Sema3a (NM_009152) Mouse Tagged ORF Clone
CAT#: MR210565
- TrueORF®
Sema3a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a)
"NM_009152" in other vectors (3)
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CNY 5,656.00
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | coll-1; Hsema-I; SEMA1; Semad; SemD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210565 representing NM_009152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTGTATTACTTACAGCCAGAGCAAACTATGCAA ACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAATCCAACAATGTGATCAC TTTTAATGGCTTGGCCAACAGCTCCAGTTACCACACCTTCCTTCTGGATGAAGAACGGAGTAGACTATAT GTTGGAGCAAAAGATCATATATTTTCATTCAACTTGGTGAACATTAAAGATTTTCAAAAGATTGTGTGGC CAGTATCTTACACAAGGAGAGATGAATGCAAATGGGCTGGAAAAGATATCCTGAAAGAATGTGCCAATTT CATCAAGGTCCTGGAGGCTTATAATCAGACTCACTTGTATGCCTGTGGAACTGGGGCTTTCCATCCAATC TGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAGCTGCAGGACTCACATTTTGAAA ACGGTCGTGGGAAGAGCCCTTATGATCCCAAACTACTGACTGCCTCTCTTCTAATAGACGGTGAGTTGTA CTCTGGAACTGCTGCGGACTTCATGGGACGGGACTTCGCTATCTTCAGAACACTGGGGCACCATCACCCC ATCAGGACGGAGCAGCATGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGTGCCCATCTCATCCCAG AGAGTGACAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAATAGACGGAGAACACTC TGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGTGGACACAGAAGTCTTGTG AATAAATGGACAACATTCCTAAAAGCACGCCTGATTTGCTCTGTGCCCGGTCCCAATGGCATTGACACCC ATTTTGATGAATTGCAGGATGTATTCCTAATGAACTCTAAAGATCCTAAAAATCCGATCGTCTATGGAGT GTTCACAACATCAAGCAACATCTTTAAGGGATCTGCTGTGTGCATGTACAGCATGAGTGATGTAAGAAGG GTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTGCCTTACCAAGGAAGAGTCC CTTATCCACGGCCAGGAACTTGTCCCAGTAAAACATTTGGCGGATTTGACTCCACAAAGGACCTTCCTGA TGATGTCATAACTTTTGCAAGAAGTCATCCAGCCATGTACAACCCAGTGTTTCCTATAAATAATCGCCCG ATCATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGAGTGGATGCAGAAGATG GCCAGTATGATGTTATGTTCATCGGAACAGATGTTGGAACCGTTCTTAAAGTGGTTTCAGTCCCCAAGGA GACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACCGTCTTCCGGGAACCAACAACTATTTCG GCAATGGAGCTTTCTACTAAACAGCAACAGCTGTACATTGGCTCAACTGCGGGAGTGGCACAGCTTCCTC TACACCGCTGTGACATCTATGGCAAAGCCTGTGCAGAATGCTGCCTCGCTCGGGACCCTTACTGTGCCTG GGATGGGTCCTCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACGACAAGATATAAGGAAT GGAGACCCACTGACTCACTGCTCTGACTTGCAGCACCATGATAATCATCATGGGCCCAGCCTTGAAGAGA GAATCATCTATGGAGTGGAAAACAGTAGTACATTCTTGGAATGCAGTCCGAAGTCACAGAGAGCCTTGGT ATATTGGCAATTTCAGAGGAGAAATGAAGATCGAAAAGAGGAGATCAGAATGGGTGATCATATCATCAGG ACAGAACAAGGGCTCCTGCTCCGTAGCCTGCAGAAGAAGGATTCAGGCAATTACCTGTGTCACGCTGTGG AACACGGATTCATGCAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAACATTTGGAAGAACT TCTTCATAAAGATGACGATGGAGATGGCTCTAAGATAAAAGAAATGTCGAGCAGCATGACGCCCAGCCAG AAAGTCTGGTACCGAGACTTCATGCAGCTCATTAACCACCCCAACCTGAACACGATGGATGAGTTCTGTG AACAAGTGTGGAAAAGGGACCGAAAGCAACGCCGACAAAGGCCGGGGCACTCTCAAGGGAGCAGCAACAA GTGGAAGCACATGCAAGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTTTGAGCGGGCACCCAGA AGTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210565 representing NM_009152
Red=Cloning site Green=Tags(s) MGWFTGIACLFWGVLLTARANYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY VGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLEAYNQTHLYACGTGAFHPI CTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP IRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRR VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRP IMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTIS AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRN GDPLTHCSDLQHHDNHHGPSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRMGDHIIR TEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKIKEMSSSMTPSQ KVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPR SV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009152 |
ORF Size | 2316 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009152.4 |
RefSeq Size | 5946 bp |
RefSeq ORF | 2319 bp |
Locus ID | 20346 |
UniProt ID | O08665 |
MW | 89.2 kDa |
Gene Summary | Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205153 | Sema3a (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10ug) |
CNY 5,664.00 |
|
MR210565L3 | Lenti ORF clone of Sema3a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a) |
CNY 8,056.00 |
|
MR210565L4 | Lenti ORF clone of Sema3a (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a) |
CNY 7,700.00 |