Rps6ka2 (NM_011299) Mouse Tagged ORF Clone

CAT#: MR210349

  • TrueORF®

Rps6ka2 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 2 (Rps6ka2)

ORF Plasmid: DDK tGFP



  "NM_011299" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,376.00

CNY 6,460.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rps6ka2 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 90kDa; D17Wsu134e; p90rsk; pp90rsk; Rps6ka-rs1; Rsk3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210349 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGAGCATGAAGAAGTTCACGGTGCGCAGGTTCTTCTCCGTGTACCTGCGCAAGAAGTCGCGCT
CCAAGAGCTCCAGTCTGAGTCGCCTCGAGGAAGAAGGCATTGTGAAGGAGATTGACATTAGCAACCATGT
GAAGGAAGGCTTTGAGAAGGCAGACCCCTCCCAGTTCGAGCTACTAAAGGTTTTAGGACAAGGGTCGTAT
GGAAAGGTGTTCTTGGTGAGGAAGGTTACAGGATCAGACGCTGGTCAGCTCTACACCATGAAGGTCCTGA
AGAAAGCCACCTTGAAAGTGCGAGACCGGGTCAGATCTAAGATGGAGAGAGACATCCTGGCAGAGGTGAA
TCACCCTTTCATTGTCAAGCTGCATTATGCCTTTCAGACCGAAGGCAAGCTCTACCTGATCCTGGACTTC
CTGCGGGGAGGTGACCTCTTCACCAGGCTTTCCAAAGAGGTGATGTTCACGGAGGAGGATGTCAAGTTCT
ACCTGGCTGAGCTGGCCTTGGCTCTAGACCACCTCCATGGCCTGGGGATCATCTACAGGGATCTGAAGCC
AGAGAATATCCTCCTGGATGAAGAGGGACACATTAAGATCACAGATTTTGGCTTGAGCAAGGAGGCCACC
GACCATGACAAGAGAGCCTATTCATTCTGTGGGACTATTGAATACATGGCGCCCGAGGTGGTGAACCGGC
GTGGACACACACAGAGTGCCGACTGGTGGTCCTTCGGTGTGCTCATGTTCGAGATGCTCACAGGGTCCCT
GCCATTCCAGGGGAAGGACAGGAAGGAAACAATGGCCCTCATCCTCAAAGCCAAGCTGGGTATGCCTCAG
TTCCTCAGTGCGGAGGCTCAGAGCCTGCTCAGGGCCCTTTTCAAGCGGAACCCCTGCAACAGGCTAGGTG
CTGGTGTTGATGGAGTGGAGGAAATTAAACGTCACCCGTTCTTTGTCACCATAGACTGGAATAAGCTGTA
CCGCAAGGAGATCAAGCCACCCTTCAAGCCAGCAGTGGGCAGGCCTGAGGACACCTTCCACTTTGACCCC
GAGTTTACTGCAAGGACCCCCACAGATTCTCCTGGTGTCCCCCCAAGTGCAAACGCCCACCACCTCTTCA
GAGGATTCAGCTTTGTGGCCTCCAGCCTGGTCCAGGAGCCCTCACAGCAAGACGTGCCCAAGGCCCCCAT
TCACCCAATTGTGCAGCAGCTACATGGGAACAACATCCACTTCACTGACGGCTATGAGATCAAGGAGGAC
ATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGGTGTGTACACAAAGCCACGGATGCTGAGTATGCTGTGA
AGATCATCGATAAGAGCAAAAGGGACCCCTCGGAGGAGATTGAGATCCTCCTGCGCTATGGCCAGCACCC
CAACATCATCACCCTAAAAGATGTCTATGATGATGGCAAGTACGTCTACCTGGTGATGGAGCTCATGCGA
GGCGGGGAGCTGCTGGACCGTATCCTCCGTCAGCGGTGCTTCTCAGAGCGTGAGGCCAGTGATGTGCTCT
ATACCATCGCCAGGACCATGGACTACCTGCACTCCCAAGGGGTTGTCCATCGGGACCTGAAACCAAGTAA
CATTCTGTACATGGATGAGTCTGGAAACCCCGAATCTATCCGCATCTGTGACTTTGGGTTTGCCAAACAG
CTTCGAGCGGAGAATGGGCTGCTCATGACCCCCTGCTATACTGCAAACTTTGTAGCTCCCGAGGTCTTGA
AGCGGCAAGGCTACGATGCAGCGTGTGATGTCTGGAGCTTGGGAATCCTGCTGTACACCATGCTGGCTGG
GTTCACTCCGTTTGCCAATGGCCCAGACGATACCCCTGAGGAGATTCTGGCTAGGATCGGCAGTGGGAAG
TACGCCCTTTCTGGGGGAAACTGGGACTCCATATCTGATGCAGCAAAAGATGTCGTGTCCAAGATGCTCC
ACGTGGACCCTCAGCAACGCCTAACAGCAGTTCAAGTGTTGAAGCACCCGTGGATCGTGAACAGAGAGTA
CCTATCCCAAAACCAGCTGAGCAGACAGGATGTCCATCTAGTGAAGGGTGCCATGGCGGCCACCTACTTT
GCTCTGAACAGGACCCCACAGGCACCGAGGCTAGAGCCTGTGCTCTCATCTAGCTTGGCCCAACGCAGAG
GCATGAAGAGACTCACGTCTACCAGGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210349 protein sequence
Red=Cloning site Green=Tags(s)

MELSMKKFTVRRFFSVYLRKKSRSKSSSLSRLEEEGIVKEIDISNHVKEGFEKADPSQFELLKVLGQGSY
GKVFLVRKVTGSDAGQLYTMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDF
LRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHGLGIIYRDLKPENILLDEEGHIKITDFGLSKEAT
DHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQ
FLSAEAQSLLRALFKRNPCNRLGAGVDGVEEIKRHPFFVTIDWNKLYRKEIKPPFKPAVGRPEDTFHFDP
EFTARTPTDSPGVPPSANAHHLFRGFSFVASSLVQEPSQQDVPKAPIHPIVQQLHGNNIHFTDGYEIKED
IGVGSYSVCKRCVHKATDAEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKYVYLVMELMR
GGELLDRILRQRCFSEREASDVLYTIARTMDYLHSQGVVHRDLKPSNILYMDESGNPESIRICDFGFAKQ
LRAENGLLMTPCYTANFVAPEVLKRQGYDAACDVWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGK
YALSGGNWDSISDAAKDVVSKMLHVDPQQRLTAVQVLKHPWIVNREYLSQNQLSRQDVHLVKGAMAATYF
ALNRTPQAPRLEPVLSSSLAQRRGMKRLTSTRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011299
ORF Size 2202 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011299.1, NM_011299.2, NM_011299.3, NM_011299.4, NP_035429.1
RefSeq Size 5435 bp
RefSeq ORF 2202 bp
Locus ID 20112
UniProt ID Q9WUT3
MW 83.2 kDa
Gene Summary Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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