United States
Germany
Japan
United Kingdom
China
Fbxl5 (BC047214) Mouse Tagged ORF Clone
CAT＃: MR210023
- TrueORF®
Fbxl5 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511)
ORF Plasmid: tGFP
"BC047214" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fbl4; Fir4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210023 representing BC047214
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAACAACCAGGAGTTAAAATACGAAATAAAATGGAAGCACCTTGACTGCAGTGTCGTCGAGCTTT CTAAAACCAACTTTTCCAACAACAATGACTTCCGTGCTCTTCTCCAGTCTTTATATGCTACTTTCAAGGA GTTTAAAATGCATGAACAAATTGAAAATGAATACATTATCGGTTTACTTCAGCAGCGCAGTCAGACCATA TATAATGTACACTCTGACAATAAGCTCTCCGAGATGCTGAGTCTCTTTGAAAAGGGGCTGAAGAATGTCA AGAATGAGTACGAACAGTTAAATTATGCAAAACAGCTGAAAGAGAGATTGGAGGCTTTTACAAGAGATTT TCTTCCTCACATGAAAGAGGAAGAAGAGGTTTTTCAGCCCATGTTAATGGAATATTTTACCTACGAGGAG CTTAAAGATATCAAAAAGAAAGTGATTGCACAGCACTGCTCACAGAAGGACACGGCAGAGCTCCTCAGAG GGCTCAGTCTGTGGAACCAAGCTGAGGAGCGACAGAAAGTTCTCAAGTACTCTGTGGATGAGAAGGCAGA TACAGAAGCGGAAGTGTCAGAACACTCCACAGGTATAACCCATCTTCCTCCTGAGGTAATGCTGTCCATT TTCAGTTACCTTAATCCTCAAGAATTGTGTCGGTGTAGTCAAGTCAGTACTAAGTGGTCTCAGCTGGCAA AAACAGGATCTTTGTGGAAACATCTTTACCCTGTTCATTGGGCAAGAGGTGACTGGTATAGTGGTCCTGC CACTGAACTTGATACTGAGCCTGATGAGGAATGGGTGAGAAATAGGAAAGATGAAAGTCGTGCTTTCCAG GAGTGGGATGAAGATGCCGATATAGATGAATCTGAAGAGTCTGCAGAGGAATCGGTTGCTATCAGCATTG CACAAATGGAAAAACGTGTACTCCATGGCTTAATTCATAATGTTCTGCCATATGTTGGTACCTCTGTAAA AACTTTAGTGTTAGCGTACAGCTCTGCAGTCTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCT AACCTGGAACATCTGGATCTTACCCAGACCGACATTTCTGATTCTGCATTTGACAGTTGGTCTTGGCTCG GTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGATGTGAAAAAATCACAGACATGGCTCTAGAGAA GATTTCTAGAGCTCTTGGAGTTCTGACATCTCATCAGAGTGGCTTTCTGAAAAGTGCAGGCAAGGCTGCT TCAACTCCATGGACAAGTAAAGACATTACCATGCCATCCACCACGCAGTATGCCTGTTTGCACAATTTAA CTGACAAAGGCATTGGTGAAGAAATAGACAACGAGCACTCTTGGACTGAACCTGTGTCTTCTGAAAGTTT GACTTCTCCTTATGTGTGGATGCTAGATGCTGAAGATTTGGCTGATATTGAAGATGCTGTAGAGTGGAGA CACAGAAATGTTGAGAGTCTCTGTGTGATGGAAACAGCCTCCAACTTTGGTTGTTCCTCATCTGGTTGTT ACAGTAAGGACATTGTGGGATTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCTCCAGCCTTTGC ATATTGTGGTCATTCATTCTGTTGTACAGGGACAGCTTTAAGAACTATGACTACACTCCCAGCGACTTCT GCAATGTGTAGAAAAGCATTAAGGACTACATTGCCCAGGGGGAAAGACTTAATTTATTTTGGGAGTGAAA AGTCTGACCAAGAGACTGGACGAGTACTTTTGTTCCTCAGTCTGTCTGGATGTTACCAGATCACAGACCA TGGTCTCAGGGCTCTGACTCTGGGAGGAGGGCTGCCTTACCTGGAGCACCTGAATCTCTCTGGCTGTCTG ACTGTAACTGGTGCAGGGCTGCAGGATTTGGTCTCAGCGTGTCCTTCCCTAAATGACGAATACTTTTACT ACTGTGACAACATTAACGGTCCTCATGCTGACACCGCCAGTGGATGCCAGAATTTGCAGTGTGGTTTTCG AGCCTGCTGCCGCTCTGGCGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210023 representing BC047214
Red=Cloning site Green=Tags(s) MRNNQELKYEIKWKHLDCSVVELSKTNFSNNNDFRALLQSLYATFKEFKMHEQIENEYIIGLLQQRSQTI YNVHSDNKLSEMLSLFEKGLKNVKNEYEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLMEYFTYEE LKDIKKKVIAQHCSQKDTAELLRGLSLWNQAEERQKVLKYSVDEKADTEAEVSEHSTGITHLPPEVMLSI FSYLNPQELCRCSQVSTKWSQLAKTGSLWKHLYPVHWARGDWYSGPATELDTEPDEEWVRNRKDESRAFQ EWDEDADIDESEESAEESVAISIAQMEKRVLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMVRQILELCP NLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGCEKITDMALEKISRALGVLTSHQSGFLKSAGKAA STPWTSKDITMPSTTQYACLHNLTDKGIGEEIDNEHSWTEPVSSESLTSPYVWMLDAEDLADIEDAVEWR HRNVESLCVMETASNFGCSSSGCYSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMTTLPATS AMCRKALRTTLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRALTLGGGLPYLEHLNLSGCL TVTGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGFRACCRSGE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC047214 |
| ORF Size | 2052 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC047214.1 |
| RefSeq Size | 2778 bp |
| RefSeq ORF | 2054 bp |
| Locus ID | 242960 |
| MW | 101.9 kDa |
| Gene Summary | Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220930 | Fbxl5 (untagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511), (10ug) |
CNY 9,120.00 |
|
| MG210023 | Fbxl5 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511) |
CNY 7,510.00 |
|
| MR210023L3 | Lenti ORF clone of Fbxl5 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511) |
CNY 8,740.00 |
|
| MR210023L4 | Lenti ORF clone of Fbxl5 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 5 (cDNA clone MGC:57022 IMAGE:6466511) |
CNY 8,740.00 |
