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Col8a2 (NM_199473) Mouse Tagged ORF Clone
CAT＃: MR210118
- TrueORF®
Col8a2 (Myc-DDK-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2)
ORF Plasmid: tGFP
"NM_199473" in other vectors (4)
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CNY 5,128.00
CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI429819 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210118 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGGGCTCTGATGCCCCTGCCTTCGCTGCTGCTGTTGCTGTTGGGGTGTGGGCCAAGAGTGTCCT CTGGCGGCGGAGCTGGGGGTGCAGCGGGCTATGCCCCGGTAAAGTATGTGCAGCCCATGCAGAAAGGACC CGTGGGACCGCCTTTCCGAGAGGGCAAAGGTCAGTACTTGGAAATGCCTCTACCGATGCTGCCAATGGAC TTGAAAGGAGAGCCAGGTCCCCCTGGGAAGCCGGGACCTCGGGGTCCCCCTGGGCCTCCTGGCTTCCCAG GAAAACCAGGCACTGGAAAACCTGGGGTTCATGGACAGCCAGGCCCTGCTGGCCCCCCTGGCTTCTCCCG AATGGGCAAAGCTGGTCCCCCAGGGCTCCCAGGCAAGGTTGGACCACCAGGGCAGCCAGGGCTACGGGGG GAGCCAGGGATACGAGGGGACCAGGGCCTCCGGGGGCCCCCAGGACCCCCTGGCCTTCCTGGTCCCTCAG GTATTACTGTCCCTGGAAAACCAGGTGCCCAGGGGGCTCCAGGGCCTCCAGGATTTAGGGGGGAGCCAGG ACCCCAGGGAGAGCCTGGGCCCCGAGGAGATAGGGGCCTCAAAGGGGATAATGGAGTGGGCCAACCAGGG CTGCCTGGAGCCCCTGGACAGGCAGGTGCCCCTGGGCCCCCTGGGCTGCCCGGTCCAGCTGGCTTGGGCA AACCAGGTTTGGATGGGATACCAGGAGCCCCAGGAGACAAAGGTGATTCTGGCCCCCCTGGGGTTCCAGG TTCTAGGGGAGAGCCAGGAGCTGTGGGTCCAAAAGGCCCTCCTGGGGTAGATGGTGTGGGGATACCAGGG GCAGCAGGGGTGCCAGGGCCACAGGGTCCAGTAGGGGCTAAAGGAGAGCCAGGGCTCCGGGGCCCCCCTG GCCTGATAGGCCCTGTTGGCTATGGGATGCCAGGAAAACCAGGACCTAAGGGAGACAGGGGCCCAGTTGG GGCCCCAGGGCTCTTAGGAGACAGAGGTGAGCCAGGAGAGGATGGGAAGCCAGGGGAGCAGGGTCCACAG GGACTTGGGGGTCCACCTGGCCTTCCTGGGTCTGCAGGGCTTCCTGGTAGACGTGGGCCCCCTGGGTCAA AGGGAGAGGTAGGACCTGGAGGTCCCCCAGGAGTGCCTGGCATTCGAGGAGACCAAGGGCCTAATGGCCT GGCTGGGAAGCCTGGGCTCCCTGGTGAGAGGGGACTTCCTGGGGCCCATGGACCCCCTGGACCGACTGGG CCTAAGGGTGAGCCAGGTTTTACAGGTCGCCCTGGAGGGCCAGGGGTGGCAGGAGCCCTAGGACAGAAAG GTGACTTGGGGCTTCCTGGGCAGCCTGGCTTGAGGGGTCCCTCTGGAATCCCAGGGCTCCAGGGCCCAGC TGGCCCTATTGGGCCTCAGGGTCTGCCAGGCCTGAAGGGTGAACCGGGCCTTCCAGGGCCTCCTGGAGAG GGAAAAGTAGGGGAGCCTGGCTCTGCTGGTCCCACAGGGCCCCCTGGAGTCCCTGGCTCCCCAGGACTCA CAGGTCCTCCTGGGCCCCCTGGGCCTCCTGGGCCTCCTGGTGCCCCCGGAGCCCTGGATGAGACTGGTAT