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Adam32 (NM_153397) Mouse Tagged ORF Clone
CAT＃: MR210459
- TrueORF®
Adam32 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 32 (Adam32)
ORF Plasmid: tGFP
"NM_153397" in other vectors (4)
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CNY 5,520.00
CNY 6,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210459 representing NM_153397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCGCCATGCTGCACACACTGCTGCTGCTGCTGCTGGCCGAGCTGGGGGCCTTGCTGGCATCAG GACCCGAGTCTCAAAGTTCATTTCTAGAAATCATATTTCCAGAGAAAATTGAAGATAAAACACACTCAGA AGAACAGATATCCTATATTATTCCAATAAACAAGAAGCAGTACACTGTGCACCTCCAGAAAAGATATTTT TTAACGAATCGTTTTATGGTTTATATGTATAATCAAGGATCTACAAGTTTTCATTCTCCAAACATTCCGG CTCAGTGCTATTATCAAGGACACATCAAAGGCTACCCGAACTCAGTCGCCACCCTCAGCACATGCTCTGG ACTGAGAGGGTTCCTGCAGTTTGAGAATGTGTCCTACGGAATTGAGCCTCTGCAATCTGCATTTACATCT CAACACATTGTTTATAAGCTAGGGAACAAAGAGAAGGAGTTGATATTTAACAAAAACAGCAGAAACATAG AGATGCCTACAAACTATGGCATTTTAATCAACAAAAAGCCAAAATCACCTTTTAAAAACTTGTTTCCCCT CTATCTAGAGATGAGTATTGTGGTGGACAAAGCGCTGTATGATTACTTGGGCTCTGACAGCAATATTGTA ACAAATAAAATCATTGAAATTATCAGTCTTATAAATTCAGTGTTTGCCCAACTCAAGGTTACTATCGTGT TGTCCTCATTGGAGTTGTGGTCAGATAAGAATAAGATTCCTACAGTTGGTGAAGCAGATGAATTATTGCA TAAATTTCTAGAATGGAAGCAGGCCTACCTTACCCTAAGACCTCATGATGTTGCATATCTATTCATTTAT AACGAGTACCCAAATTACATGGGAGCAACGTATCCTGGGAAGATGTGTACTGCCCACTACTCTGCAGGAA TCACAATGTACCCCAAGGATATGACCTTGGAGGCCTTTTCGGTTATTCTCACCCAAATGCTGGGGCTCAG TCTGGGGATATCGTATGATGAGCCTGAGAAATGCTACTGCTCGGAATCCATCTGCATCATGAATCCCAGG GCGATGCAATATGGAGGTGTGAAGTCATTTAGCAATTGCAGTTTGAATGACTTTGAACATTTTAAATCGA ACGAGGGTGCCAAATGTCTGCAGAATAAGCCACAGATGCAAAGGACTGCAGCAGCGGTCTGCGGAAATGG CAAGGTGGAGGGTGATGAAATTTGTGACTGTGGCTCTGAAGCAGAATGTGGGCCTGATAGCTGTTGTGAA CCTAACAGATGTGTTCTGAAAGCAGGAAGAGCTTGTGACAGTAAGTCTCCCTCATCAACATGCTGCAAAA ATTGTCAATTTTTACCAGAAAAACATCAATGTAGGCCGGAAAAGCATCTATACTGTGACATTCCTGAAGT CTGCAATGGGAGCTCAGGAAATTGTCCACCTGATGTAACTATAAACAATGGGCATGTTTGCAAAGAAAGC GGGACTATCTGTTATAATGGAGACTGTCCGGACCTGGACAGGGTGTGCGAGTCAATATATGGAGCAGGTT CAGTGAATGCTCCATTTGCCTGCTATGAAGAAATCCAGGGCCAAAATGATAGGTTTGGCAACTGTGGCAA AGACAACCGAAACAGATATGTGTTCTGTGGATGGAGGAATCTCATATGTGGGAGATTGATTTGTACGTAC CCTACCCGAATGCCTTACAACCCACCCAATAACAGTACAGCTTCTGTGATCTATGCTTTTGTGCGAGACA AAGTGTGTATAACTGTGGACTTTGGATCGAGTGTCAAAGAAGATCCACTCAGGGTTGCTAATGGTGCTAC