESSRLQQAMQAKEVLNGEMEKSRRYGASSSVELSLGKASSKSVPHPYESRHVVVHPSPADYSSRD TSGVRGSVMVLPNSSTPSADLEAQQTTHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPL PVGLPATAFYAGTQPPVIGYLSGQQQAITYAGGLPQHLVIPGNQPLLIPVGSPDMDTPGAASAIVTSSPQ FAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPTTASPTLPPYFMKGSIIQLAN GELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEESHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFG QGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKQAKTDSLAGSRHRYA EQENGINQGSAQVLSENGELKFPEKIGLPAAPFLSKIEPSKPTATRKRRWSAPETRKLEKSEDEPPLTLP KPSLIPQEVKICIEGRSNVGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009124 |
| ORF Size | 2376 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009124.6 |
| RefSeq Size | 10599 bp |
| RefSeq ORF | 2376 bp |
| Locus ID | 20238 |
| UniProt ID | P54254 |
| MW | 83.8 kDa |
| Gene Summary | Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression (By similarity). May be involved in RNA metabolism (By similarity). In concert with CIC and ATXN1L, involved in brain development (PubMed:28288114).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202345 | Atxn1 (untagged) - Mouse ataxin 1 (Atxn1), (10ug) |
CNY 2,000.00 |
|
| MG210675 | Atxn1 (tGFP-tagged) - Mouse ataxin 1 (Atxn1) |
CNY 6,370.00 |
|
| MR210675L3 | Lenti ORF clone of Atxn1 (Myc-DDK-tagged) - Mouse ataxin 1 (Atxn1) |
CNY 7,700.00 |
|
| MR210675L4 | Lenti ORF clone of Atxn1 (mGFP-tagged) - Mouse ataxin 1 (Atxn1) |
CNY 7,700.00 |
