United States
Germany
Japan
United Kingdom
China
C1s (BC018319) Mouse Tagged ORF Clone
CAT＃: MR210085
- TrueORF®
C1s (Myc-DDK-tagged) - Mouse complement component 1, s subcomponent (cDNA clone MGC:19094 IMAGE:4196654)
ORF Plasmid: tGFP
"BC018319" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,088.00
CNY 6,940.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC37773, C1sa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAATCGCCAGGGATGTGGTGTTTGGTCCTGTTTTCTCTTTTGGCATCATTTTCTGCTGAGCCCA CCATGCATGGGGAGATCCTGTCCCCTAACTATCCCCAGGCGTACCCCAATGACGTCGTGAAATCTTGGGA CATTGAAGTCCCAGAGGGGTTTGGGATTCACCTCTACTTTACCCATGTGGACATAGAGCCGTCAGAGAGC TGTGCATACGACTCAGTGCAGATAATCTCAGGAGGCATCGAGGAAGGGAGACTCTGTGGCCAGAAGACCA GCAAGAGCCCCAACTCCCCCATTATAGAAGAGTTTCAGTTCCCATACAATAAACTTCAGGTGGTCTTTAC CTCAGACTTCTCCAACGAAGAACGGTTTACGGGCTTTGCAGCATACTACACTGCCATAGACATAAATGAA TGCACAGATTTTACAGATGTCCCCTGTAGCCACTTCTGCAATAACTTCATTGGTGGATACTTCTGCTCCT GTCCCCCAGAATACTTCCTCCACGATGACATGAGGAATTGTGGAGTCAACTGTAGTGGAGATGTGTTCAC TGCCCTGATTGGGGAGATCTCAAGTCCCAATTATCCCAATCCATACCCGGAGAACTCAAGGTGTGAATAC CAGATTCAGCTGCAGGAGGGCTTCCAAGTGGTGGTGACGATGCAAAGAGAAGATTTTGATGTGGAGCCTG CTGACTCAGAGGGGAACTGCCCTGACAGTTTAACTTTTGCTTCAAAAAACCAACAGTTTGGTCCTTACTG TGGTAATGGGTTCCCTGGACCGCTAACTATTAGAACCCAGAGCAATACTCTCGGCATTGTCTTTCAAACT GACCTAATGGGGCAAAAGAAGGGTTGGAAGCTTCGTTACCATGGAGATCCCATCTCCTGTGCCAAAAAAA TCACTGCCAATTCTACTTGGGAGCCCGATAAGGCAAAATACGTGTTTAAAGATGTGGTGAAGATAACCTG TGTGGACGGATTTGAAGTTGTGGAGGGACATGTTAGCTCTACTTCATACTATTCCACTTGTCAAAGCGAT GGACAGTGGAGCAACTCCGGTCTAAAATGTCAACCTGTGTATTGTGGAATTCCAGATCCCATTGCGAATG GTAAAGTTGAAGAGCCAGAGAATAGTGTGTTTGGTACTGTTGTTCACTACACGTGTGAAGAGCCATATTA CTACATGGAACATGAAGAAGGTGGGGAGTATCGATGTGCTGCAAATGGGAGATGGGTAAATGACCAGCTG GGTATTGAGCTTCCAAGATGTATTCCAGCCTGTGGAGTACCCACCGAGCCCTTTCAAGTGCATCAGAGGA TATTTGGAGGACAACCTGCAAAGATTGAAAATTTTCCCTGGCAAGTCTTCTTTAATCACCCCAGAGCTAG TGGGGCTCTTATTAATGAGTACTGGGTGCTGACGGCTGCTCACGTTTTGGAGAAAATCTCCGACCCTTTA ATGTATGTCGGGACCATGTCTGTGAGGACAACTCTCTTGGAAAATGCCCAGAGACTCTACAGTAAACGTG TCTTTATTCATCCCAGCTGGAAGAAAGAAGATGACCCAAACACACGGACAAATTTTGACAATGACATTGC CCTGGTGCAGCTGAAAGACCCTGTGAAAATGGGACCCAAAGTCTCCCCCATCTGCCTACCAGGCACCTCC TCAGAGTACAACGTCTCACCGGGTGACATGGGGCTGATCTCAGGGTGGGGCAGTACAGAAAAGAAAGTCT TTGTTATCAACCTCAGAGGGGCAAAGGTACCAGTAACCTCTTTAGAAACCTGCAAACAGGTGAAAGAGGA GAACCCCACTGTAAGGCCTGAGGACTATGTTTTCACTGACAACATGATCTGTGCTGGCGAGAAAGGTGTT GACAGCTGTCACGGGGACAGCGGCGGGGCTTTTGCTTTCCAGGTCCCCAATGTCACGGTCCCCAAATTCT ATGTAGCTGGCTTGGTGTCTTGGGGGAAAAGGTGTGGGACTTATGGGGTTTACACAAAGGTAAAGAATTA CGTGGACTGGATCCTGAAAACTATGCAGGAGAATAGTGGGCCCAGGAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210085 protein sequence
Red=Cloning site Green=Tags(s) MGKSPGMWCLVLFSLLASFSAEPTMHGEILSPNYPQAYPNDVVKSWDIEVPEGFGIHLYFTHVDIEPSES CAYDSVQIISGGIEEGRLCGQKTSKSPNSPIIEEFQFPYNKLQVVFTSDFSNEERFTGFAAYYTAIDINE CTDFTDVPCSHFCNNFIGGYFCSCPPEYFLHDDMRNCGVNCSGDVFTALIGEISSPNYPNPYPENSRCEY QIQLQEGFQVVVTMQREDFDVEPADSEGNCPDSLTFASKNQQFGPYCGNGFPGPLTIRTQSNTLGIVFQT DLMGQKKGWKLRYHGDPISCAKKITANSTWEPDKAKYVFKDVVKITCVDGFEVVEGHVSSTSYYSTCQSD GQWSNSGLKCQPVYCGIPDPIANGKVEEPENSVFGTVVHYTCEEPYYYMEHEEGGEYRCAANGRWVNDQL GIELPRCIPACGVPTEPFQVHQRIFGGQPAKIENFPWQVFFNHPRASGALINEYWVLTAAHVLEKISDPL MYVGTMSVRTTLLENAQRLYSKRVFIHPSWKKEDDPNTRTNFDNDIALVQLKDPVKMGPKVSPICLPGTS SEYNVSPGDMGLISGWGSTEKKVFVINLRGAKVPVTSLETCKQVKEENPTVRPEDYVFTDNMICAGEKGV DSCHGDSGGAFAFQVPNVTVPKFYVAGLVSWGKRCGTYGVYTKVKNYVDWILKTMQENSGPRKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC018319 |
| ORF Size | 2082 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018319.1 |
| RefSeq Size | 2855 bp |
| RefSeq ORF | 2084 bp |
| Locus ID | 50908 |
| MW | 77.4 kDa |
| Gene Summary | C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210085 | C1s (tGFP-tagged) - Mouse complement component 1, s subcomponent (cDNA clone MGC:19094 IMAGE:4196654) |
CNY 7,600.00 |
|
| MR210085L3 | Lenti ORF clone of C1s (Myc-DDK-tagged) - Mouse complement component 1, s subcomponent (cDNA clone MGC:19094 IMAGE:4196654) |
CNY 8,840.00 |
|
| MR210085L4 | Lenti ORF clone of C1s (mGFP-tagged) - Mouse complement component 1, s subcomponent (cDNA clone MGC:19094 IMAGE:4196654) |
CNY 8,840.00 |
