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Tarsl2 (NM_172310) Mouse Tagged ORF Clone
CAT＃: MR210670
- TrueORF®
Tarsl2 (Myc-DDK-tagged) - Mouse threonyl-tRNA synthetase-like 2 (Tarsl2)
ORF Plasmid: tGFP
"NM_172310" in other vectors (4)
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CNY 5,784.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A530046H20Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210670 representing NM_172310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCAGGCCCTGGCGGCTCAGGCGGTGGCCTCGCGCCTGCAGCGGCAGGAGGAGGACATCCGCT GGCTGTGCGCGGAGGTGCAGCGCCTGAGGGACGAGCAGCTGCGCGGGCCCGAGCGGGGCCAAGCCGAGGG CCCGCGCCTGACACGCGAGGTGGCACAGCTCCAGGCGGAGAACCGCGACCTGCACCAGCGCCTGTGTGGC CTGCGGCTGCGCCTGGCGGAGCAGCGCCGCACCGAGGCCGGGCGAGCGGCGGCGCACGAGCCTCCTACCC AAAACCAAGAGAAGGACACAAAGAAGAAGAGGCTGAAGCAGAGTGAGCCTGGCCGGGAGGTGAAGCAACC GAATTTTATAAAAGAGAGATTGCAGCTTTTTGAAACATTGAAGACAGATCATCAACTGTTACCTGCTACT CAGGAAAAGAAGAACACAAACAACGTGATTTCAGTGAGGGTGGCTGGTGGGAAGACAGTGCAAGGAGAAA GGTGGAAAACAACACCTTATCAAGTGGCCGCTGGAATTAGTAAGGAATTGGCTGAACATACAGTGATTGC CAAAGTGAACGGTGTATTGTGGGATCTGGACCGCCCGTTGGAAGGGGACTCCACTGTGGAGCTGCTGATG TTTGACAACGAGGAAGCCCAGGCTGTGTACTGGCATTCCAGCGCTCACATCCTGGGGGAGGCCATGGAAC TTTACTATGGAGGCCACCTGTGTTATGGCCCACCCATTGAAAATGGGTTTTATTATGATATGTTTATTGA AGACAGAGTGGTGTCCAGCACTGAGTTGTCTGCCCTGGAGAACATATGTAAAACTATCATCAAAGAAAAG CAACCGTTTGAAAGACTGGAAGTCAGCAAGGACACTCTCCTGGAAATGTTTAAGTACAATAAATTTAAGT GCCGGATTCTGAAGGAGAAAGTGGACACTCCGACGACCACTGTTTACAGGTGTGGTCCATTGATTGACCT TTGTAAAGGCCCCCATGTAAGACACACTGGAAAAATTAAAGCCATAAAAATTTTCAAGAACTCCTCAACA TATTGGGAAGGCAACCCTGAGATGGAAACTTTGCAGCGGATCTATGGGATATCCTTTCCTGACAGCAAAA TGATGAAAGACTGGGAAAAATTCCAAGAGGAAGCCAAGAGCCGGGACCACAGGAAAATCGGGAAGGAACA AGAACTTTTCTTCTTCCATGATCTGAGCCCTGGAAGCTGTTTTTTCCTCCCGAGAGGAGCGTTCATTTAC AATGCGCTTATGGATTTCATACGAGAGGAATATCACAAGCGTAACTTCACAGAAGTGCTCTCTCCCAACA TGTACAACAGTAAACTCTGGGAAACATCAGGCCACTGGCAGCATTACAGTAACAACATGTTCACCTTTGA CGTCGAGAAGGACACTTTCGCCCTCAAACCCATGAACTGTCCAGGGCACTGTTTAATGTTCGCACATCGC CCGCGATCCTGGAGGGAAATGCCTGTTAGATTTGCTGATTTTGGAGTTCTTCACAGAAATGAGCTGTCAG GCACTTTAAGCGGCTTAACCCGAGTGAGGCGCTTCCAGCAAGATGATGCGCACATCTTCTGCATGGTGGA GCAGATCGAAGAAGAAATAAAGGGGTGCTTGCACTTTTTACAGTCCGTTTACTCGACGTTTGGCTTCTCC TTTCAATTAAACCTGTCCACAAGGCCTGAGCACTTCCTAGGAGAGATTGAGATATGGGATGAAGCTGAAA