Prmt7 (NM_145404) Mouse Tagged Lenti ORF Clone
CAT#: MR210073L3
- LentiORF®
Lenti ORF clone of Prmt7 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7)
"NM_145404" in other vectors (4)
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CNY 7,700.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4933402B05Rik; BC006705 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR210073).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145404 |
ORF Size | 2079 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145404.1, NP_663379.1 |
RefSeq Size | 2269 bp |
RefSeq ORF | 2079 bp |
Locus ID | 214572 |
UniProt ID | Q922X9 |
Gene Summary | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200516 | Prmt7 (untagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7), (10ug) |
CNY 5,080.00 |
|
MG210073 | Prmt7 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7) |
CNY 6,370.00 |
|
MR210073 | Prmt7 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7) |
CNY 5,080.00 |
|
MR210073L4 | Lenti ORF clone of Prmt7 (mGFP-tagged) - Mouse protein arginine N-methyltransferase 7 (Prmt7) |
CNY 7,700.00 |