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Afap1 (NM_027373) Mouse Tagged ORF Clone
CAT＃: MR210338
- TrueORF®
Afap1 (Myc-DDK-tagged) - Mouse actin filament associated protein 1 (Afap1)
ORF Plasmid: tGFP
"NM_027373" in other vectors (4)
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CNY 5,352.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600003E23Rik; 9630044L16Rik; Afap; AI848729; mKIAA3018 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210338 representing NM_027373
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGTTAATAGTTGAACTCCGCCTCTTTCTGGAGCTCCTGGACCACGAGTACCTCACATCAACTG TCAGAGAGAAAAAGGCTGTGCTCACCAACATCCTGCTGCGCCTGCAGTCATCCAAGGGCTTTGAAGTGAA GGACCATGCTCAGAAGGCAGAGGCCAACAACCTGCCGGCACCACCCCAGATGCCTCTGCCGGAGATCCCT CAGCCATGGCTGCCTCCTGACAGCGGGCCTCCGCCTTTACCAACGTCCTCTCTCCCAGAGGGTTACTATG AGGAAGCCGTGCCGCTGAGCCCTGGGAAAGCTCCAGAGTACATCACCTCAAATTATGACTCTGATGCCAT GAGCAGTTCCTATGAGTCATATGATGAAGAGGAAGAGGACGGGAAGGGGAAGAAAACTCGGCATCAGTGG CCGTCAGAGGAGGCCTCTATGGACCTGGTGAAAGACGCCAAGATCTGTGCCTTCCTGCTGCGCAAGAAAC GCTTTGGCCAGTGGACCAAGCTGCTCTGCGTCATCAAAGACACCAAGCTGCTGTGCTATAAAAGTTCCAA GGACCAGCAGCCTCAGATGGAGCTGCCGCTGCAGGGCTGCAGTATCACATACATCCCGAGAGACAGCAAG AAGAAGAAACACGAGCTGAAAATCACCCAGCAAGGCACAGACCCTCTGGTTCTAGCTGTTCAGAGCAAGG AACAGGCTGAGCAGTGGTTGAAGGTAATCAAAGAAGCCTACAGTGGCTGTAGCGGGCCCGTGGATCCGGA ATGTTCTCCACCACCCAGCACCAGCGCCCCCGTGAACAAGGCAGAACTGGAGAAGAAATTGTCTTCAGAG AGGCCCAGCTCTGATGGAGAAGGTGGTGTGGAAAACGGAGTCACCACGTGTAATGGAAAAGAACAAGCAA AGAGGAAGAAACCTTCCAAGTCAGAGGCCAAGGGGACTGTGTCCAAGGTCACTGGGAAGAAAATTACCAA GATCATCGGTCTGGGAAAGAAGAAGCCATCCACAGATGAGCAGACATCATCAGCAGAGGAGGATGTGCCC ACTTGTGGCTACCTGAATGTGCTCTCTAACAGCCGCTGGCGGGAGCGCTGGTGCAGAGTGAAGGACAGCA AACTCATTCTCCACAAGGACAGGGCTGACCTGAAGACCCACCTCGTCTCCATCCCCCTCCGTGGCTGCGA GGTGATCCCGGGCCTGGATTCTAAGCACCCGTTGACCTTCCGGCTGCTTCGAAACGGACAGGAGGTGGCT GTTCTAGAGGCATCATCGTCTGAAGACATGGGCAGGTGGATTGGCATCTTGCTGGCTGAGACGGGGTCCT CCACGGACCCAGGAGCGCTGCACTACGACTACATCGATGTGGAGATGTCAGCAAATGTCATCCAGACGGC GAAACAGACCTTCTGCTTTATGAACAGGCGGGCAGTGTCCACTAGCCCATACCTGGGGAGCCTCTCCAAC GGCTACGCCCATCCCAGTGGGACAGCACTACACTATGATGACGTCCCCTGTGTCAACGGCTCGTTGAAGA ACAAAAAACCGCCAGCGTCATCTAATGGGGTTCCTGTAAAGGGGAAGGCTCCCAGCAGTCAGCAAAAAAA GGTGGAAACTGCAGGTGGTGTGAAACGGACAGCATCAAATGCTGAGCAGTACAAATATGGGAAGAACCGG GTAGAAGCAGATGCCAAGCGGTTACAGTCCAAAGAGGAAGAGCTGCTGAAGAGGAAAGAGGCCCTACGGA ACAGGCTGGCACAGCTGCGGAAAGAGAGGAAGGACCTGAGGGCCGCCATCGAAGTGAATGCAGGCAGGAA GACCCAGGCAGCTCTGGAGGACAAGTTGAAGAGGCTGGAGGAAGAGTGCAAGCAGAGGGAGGCGGAGCGT GTCAGCCTGGAGCTGGAGCTGACCGAGGTCAAGGAGAGCCTGAAGAAGGCCTTGGCGGGTGGCGTCACCC TAGGACTGGCCATCGAGCCCAGGTCAGGGACATCCAGTCCACAGTCTCCTGTGTTTCGGCATCGGACTCT GGAAAATTCTCCCATCTCCAGCTGTGATACAAGCGATGCTGAGGGGCCTTTGCCTGTGAACAGTGCAGCC GTCCTGAAGAAGAGCCAGCCGTCCTCAGGCAGCTCCCCTTGCCGTGGCCACGTGCTGCAGAAGGCCAAGG AGTGGGAGCTGAAGAATGGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210338 representing NM_027373
Red=Cloning site Green=Tags(s) MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRLQSSKGFEVKDHAQKAEANNLPAPPQMPLPEIP QPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQW PSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSK KKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSE RPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVP TCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVA VLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSN GYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNR VEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAER VSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAA VLKKSQPSSGSSPCRGHVLQKAKEWELKNGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027373 |
| ORF Size | 2193 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027373.2, NP_081649.1 |
| RefSeq Size | 6617 bp |
| RefSeq ORF | 2196 bp |
| Locus ID | 70292 |
| UniProt ID | Q80YS6 |
| MW | 81.1 kDa |
| Gene Summary | Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204907 | Afap1 (untagged) - Mouse actin filament associated protein 1 (Afap1), (10ug) |
CNY 5,360.00 |
|
| MG210338 | Afap1 (tGFP-tagged) - Mouse actin filament associated protein 1 (Afap1) |
CNY 6,370.00 |
|
| MR210338L3 | Lenti ORF clone of Afap1 (Myc-DDK-tagged) - Mouse actin filament associated protein 1 (Afap1) |
CNY 7,700.00 |
|
| MR210338L4 | Lenti ORF clone of Afap1 (mGFP-tagged) - Mouse actin filament associated protein 1 (Afap1) |
CNY 7,700.00 |