ATGTGATCTTGATAGGATCTGCTTAAATGGCGTGTGCGTAGAATCGAGATTTCTTAGGGATCAATCAAAA ACGTGTTCATCCAAGTGCCACGGGAACGGAGTGTGCAATTCTCATGGTGTCTGCCACTGCAATGCCGGCT ACTCACCTCCCAACTGTCAATATCCTACGACAAAAAGGTCAGCATCGCTGTGGTCTGGCAAGCACGATCT GCCCATGGAAAGAGCATCTAAGAACCAAGAAAAGAAGTGGCTTTTAAGTTTGTACATTGTCTTAATTATC CTCGCTTCAGTGTTCCTCATAGGCACAGGGTGGAAAGGCTTGAAACAGTGCGGTTCCAAGGAAGAGGAGT CCATGAGTAGCGAAAGCAAATCAGAAGACAGCACTTACACGTATGTCAGCAGATCAACTTCTGAGACAAG CAGTATGACATCTACGAGCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210459 representing NM_153397
Red=Cloning site Green=Tags(s) MLGAMLHTLLLLLLAELGALLASGPESQSSFLEIIFPEKIEDKTHSEEQISYIIPINKKQYTVHLQKRYF LTNRFMVYMYNQGSTSFHSPNIPAQCYYQGHIKGYPNSVATLSTCSGLRGFLQFENVSYGIEPLQSAFTS QHIVYKLGNKEKELIFNKNSRNIEMPTNYGILINKKPKSPFKNLFPLYLEMSIVVDKALYDYLGSDSNIV TNKIIEIISLINSVFAQLKVTIVLSSLELWSDKNKIPTVGEADELLHKFLEWKQAYLTLRPHDVAYLFIY NEYPNYMGATYPGKMCTAHYSAGITMYPKDMTLEAFSVILTQMLGLSLGISYDEPEKCYCSESICIMNPR AMQYGGVKSFSNCSLNDFEHFKSNEGAKCLQNKPQMQRTAAAVCGNGKVEGDEICDCGSEAECGPDSCCE PNRCVLKAGRACDSKSPSSTCCKNCQFLPEKHQCRPEKHLYCDIPEVCNGSSGNCPPDVTINNGHVCKES GTICYNGDCPDLDRVCESIYGAGSVNAPFACYEEIQGQNDRFGNCGKDNRNRYVFCGWRNLICGRLICTY PTRMPYNPPNNSTASVIYAFVRDKVCITVDFGSSVKEDPLRVANGATCDLDRICLNGVCVESRFLRDQSK TCSSKCHGNGVCNSHGVCHCNAGYSPPNCQYPTTKRSASLWSGKHDLPMERASKNQEKKWLLSLYIVLII LASVFLIGTGWKGLKQCGSKEEESMSSESKSEDSTYTYVSRSTSETSSMTSTSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153397 |
| ORF Size | 2262 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153397.2, NP_700446.2 |
| RefSeq Size | 2447 bp |
| RefSeq ORF | 2265 bp |
| Locus ID | 353188 |
| UniProt ID | Q8K410 |
| MW | 84.4 kDa |
| Gene Summary | This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene was found to be expressed predominantly in the pachytene spermatocytes, where the processed protein is localized to the sperm surface. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221409 | Adam32 (untagged) - Mouse a disintegrin and metallopeptidase domain 32 (Adam32), (10ug) |
CNY 9,790.00 |
|
| MG210459 | Adam32 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 32 (Adam32) |
CNY 7,320.00 |
|
| MR210459L3 | Lenti ORF clone of Adam32 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 32 (Adam32) |
CNY 8,550.00 |
|
| MR210459L4 | Lenti ORF clone of Adam32 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 32 (Adam32) |
CNY 8,550.00 |