CGCAGGCTTGCATCTGCCCAATGGTGGCGTGGAGGGAGCTGTACTGGGCAAAGGAGGCAAGCCACAGTTT GGGCTCGGTGAGCTGTCAGCCCACGCCACTCCAGCCTTTACTGCAGTGCTCACTTCCCCCTTCCCTGCCT CAGGCATGCCGGTTAGGTTTGACCGGACTCTCTACAATGGCCACAGCGGCTACAACCCAGCCACTGGCAT CTTCACCTGCCCCGTGGGAGGGGTCTACTACTTTGCATACCATGTGCATGTCAAGGGCACCAATGTGTGG GTAGCCCTGTACAAGAACAATGTGCCGGCCACCTACACGTACGACGAATACAAGAAGGGCTACCTGGACC AGGCTTCGGGAGGGGCTGTGCTCCAGCTGCGGCCCAATGACCAGGTGTGGGTGCAGATGCCCTCAGATCA GGCCAATGGTCTCTACTCCACCGAGTACATCCATTCGTCCTTCTCAGGATTCTTGCTCTGTCCCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210118 protein sequence
Red=Cloning site Green=Tags(s) MQGALMPLPSLLLLLLGCGPRVSSGGGAGGAAGYAPVKYVQPMQKGPVGPPFREGKGQYLEMPLPMLPMD LKGEPGPPGKPGPRGPPGPPGFPGKPGTGKPGVHGQPGPAGPPGFSRMGKAGPPGLPGKVGPPGQPGLRG EPGIRGDQGLRGPPGPPGLPGPSGITVPGKPGAQGAPGPPGFRGEPGPQGEPGPRGDRGLKGDNGVGQPG LPGAPGQAGAPGPPGLPGPAGLGKPGLDGIPGAPGDKGDSGPPGVPGSRGEPGAVGPKGPPGVDGVGIPG AAGVPGPQGPVGAKGEPGLRGPPGLIGPVGYGMPGKPGPKGDRGPVGAPGLLGDRGEPGEDGKPGEQGPQ GLGGPPGLPGSAGLPGRRGPPGSKGEVGPGGPPGVPGIRGDQGPNGLAGKPGLPGERGLPGAHGPPGPTG PKGEPGFTGRPGGPGVAGALGQKGDLGLPGQPGLRGPSGIPGLQGPAGPIGPQGLPGLKGEPGLPGPPGE GKVGEPGSAGPTGPPGVPGSPGLTGPPGPPGPPGPPGAPGALDETGIAGLHLPNGGVEGAVLGKGGKPQF GLGELSAHATPAFTAVLTSPFPASGMPVRFDRTLYNGHSGYNPATGIFTCPVGGVYYFAYHVHVKGTNVW VALYKNNVPATYTYDEYKKGYLDQASGGAVLQLRPNDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199473 |
| ORF Size | 2100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199473.1 |
| RefSeq Size | 4331 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 329941 |
| UniProt ID | P25318 |
| MW | 66.9 kDa |
| Gene Summary | Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220630 | Col8a2 (untagged) - Mouse collagen, type VIII, alpha 2 (Col8a2), (10ug) |
CNY 9,030.00 |
|
| MG210118 | Col8a2 (tGFP-tagged) - Mouse procollagen, type VIII, alpha 2 (Col8a2) |
CNY 6,840.00 |
|
| MR210118L3 | Lenti ORF clone of Col8a2 (Myc-DDK-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2) |
CNY 8,080.00 |
|
| MR210118L4 | Lenti ORF clone of Col8a2 (mGFP-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2) |
CNY 8,080.00 |
