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Rbak (BC059913) Mouse Tagged ORF Clone
CAT＃: MR210196
- TrueORF®
Rbak (Myc-DDK-tagged) - Mouse RB-associated KRAB repressor (cDNA clone MGC:69906 IMAGE:6825552)
ORF Plasmid: tGFP
"BC059913" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210196 representing BC059913
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTGCAGGGCCCACTGTCATTCAAGGATGTGGCTGTGGCCTTCTCCCAGGAGGAGTGGCAGCAGC TGGACCCTGAGGAGAGGACCACATACAGGGATGTGATGCTGGAGACCTACAGCAACCTCGTCTCCGTGGG GTATGACGTTACCAAGCCAAACATGATTATTAAGTTGGAGCAGGGAGAAGAGCCCTGGACAGTGGAAGGT GACCGCCATGCTCAGAGGCATTTGGAAATCAGTAAAGTTTATGACCCCAGAGAAGGAATTGAGGAAATTG GCGAGAAACATCTGCAGTGCGATGATGATCCCTACTGCTGGAGAGCTGAGAAGGGGGCTGCTTTTGATGA AGCCTACACCCTCGAAACAGCCCTCATTTCCCCCAGCTCAGGTGCTCACAGCTGTGTCTCCTGTGGGGAG ACCCTGGAGTCTGTTTCAGAACTGATTAGCAGTGACGGAAGCTATGCACTGGAGAAGCCCAGCATGTGTT TTGAGTGTGGGAAAGCATACGGAGAAAGCCTTGAAGACTTCAACCAAGATGAGGGTAACTCCTCTCAACA CGATGAAAATATTTTGCAGAAAGTCACCATTTTGGAGAAACCCTTTGCTTATGAGTGCATGGAAGCCTTA GACAGTGAAAGTGTTTTCATGGCTCGTGAGAGAGCTTACATGGGGGAGAAGCCCTATGACTGGGGAGACT CTGGACCAGACTTCATCCAGATGTCGGACTTCAGTACATATCCGAGATCACAAATGGAACTGAAGCCCTT CGAATGTACACAGTGTGGGAAGTCCTTCTGTAAGAAGTCCAAATTCATCATCCACCAGAGAGCTCACACA GGAGAGAAGCCGTACGCTTGTAGTGTGTGTGGAAAGTCCTTCAGTCAAAAGGGAACCCTCACTGTACATC GGAGATCGCACTTAGAGGAGAAGCCCTATAAGTGTAATGAGTGTGGGAAGACCTTTTGTCAGAAGTTACA CCTCACACAGCACCAGAGGACTCACTCAGGAGAGAAGCCCTATGAATGTAGCGAGTGCGGGAAGTCCTTC TGCCAGAAGACACACCTCACTCTCCACCAGAGGAATCACTCAGGCGAGAGGCCCTACCCGTGTAATGAAT GTGGGAAATCCTTCTCCCGGAAGTCTGCCCTCAATGACCATCAGAGGACACACACAGGAGAGAAGCTCTA TAAGTGTAATGAGTGTGGGAAATCCTACTACCGGAAGTCCACCCTCATTACACACCAGAGGACACACACA GGGGAGAAGCCCTATCAGTGTAGTGAGTGTGGGAAATTCTTCTCTCGGGTGTCGTACCTCACCATACACT ATCGGAGCCATTTAGAAGAGAAACCTTACGAGTGTACAGAATGTGGGAAAACCTTCAATCTAAACTCTGC TTTCATTAGGCACTGGAAGGTCCATGCAGAAGAGAGAGTCCAGGAGTGTGGCGAGTGTGGGAAGCCATCC CCGCTGCAGTGTGCCCCTGATCACACAGGCGACCTAGGAGAGAAGCGCTATGAGTGTAATGAGTGTGGAA AGACCTTCCTTGACAGCTCGGCCTTCCATAGGCACCAGTCCGTCCCTGAAGGGGAGAAAACTTACGAGTG TAATATATGCGGAAAGTCCTTCTCGGATTCGTCATGCTACACTGTGCACTACAGAGGTCATTCCGAAGAG AAGCCCTTCGGGTGCAGTGAATGTGGGAAGACCTTCTCTCATAACTCATCCCTCTTCAGACATCAAAGGG TCCACACCGGCGAGAAGCCGTACGAGTGTTACGAATGTGGGAAGTTCTTCTCTCAGAAGTCGTATCTCAC TATCCATCACCGGATCCATTCGGGAGAGAAGCCCTACGAGTGCAGCAAGTGTGGGAAAGTGTTCTCGCGG ATGTCGAACCTCACCGTACACTACCGAAGCCATTCAGGAGAGAAGCCGTATGAGTGCAACGAGTGTGGGA AAGTCTTTTCTCAGAAGTCGTACCTCACCGTGCATTACCGGACTCATTCAGGAGAGAAACCTTATGAATG TAACGAATGTGGGAAAAAATTCCACCACAGATCAGCCTTCAATAGCCATCAGAGAATTCATAAAAGAGGC ACTGTAAATGTACTCACTGTAGAAAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210196 representing BC059913
Red=Cloning site Green=Tags(s) MSLQGPLSFKDVAVAFSQEEWQQLDPEERTTYRDVMLETYSNLVSVGYDVTKPNMIIKLEQGEEPWTVEG DRHAQRHLEISKVYDPREGIEEIGEKHLQCDDDPYCWRAEKGAAFDEAYTLETALISPSSGAHSCVSCGE TLESVSELISSDGSYALEKPSMCFECGKAYGESLEDFNQDEGNSSQHDENILQKVTILEKPFAYECMEAL DSESVFMARERAYMGEKPYDWGDSGPDFIQMSDFSTYPRSQMELKPFECTQCGKSFCKKSKFIIHQRAHT GEKPYACSVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHQRTHSGEKPYECSECGKSF CQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALNDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTHT GEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECTECGKTFNLNSAFIRHWKVHAEERVQECGECGKPS PLQCAPDHTGDLGEKRYECNECGKTFLDSSAFHRHQSVPEGEKTYECNICGKSFSDSSCYTVHYRGHSEE KPFGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFSR MSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHKRG TVNVLTVEKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059913 |
| ORF Size | 2130 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059913 |
| RefSeq Size | 3256 bp |
| RefSeq ORF | 2132 bp |
| Locus ID | 57782 |
| MW | 119.4 kDa |
| Gene Summary | May repress E2F-dependent transcription. May promote AR-dependent transcription.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220804 | Rbak (untagged) - Mouse RB-associated KRAB repressor (cDNA clone MGC:69906 IMAGE:6825552), (10ug) |
CNY 9,120.00 |
|
| MG210196 | Rbak (tGFP-tagged) - Mouse RB-associated KRAB repressor (cDNA clone MGC:69906 IMAGE:6825552) |
CNY 7,890.00 |
|
| MR210196L3 | Lenti ORF clone of Rbak (Myc-DDK-tagged) - Mouse RB-associated KRAB repressor (cDNA clone MGC:69906 IMAGE:6825552) |
CNY 9,030.00 |
|
| MR210196L4 | Lenti ORF clone of Rbak (mGFP-tagged) - Mouse RB-associated KRAB repressor (cDNA clone MGC:69906 IMAGE:6825552) |
CNY 9,030.00 |
