Rps6ka2 (NM_011299) Mouse Tagged ORF Clone
CAT#: MR210349
- TrueORF®
Rps6ka2 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 2 (Rps6ka2)
ORF Plasmid: tGFP
"NM_011299" in other vectors (4)
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CNY 5,376.00
CNY 6,460.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 90kDa; D17Wsu134e; p90rsk; pp90rsk; Rps6ka-rs1; Rsk3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210349 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGAGCATGAAGAAGTTCACGGTGCGCAGGTTCTTCTCCGTGTACCTGCGCAAGAAGTCGCGCT CCAAGAGCTCCAGTCTGAGTCGCCTCGAGGAAGAAGGCATTGTGAAGGAGATTGACATTAGCAACCATGT GAAGGAAGGCTTTGAGAAGGCAGACCCCTCCCAGTTCGAGCTACTAAAGGTTTTAGGACAAGGGTCGTAT GGAAAGGTGTTCTTGGTGAGGAAGGTTACAGGATCAGACGCTGGTCAGCTCTACACCATGAAGGTCCTGA AGAAAGCCACCTTGAAAGTGCGAGACCGGGTCAGATCTAAGATGGAGAGAGACATCCTGGCAGAGGTGAA TCACCCTTTCATTGTCAAGCTGCATTATGCCTTTCAGACCGAAGGCAAGCTCTACCTGATCCTGGACTTC CTGCGGGGAGGTGACCTCTTCACCAGGCTTTCCAAAGAGGTGATGTTCACGGAGGAGGATGTCAAGTTCT ACCTGGCTGAGCTGGCCTTGGCTCTAGACCACCTCCATGGCCTGGGGATCATCTACAGGGATCTGAAGCC AGAGAATATCCTCCTGGATGAAGAGGGACACATTAAGATCACAGATTTTGGCTTGAGCAAGGAGGCCACC GACCATGACAAGAGAGCCTATTCATTCTGTGGGACTATTGAATACATGGCGCCCGAGGTGGTGAACCGGC GTGGACACACACAGAGTGCCGACTGGTGGTCCTTCGGTGTGCTCATGTTCGAGATGCTCACAGGGTCCCT GCCATTCCAGGGGAAGGACAGGAAGGAAACAATGGCCCTCATCCTCAAAGCCAAGCTGGGTATGCCTCAG TTCCTCAGTGCGGAGGCTCAGAGCCTGCTCAGGGCCCTTTTCAAGCGGAACCCCTGCAACAGGCTAGGTG CTGGTGTTGATGGAGTGGAGGAAATTAAACGTCACCCGTTCTTTGTCACCATAGACTGGAATAAGCTGTA CCGCAAGGAGATCAAGCCACCCTTCAAGCCAGCAGTGGGCAGGCCTGAGGACACCTTCCACTTTGACCCC GAGTTTACTGCAAGGACCCCCACAGATTCTCCTGGTGTCCCCCCAAGTGCAAACGCCCACCACCTCTTCA GAGGATTCAGCTTTGTGGCCTCCAGCCTGGTCCAGGAGCCCTCACAGCAAGACGTGCCCAAGGCCCCCAT TCACCCAATTGTGCAGCAGCTACATGGGAACAACATCCACTTCACTGACGGCTATGAGATCAAGGAGGAC ATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGGTGTGTACACAAAGCCACGGATGCTGAGTATGCTGTGA AGATCATCGATAAGAGCAAAAGGGACCCCTCGGAGGAGATTGAGATCCTCCTGCGCTATGGCCAGCACCC CAACATCATCACCCTAAAAGATGTCTATGATGATGGCAAGTACGTCTACCTGGTGATGGAGCTCATGCGA GGCGGGGAGCTGCTGGACCGTATCCTCCGTCAGCGGTGCTTCTCAGAGCGTGAGGCCAGTGATGTGCTCT ATACCATCGCCAGGACCATGGACTACCTGCACTCCCAAGGGGTTGTCCATCGGGACCTGAAACCAAGTAA CATTCTGTACATGGATGAGTCTGGAAACCCCGAATCTATCCGCATCTGTGACTTTGGGTTTGCCAAACAG CTTCGAGCGGAGAATGGGCTGCTCATGACCCCCTGCTATACTGCAAACTTTGTAGCTCCCGAGGTCTTGA AGCGGCAAGGCTACGATGCAGCGTGTGATGTCTGGAGCTTGGGAATCCTGCTGTACACCATGCTGGCTGG GTTCACTCCGTTTGCCAATGGCCCAGACGATACCCCTGAGGAGATTCTGGCTAGGATCGGCAGTGGGAAG TACGCCCTTTCTGGGGGAAACTGGGACTCCATATCTGATGCAGCAAAAGATGTCGTGTCCAAGATGCTCC ACGTGGACCCTCAGCAACGCCTAACAGCAGTTCAAGTGTTGAAGCACCCGTGGATCGTGAACAGAGAGTA CCTATCCCAAAACCAGCTGAGCAGACAGGATGTCCATCTAGTGAAGGGTGCCATGGCGGCCACCTACTTT GCTCTGAACAGGACCCCACAGGCACCGAGGCTAGAGCCTGTGCTCTCATCTAGCTTGGCCCAACGCAGAG GCATGAAGAGACTCACGTCTACCAGGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210349 protein sequence
Red=Cloning site Green=Tags(s) MELSMKKFTVRRFFSVYLRKKSRSKSSSLSRLEEEGIVKEIDISNHVKEGFEKADPSQFELLKVLGQGSY GKVFLVRKVTGSDAGQLYTMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDF LRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHGLGIIYRDLKPENILLDEEGHIKITDFGLSKEAT DHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQ FLSAEAQSLLRALFKRNPCNRLGAGVDGVEEIKRHPFFVTIDWNKLYRKEIKPPFKPAVGRPEDTFHFDP EFTARTPTDSPGVPPSANAHHLFRGFSFVASSLVQEPSQQDVPKAPIHPIVQQLHGNNIHFTDGYEIKED IGVGSYSVCKRCVHKATDAEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKYVYLVMELMR GGELLDRILRQRCFSEREASDVLYTIARTMDYLHSQGVVHRDLKPSNILYMDESGNPESIRICDFGFAKQ LRAENGLLMTPCYTANFVAPEVLKRQGYDAACDVWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGK YALSGGNWDSISDAAKDVVSKMLHVDPQQRLTAVQVLKHPWIVNREYLSQNQLSRQDVHLVKGAMAATYF ALNRTPQAPRLEPVLSSSLAQRRGMKRLTSTRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011299 |
ORF Size | 2202 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_011299.1, NM_011299.2, NM_011299.3, NM_011299.4, NP_035429.1 |
RefSeq Size | 5435 bp |
RefSeq ORF | 2202 bp |
Locus ID | 20112 |
UniProt ID | Q9WUT3 |
MW | 83.2 kDa |
Gene Summary | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202598 | Rps6ka2 (untagged) - Mouse ribosomal protein S6 kinase, polypeptide 2 (Rps6ka2), (10ug) |
CNY 5,376.00 |
|
MG210349 | Rps6ka2 (tGFP-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 2 (Rps6ka2) |
CNY 7,130.00 |
|
MR210349L3 | Lenti ORF clone of Rps6ka2 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 2 (Rps6ka2) |
CNY 8,360.00 |
|
MR210349L4 | Lenti ORF clone of Rps6ka2 (mGFP-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 2 (Rps6ka2) |
CNY 8,360.00 |
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