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Prkch (NM_008856) Mouse Tagged ORF Clone
CAT＃: MR210017
- TrueORF®
Prkch (Myc-DDK-tagged) - Mouse protein kinase C, eta (Prkch)
ORF Plasmid: tGFP
"NM_008856" in other vectors (4)
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CNY 5,008.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pkc; Pkch |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210017 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGGCACGATGAAGTTCAATGGCTATCTGAGGGTCCGCATCGGAGAGGCTGTAGGGCTGCAGC CCACCCGCTGGTCCCTGCGGCACTCGCTCTTCAAAAAGGGCCACCAGCTGCTGGACCCCTACCTGACGGT GAGCGTAGACCAGGTACGCGTGGGCCAGACCAGCACAAAGCAGAAGACCAACAAACCCACCTACAACGAG GAGTTCTGCGCCAATGTCACCGACGGCGGCCACCTGGAGCTAGCCGTCTTCCACGAGACGCCCCTGGGTT ATGACCACTTTGTGGCCAACTGCACGCTGCAGTTCCAGGAGCTGTTGCGCACGGCTGGTACCTCGGACAC CTTCGAGGGCTGGGTGGATCTGGAGCCTGAGGGGAAAGTGTTTGTGGTAATAACCCTAACAGGGAGTTTC ACTGAAGCCACTCTCCAGAGAGACCGCATCTTCAAGCATTTTACCAGGAAGCGCCAAAGGGCTATGCGAA GACGAGTCCATCAAGTGAACGGACATAAGTTCATGGCCACGTACCTGAGGCAGCCCACCTACTGCTCTCA TTGCCGAGAGTTCATCTGGGGAGTATTTGGGAAACAGGGTTATCAATGCCAAGTGTGCACCTGCGTCGTC CATAAACGCTGCCATCATCTAATTGTTACAGCCTGCACTTGCCAAAACAATATTAACAAAGTGGATGCCA AGATTGCAGAACAGCGGTTTGGCATCAACATCCCACACAAGTTCAACGTTCACAATTACAAGGTGCCCAC GTTCTGTGACCACTGTGGCTCCCTGCTCTGGGGGATAATGCGACAAGGACTTCAGTGTAAAATATGTAAG ATGAATGTACATATTCGGTGTCAGGCGAACGTGGCCCCAAACTGCGGGGTGAATGCCGTGGAGCTTGCCA AGACCCTGGCAGGGATGGGTCTCCAACCCGGAAATATTTCTCCAACCTCGAAACTCATTTCCAGGTCGAC ACTAAGACGGCAGGGAAAGGAGGGCTCCAAAGAAGGAAATGGGATCGGTGTTAACTCTTCCAGCAGATTC GGCATCGACAACTTTGAGTTCATCCGGGTGTTGGGGAAGGGGAGCTTCGGGAAGGTGATGCTTGCCAGGA TAAAGGAGACAGGAGAACTGTACGCCGTGAAGGTGCTGAAGAAGGACGTGATTCTGCAGGATGATGATGT AGAGTGCACCATGACTGAGAAGAGGATCCTGTCCTTGGCTCGCAACCACCCCTTCCTCACCCAGCTCTTC TGCTGCTTTCAGACTCCAGACCGTCTGTTCTTTGTCATGGAGTTTGTGAACGGAGGCGACCTGATGTTCC ACATCCAAAAGTCCCGTCGTTTCGATGAAGCCCGTGCTCGTTTCTACGCCGCGGAGATCATTTCTGCACT CATGTTCCTACATGAGAAAGGTATCATCTATAGAGACTTGAAACTGGACAATGTGCTATTGGACCACGAA GGTCACTGTAAACTGGCCGATTTTGGAATGTGCAAGGAGGGGATTTGTAATGGGGTCACCACAGCCACCT TCTGCGGTACACCTGACTACATTGCCCCAGAGATCCTTCAGGAGATGCTGTATGGACCTGCAGTAGACTG GTGGGCCATGGGCGTGTTGCTTTATGAGATGCTGTGCGGACATGCGCCCTTTGAGGCTGAAAATGAAGAT GACCTTTTTGAGGCCATACTGAATGATGAAGTCGTCTACCCCACCTGGCTCCATGAAGATGCCACAGGGA TCCTCAAGTCTTTCATGACCAAGAACCCCACCATGCGCTTGGGCAGCCTGACTCAGGGAGGAGAGCATGA GATCCTGAGACACCCTTTCTTTAAGGAAATCGACTGGGCCCAGTTGAACCATCGCCAGTTAGAGCCGCCT TTCCGACCTAGAATCAAATCCCGAGAAGATGTCAGCAATTTTGACCCAGACTTTATAAAAGAAGAGCCCG TCTTAACTCCGATTGATGAGGGACATCTTCCTATGATTAACCAGGATGAGTTTAGAAACTTTTCCTATGT GTCACCGGAATTGCAACTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR210017 protein sequence
Red=Cloning site Green=Tags(s) MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNE EFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTAGTSDTFEGWVDLEPEGKVFVVITLTGSF TEATLQRDRIFKHFTRKRQRAMRRRVHQVNGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVV HKRCHHLIVTACTCQNNINKVDAKIAEQRFGINIPHKFNVHNYKVPTFCDHCGSLLWGIMRQGLQCKICK MNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLISRSTLRRQGKEGSKEGNGIGVNSSSRF GIDNFEFIRVLGKGSFGKVMLARIKETGELYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLF CCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHEKGIIYRDLKLDNVLLDHE GHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENED DLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHEILRHPFFKEIDWAQLNHRQLEPP FRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008856 |
| ORF Size | 2052 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008856.2 |
| RefSeq Size | 3301 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 18755 |
| UniProt ID | P23298 |
| MW | 77.9 kDa |
| Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in the human gene are associated with susceptibility to cerebral infarction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220401 | Prkch (untagged) - Mouse protein kinase C, eta (Prkch), (10ug) |
CNY 8,740.00 |
|
| MG210017 | Prkch (tGFP-tagged) - Mouse protein kinase C, eta (Prkch) |
CNY 6,370.00 |
|
| MR210017L3 | Lenti ORF clone of Prkch (Myc-DDK-tagged) - Mouse protein kinase C, eta (Prkch) |
CNY 7,700.00 |
|
| MR210017L4 | Lenti ORF clone of Prkch (mGFP-tagged) - Mouse protein kinase C, eta (Prkch) |
CNY 7,700.00 |
