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Mthfd1l (BC030437) Mouse Tagged ORF Clone
CAT＃: MR210353
- TrueORF®
Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413)
ORF Plasmid: tGFP
"BC030437" in other vectors (4)
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CNY 5,368.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410004L15Rik; AI647056; Fthfsdc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210353 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCCAGACAAGCTTCGAGAGGCAGACATTGTGGTCT TAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGCGTGGATTCCATCAGGAACCACCATTCTCAATTGCTT CCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTCCTGGGGTCCCCGTGGACAAACTCATTGCAGAA GAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCAGAACATGGTGAGCAGCGGCAGGAGGTGGCTCA GAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTGAAACTGCAGCCTCTCTCACCTGTGCCCAGTGA TATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGGATGTCCTTGCCAAGGAGATAGGATTGCTTGCA GATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGTCCAATTGTCCCTGCTGGAGAGGTTAAAGGATC AAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACGCCCACCCCGCTCGGAGAAGGGAAGAGTACAGT CACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGAAAGTCAACTCCTTCGCCTGTCTGAGGCAGCCT TCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGCAGGAGGTGGATATGCTCAGGTCATCCCCATGG AGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCCATCACTGCCGCTAATAACTTGCTGGCCGCAGC CATCGACACGAGAATTTTACATGAGAGCACTCAAACAGACAAGGCTCTCTACAATCGACTGGTTCCCTTA GTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTCTCGGCTGAAGAAACTGGGGATACATAAGACTG ATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTCGCCCGCCTCAACATTGACCCCGCAACCATCAC GTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGAGAAAAATAACCATTGGACAGGGAAGTACAGAG AAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGTGGCCAGCGAGATCATGGCCGTGCTGGCCTTGA CTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGAATGGTGGTGGCCAGTGACAAAGACGGGCAGCC TGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGACAGTTTTGATGAAAGACGCCATCAAGCCAAAT CTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCATGCTGGTCCCTTTGCCAACATCGCTCACGGCA ACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTGGTCGGCGAAGAAGGGTTTGTGGTGACTGAAGC TGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCAATATCAAATGCCGAGCTTCTGGCCTGGTGCCC AACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAAGATGCATGGAGGCGGGCCGAGTGTAACTGCTG GGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATTCAGCTGGTAGCTGATGGCTGCTGCAACCTACA GAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGGTGGTGGTGGCACTGAATGTCTTCAAGACGGAC ACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAAACGGGCCGGTGCCTTTGATGCGGTCCCCTGCT ATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTGGCCCGGGCTGTGAGAGAAGCTGCGAATAAAAG AAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCATCGTGGAAAAGATAAGAGTCATTGCCCAGACT GTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGCGCAGTCCAAAATCGATCGCTATACGCAACAGG GTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCACCTCTCTCTCTCTCATGAACCTGACAAAAAGGG AGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCCGGGCCAGCATCGGGGCTGGGTTCATTTATCCT TTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCACCCGACCCTGCTTTTATGATATAGACCTTGACA CAGAAACGGAGCAAGTTAAAGGCTTGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210353 protein sequence
Red=Cloning site Green=Tags(s) MTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAAWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAE ESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLA DEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQP SQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPL VNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTE KGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPN LMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVP NVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTD TRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQT VYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYP LVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC030437 |
| ORF Size | 2199 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC030437, AAH30437 |
| RefSeq Size | 3061 bp |
| RefSeq ORF | 2201 bp |
| Locus ID | 270685 |
| MW | 79.5 kDa |
| Gene Summary | May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220946 | Mthfd1l (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413),, (10ug) |
CNY 9,120.00 |
|
| MG210353 | Mthfd1l (tGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) |
CNY 4,560.00 |
|
| MR210353L3 | Lenti ORF clone of Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) |
CNY 6,080.00 |
|
| MR210353L4 | Lenti ORF clone of Mthfd1l (mGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) |
CNY 6,080.00 |
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