C1r (BC004637) Mouse Tagged ORF Clone

CAT#: MR210176

  • TrueORF®

C1r (Myc-DDK-tagged) - Mouse complement component 1, r subcomponent (cDNA clone MGC:6404 IMAGE:3586055)

ORF Plasmid: DDK tGFP



  "BC004637" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,176.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms C1rb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210176 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCTCTTTGCCCTCCTGGTGACCCTGTTCTATGGGGTGGAAGGCTCCATTTACCTCCCTCAGAAGC
TCTATGGAGAGGTGACCTCCCCTCTGTATCCCAAGCCTTACCCCAGTGACTTGGAGACAACCACTGTGAT
CACTGTCCCCATGGGGTACAGGGTGAAGCTGGTCTTCTGGCAGTTTGACGTGGAGCCTTCTGAAGGCTGC
TTCTATGACTATGTTAAGATTTCTGCTGATAAGCAAACACTGGGGAGGTTCTGTGGGCAGCTGGATTCTC
CCCTGGGCAACCCCCCAGGAAGCAAGGAATTCATGTCCCAAGGAAACAAGATGCTGCTGACCTTCCACAC
AGACTTCTCCAATGAGGAGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAGGCTGTA
GACCTTGATGAATGTGCATCGCAGCCCAACTCAGTGGAAGAGGGTTTGCAGCCCCGATGCCAACATCTGT
GTCACAACTATGTTGGAGGCTACTTCTGTTCCTGCCATCCTGGCTATGAGCTTCAGAAAGATGGGCAATC
CTGCCAGGCTGAGTGCAGCAGTGAGCTCTACACAGAGCCCTCAGGCTATGTCTCCAGCCTTGAATACCCT
CAGCCCTATCCACCGGATCTACGCTGCAACTACAGCATCCGGGTGGAGAGGGGCCTCACTGTGCACCTCA
AGTTCCTGGATCCCTTTGAAATTGATGACCACCAGCAAGTGCACTGCCCCTATGACCAGCTCCAGATCTA
CGCTAATGGGAAGAACTTGGGTGAATTCTGTGGAAAGCAAAGGCCTCCAGACCTTGACACCAGCAGCAAT
GCAGTGGATCTGCTGTTCTTCACAGATGAGTCAGGGGACAGCCGAGGCTGGAAGCTGCACTACACCACTG
AAACCATCAAGTGCCCCCAGCCCAAGGCTCTGGATGAGTTCACCATCATCCAGGATCCGCAGCCTCAGTA
CCAGTTCCGGGATTACTTCATTGTCACCTGCAAGCAAGGCTACCAGCTCATGGAGGGAAATCAGGCGCTA
CTCTCCTTCACAGCTGTTTGCCAGCATGATGGCACATGGCATCGTGCCATGCCCAGGTGCAAGATCAAGA
ACTGTGGGCAGCCCCAAAGCCTGTCTAATGGGGACTTCCGCTACATCACCACAAAAGGCGTGACCACCTA
TGAAGCCAGTATCCAGTATCACTGCCATGAACCATATTACAAGATGCTGACCAGAGCTGGCAGCAGCGAG
TCCATGCGAGGGATATATACCTGCACAGCCCAAGGCATTTGGAAGAATGAAGAGGAAGGAGAGAAAATGC
CCCGGTGTCTGCCAGTGTGTGGGAAACCTGTCAACCCTGTGACACAGAAGGAGCGCATCATCAGAGGGCA
GCCAGCCAGGCCCGGCAACTTCCCCTGGCAGGCCTTCACCACTACCCACGGGCGAGGGGGTGGGGCCCTG
CTTGGAGACCGCTGGATCCTCACAGCAGCCCACACCATCTACCCCAAGCATCACAACAAGGAAAACGACA
ATGCCAACCCCAAAATGCTTGTTTTCCTGGGCCACACAAATGTGGAACAGATCAAAAAACTGGGACATCA
CCCAGTCCGTAGGGTCATCATACACCCAGACTACCGCCAAGATGAACCTAACAATTTTGAAGGAGACATT
GCTCTACTGGAGCTGGAAAACAGTGTCACACTGGGCCCCGAACTCCTCCCCATCTGTCTCCCAGACAATG
AGACCTTCTATGGCCAAGGCCTCATGGGTTATGTCAGCGGATTCGGGATAACAGAAGATAAGTTAGCTTT
CGATCTCAGGTTCGTCAGACTGCCTGTAGCTGACAGTGAGGCATGCCAGAGATGGCTCCAGACAAAAAAG
GATACTTCTCCATTTTCTCAAAATATGTTCTGTTCTGGGGACCCAGCTGTACAGCAAGACGCCTGCCAAG
GGGACAGTGGGGGTGTTTTTGCAGTCAGGGACAGAAATCGTGATATCTGGGTGGCTACGGGCATCGTATC
CTGGGGCATTGGGTGTGGTGAGGGATATGGCTTCTACACCAAGGTACTGAATTATGTTGACTGGATCAAG
AAAGAGATGGGAGACGAAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210176 protein sequence
Red=Cloning site Green=Tags(s)

MWLFALLVTLFYGVEGSIYLPQKLYGEVTSPLYPKPYPSDLETTTVITVPMGYRVKLVFWQFDVEPSEGC
FYDYVKISADKQTLGRFCGQLDSPLGNPPGSKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQAV
DLDECASQPNSVEEGLQPRCQHLCHNYVGGYFCSCHPGYELQKDGQSCQAECSSELYTEPSGYVSSLEYP
QPYPPDLRCNYSIRVERGLTVHLKFLDPFEIDDHQQVHCPYDQLQIYANGKNLGEFCGKQRPPDLDTSSN
AVDLLFFTDESGDSRGWKLHYTTETIKCPQPKALDEFTIIQDPQPQYQFRDYFIVTCKQGYQLMEGNQAL
LSFTAVCQHDGTWHRAMPRCKIKNCGQPQSLSNGDFRYITTKGVTTYEASIQYHCHEPYYKMLTRAGSSE
SMRGIYTCTAQGIWKNEEEGEKMPRCLPVCGKPVNPVTQKERIIRGQPARPGNFPWQAFTTTHGRGGGAL
LGDRWILTAAHTIYPKHHNKENDNANPKMLVFLGHTNVEQIKKLGHHPVRRVIIHPDYRQDEPNNFEGDI
ALLELENSVTLGPELLPICLPDNETFYGQGLMGYVSGFGITEDKLAFDLRFVRLPVADSEACQRWLQTKK
DTSPFSQNMFCSGDPAVQQDACQGDSGGVFAVRDRNRDIWVATGIVSWGIGCGEGYGFYTKVLNYVDWIK
KEMGDEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC004637
ORF Size 2121 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC004637, AAH04637
RefSeq Size 2409 bp
RefSeq ORF 2123 bp
Locus ID 50909
MW 80.1 kDa
Gene Summary C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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