GGCAACTGCAGAACAGCTTGGTGGAATTTGGAAAACCATGGAAAATAAACCCAGGAGATGGAGCATTCTA TGGCCCTAAAATCGATATAAAAATTAAAGATGCTATTGGCAGATACCATCAGTGTGCTACAATTCAGCTG GACTTCCAACTGCCTATCAGGTTTAACCTCACATATGTGAGTAAGGATGGGGATGACAAGAACAGGCCTG TGATCATTCACCGCGCCATCCTGGGGTCTGTGGAAAGGATGATAGCCATCCTCTCAGAGAACTACGGGGG GAAATGGCCTCTCTGGCTGTCCCCTCGCCAGGTAATGGTCATCCCAGTAGGCCCAGCTTGTGAAAATTAT GCACTACAGGTGTCCAAGGAATGCTTCGAAGAAGGCTTCATGGCCGATGTGGACTTAGATGACAGTTGTA CACTAAATAAGAAAATACGGAATGCACAGCTGGCTCAGTATAATTTCATTTTGGTGGTTGGCGAAAAGGA AAAGATCAACAATGCAGTAAATGTTCGAACAAGAGACAACAAAATTCATGGAGAAATATCAATAGCTTCC GTCATTGAAAAATTGAAGAACCTCAAAAAATCACGTACGCTAAATGCCGAGGAAGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210670 representing NM_172310
Red=Cloning site Green=Tags(s) MAAQALAAQAVASRLQRQEEDIRWLCAEVQRLRDEQLRGPERGQAEGPRLTREVAQLQAENRDLHQRLCG LRLRLAEQRRTEAGRAAAHEPPTQNQEKDTKKKRLKQSEPGREVKQPNFIKERLQLFETLKTDHQLLPAT QEKKNTNNVISVRVAGGKTVQGERWKTTPYQVAAGISKELAEHTVIAKVNGVLWDLDRPLEGDSTVELLM FDNEEAQAVYWHSSAHILGEAMELYYGGHLCYGPPIENGFYYDMFIEDRVVSSTELSALENICKTIIKEK QPFERLEVSKDTLLEMFKYNKFKCRILKEKVDTPTTTVYRCGPLIDLCKGPHVRHTGKIKAIKIFKNSST YWEGNPEMETLQRIYGISFPDSKMMKDWEKFQEEAKSRDHRKIGKEQELFFFHDLSPGSCFFLPRGAFIY NALMDFIREEYHKRNFTEVLSPNMYNSKLWETSGHWQHYSNNMFTFDVEKDTFALKPMNCPGHCLMFAHR PRSWREMPVRFADFGVLHRNELSGTLSGLTRVRRFQQDDAHIFCMVEQIEEEIKGCLHFLQSVYSTFGFS FQLNLSTRPEHFLGEIEIWDEAERQLQNSLVEFGKPWKINPGDGAFYGPKIDIKIKDAIGRYHQCATIQL DFQLPIRFNLTYVSKDGDDKNRPVIIHRAILGSVERMIAILSENYGGKWPLWLSPRQVMVIPVGPACENY ALQVSKECFEEGFMADVDLDDSCTLNKKIRNAQLAQYNFILVVGEKEKINNAVNVRTRDNKIHGEISIAS VIEKLKNLKKSRTLNAEEDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172310 |
| ORF Size | 2370 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172310.2, NP_758514.2 |
| RefSeq Size | 3194 bp |
| RefSeq ORF | 2373 bp |
| Locus ID | 272396 |
| UniProt ID | Q8BLY2 |
| MW | 91.3 kDa |
| Gene Summary | Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221668 | Tarsl2 (untagged) - Mouse threonyl-tRNA synthetase-like 2 (Tarsl2), (10ug) |
CNY 10,170.00 |
|
| MG210670 | Tarsl2 (tGFP-tagged) - Mouse threonyl-tRNA synthetase-like 2 (Tarsl2) |
CNY 6,370.00 |
|
| MR210670L3 | Lenti ORF clone of Tarsl2 (Myc-DDK-tagged) - Mouse threonyl-tRNA synthetase-like 2 (Tarsl2) |
CNY 7,700.00 |
|
| MR210670L4 | Lenti ORF clone of Tarsl2 (mGFP-tagged) - Mouse threonyl-tRNA synthetase-like 2 (Tarsl2) |
CNY 7,700.00 |
