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Fert2 (BC058100) Mouse Tagged ORF Clone
CAT＃: MR210538
- TrueORF®
Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507)
ORF Plasmid: tGFP
"BC058100" in other vectors (4)
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CNY 5,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AV082135; C330004K01Rik; Fert; Fert2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210538 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATTTGGGAGTGACCTGAAGAACTCACAGGAAGCTGTGTTAAAGTTGCAAGACTGGGAACTACGGT TGCTGGAGACAGTGAAGAAATTTATGGCTCTGAGAATAAAGAGTGATAAAGAATATGCATATACTCTACA GAATCTTTGTAATCAAGTTGATAAAGAAAGCACTGTGCAAGTGAATTATGTCAGCAACGTGTCCAAGGTC ACAAAGACAGAATTGGAGAAATTAAAATCCAGCTATCGACAATTAATAAAAGAAATGAATTCTGCCAAAG AGAAATATAAAGAAGCCTTAGCGAAAGGGAAGGAAACAGAAAAGGCCAAGGAGCGCTATGACAAAGCTAC AATGAAGCTTCATATGCTGCATAATCAGTATGTGTTGGCACTGAAGGGGGCACAGCTCCATCAGAGCCAG TACTATGATACCACACTTCCTCTGCTGCTGGACTCCGTGCAAAAGATGCAAGAAGAAATGATAAAAGCAC TAAAAGGTATATTTGATGACTATAGCCAGATAACCAGCCTTGTTACAGAGGAAATAGTGAATGTCCATAA AGAGATTCAAATGTCTGTTGAACAGATAGACCCTAGCACCGAATATAATAATTTCATAGATGTTCACAGA ACAACAGCTGCTAAAGAACAAGAAATCGAATTTGATACTTCCCTATTAGAAGAAAATGAAAATCTTCAGG CAAATGAGATCATGTGGAATAATTTAACAGCAGACAGTTTGCAAGTCATGTTGAAGACTTTAGCGGAAGA GCTCACACAGACCCAGCAGATGCTTTTACACAAGGAGGCGGCCGTCCTGGAGCTAGAGAAGAGGATTGAA GAATCTTTTGAGACCTGTGAAAAGAAGTCTGACATTGTGCTTCTTCTGGGCCAAAAACAGGCACTTGAAG AGTTGAAACAGTCAGTCCAGCAGTTGCGATGCACTGAGGCAAAGTGTGCAGCACAGAAAGCTTTGCTGGA GCAAAAAGTACAGGAAAATGATGGGAAAGAACCACCTCCTGTGGTGAATTACGAAGAAGACGCGCGGTCA GTCACATCCATGGAAAGAAAGGAGAGGCTATCCAAATTTGAGTCTATTCGTCATTCAATTGCTGGGATAA TTAAGTCTCCAAAGTCGGTACTCGGATCTTCAACACAGGTGTGTGATGTGATCTCTGTGGGTGAGAGGCC CCTGGCGGAGCATGACTGGTACCATGGTGCCATCCCCAGGATAGAGGCACAGGAACTGCTGAAGCAGCAA GGAGACTTCCTGGTGCGGGAGAGCCATGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGACGGAC AAAGGAGGCACTTTATCATACAATTTGTCGATAATCTGTATCGATTCGAGGGCACCGGGTTTTCAAACAT TCCCCAGCTTATAGATCACCACTTCAATACAAAGCAAGTCATCACCAAGAAGTCTGGGGTGGTTCTGCTC AACCCCATCCCAAAGGATAAGAAATGGGTTCTCAATCATGAAGATGTTTCATTGGGAGAATTACTGGGCA AGGGGAATTTTGGTGAAGTGTATAAGGGCACACTAAAGGATAAAACTCCTGTTGCCATTAAAACGTGCAA GGAAGACCTTCCTCAGGAATTAAAAATAAAGTTTCTACAGGAAGCCAAAATTCTGAAGCAATATGATCAT CCCAATATTGTCAAACTGATAGGCGTGTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTCC CAGGGGGTGATTTTCTGACCTTCCTGAGGAAGAGGAAGGACGAGCTGAAGCTGAAGCAGTTGGTGAGATT TTCCTTGGACGTTGCTGCTGGCATGTTGTATCTCGAGAGCAAGAACTGCATTCACAGGGACCTGGCTGCA CGGAACTGCCTGGTGGGTGAAAATAATACTCTGAAAATCAGTGACTTTGGAATGTCTCGGCAAGAAGACG GTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTCCTATTAAATGGACAGCACCGGAAGCTCTTAATTA TGGGAGATACAGTTCTGAAAGTGACGTGTGGAGCTTCGGCATCCTCCTCTGGGAGACCTTCAGCCTAGGA GTCTGCCCGTACCCTGGAATGACAAACCAGCAAGCGCGAGAGCAAGTGGAGAGAGGATACCGGATGTCAG CCCCACAGAACTGTCCAGAGGAGGTTTTTACAATCATGATGAAGTGTTGGGATTACAAGCCTGAAAACCG CCCTAAGTTCAACGACCTTCACAAAGAGCTCACTGTCATCAAGAAGATGATCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210538 protein sequence
Red=Cloning site Green=Tags(s) MGFGSDLKNSQEAVLKLQDWELRLLETVKKFMALRIKSDKEYAYTLQNLCNQVDKESTVQVNYVSNVSKV TKTELEKLKSSYRQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQSQ YYDTTLPLLLDSVQKMQEEMIKALKGIFDDYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHR TTAAKEQEIEFDTSLLEENENLQANEIMWNNLTADSLQVMLKTLAEELTQTQQMLLHKEAAVLELEKRIE ESFETCEKKSDIVLLLGQKQALEELKQSVQQLRCTEAKCAAQKALLEQKVQENDGKEPPPVVNYEEDARS VTSMERKERLSKFESIRHSIAGIIKSPKSVLGSSTQVCDVISVGERPLAEHDWYHGAIPRIEAQELLKQQ GDFLVRESHGKPGEYVLSVYSDGQRRHFIIQFVDNLYRFEGTGFSNIPQLIDHHFNTKQVITKKSGVVLL NPIPKDKKWVLNHEDVSLGELLGKGNFGEVYKGTLKDKTPVAIKTCKEDLPQELKIKFLQEAKILKQYDH PNIVKLIGVCTQRQPVYIIMELVPGGDFLTFLRKRKDELKLKQLVRFSLDVAAGMLYLESKNCIHRDLAA RNCLVGENNTLKISDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLG VCPYPGMTNQQAREQVERGYRMSAPQNCPEEVFTIMMKCWDYKPENRPKFNDLHKELTVIKKMIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058100 |
| ORF Size | 2295 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC058100, AAH58100 |
| RefSeq Size | 2948 bp |
| RefSeq ORF | 2297 bp |
| Locus ID | 14158 |
| MW | 87.8 kDa |
| Gene Summary | Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220721 | Fert2 (untagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507), (10ug) |
CNY 9,120.00 |
|
| MG210538 | Fert2 (tGFP-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507) |
CNY 4,850.00 |
|
| MR210538L3 | Lenti ORF clone of Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507) |
CNY 6,270.00 |
|
| MR210538L4 | Lenti ORF clone of Fert2 (mGFP-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507) |
CNY 6,270.00 |
