Tcf25 (NM_001037878) Mouse Tagged ORF Clone
CAT#: MR209961
- TrueORF®
Tcf25 (Myc-DDK-tagged) - Mouse transcription factor 25 (basic helix-loop-helix) (Tcf25), transcript variant 3
"NM_001037878" in other vectors (3)
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CNY 6,750.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1100001J13Rik; 1810041K11Rik; D8Ertd325e; mKIAA1049; Nulp1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209961 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGCCGGGCCCTCCGGAGACTGAGGGGGGAACAGCGCGGCCAGGAACCCCTAGGGCCCGACGCCT TGAAGTTTGTCCTCCTTGATGACGATGACGCAGAGGAAGAGGGACCAAAACCGGGGCTGGGTGGCCGGCG CCCCGGGGGTGCAGGGAAGGAAGGAGTCCGTGTCAACAACCGGTTTGAGCTGATAAACACGGAGGATCTT GAGGATGACCTTGTGGTAAATGGGGAGAGATCTGACTGTACCCTGCCGGACTCTGTGTCATCAGGGAACA AAGGAAGGGCTAAACATGGAAACGCAGAGACCAAGCAAGATGGAGGGGCCACCAAAGCAGGGTCCTCGGA ACAGTCTAATGCAAGTGGCAAACTCCGAAAGAAGAAAAAGAAGCAGAAAAATAAGAAAAGCTGCACAGGA GAATCCTCGGAGAATGGACTAGAAGACATCGATCGCATCTTAGAGCGGATTGAGGACAGCAGTGGGTTCA GCCACCCCGGCCCCCCTCCACTGAGCTCCAGGAAGCACGTCCTATATGTGGAGCACAGACACCTGAATCC AGACACGGAACTGAAGAGGTATTTTGGTGCTCGAGCGGTCCTGGGCGAGCAAAGGCCTCGACAGAGGCAG CGTGTGTATCCCAAGTGTACATGGCTGACGACCCCTAAGAGCACCTGGCCCCGGTACAGCAAACCAGGTC TATCAATGCGGCTGCTGGAGTCCAAGAAAGGCCTTTCTTTCTTTGCATTTGACCATAATGAAGAATACCA ACAAGCACAGCACAAGTTCCTGGTGGCTGTGGAGTCCATGGAGCCAAATAACATTGTGGTTCTGCTCCAG ACAAGCCCCTATCATGTGGACTCCCTGCTGCAGCTCAGTGATGCCTGCCGCTTTCAAGAGGATCAAGAGA TGGCTCGAGACCTTATAGAGAGAGCCCTGTACAGCATGGAGTGTGCATTCCACCCCTTGTTCAGTCTCAC CAGTGGAACTTGCAGGCTGGATTATCGCAGACCTGAGAATAGGAGCTTCTACCTGACCCTCTACAAGCAG ATGAGTTTCTTGGAGAAACGTGGATGCCCACGTACTGCGCTGGAGTACTGCAAGCTCATCCTGAGCCTTG AGCCAGATGAGGACCCCCTGTGCATGTTGCTGCTTATTGACCACCTGGCCTTACGGGCCCGGAACTATGA ATACCTGATTCGCCTCTTCCAGGAATGGGAGGCTCATCGGAACCTGTCCCAGCTCCCAAATTTTGCCTTC TCAGTTCCACTGGCCTATTTCCTTCTGAGTCAGCAAACAGACTTACCTGAGCATGAGCTCAGCTCTGCCA GGCAACAGGCTTCCCTCCTGATACAGCAGGCACTCACCATGTTCCCTGGAGTTCTTATGCCACTGCTGGA GTATTGCAGTGTGCGACCTGATGCCACGGTCTCAAACCACCGCTTCTTTGGGCCGGATGCTGAGATCAGC CAGCCTCCCGCCTTGGGCCAGCTGGTGAGTCTGTACCTGGGGAGGTCACACTTTCTTTGGAAAGAACCCG CCATTATGAGCTGGCTAGAGGAAAATGTCCATGAGGTTCTGCAAGCGGTGGATGCTGGAGACCCTGCTGT GGAGGCCTGTGAGAACAGGCGGAAAGTGTTGTACCAGCGTGCGCCCAGAAACATCCACCGCCACGTGATA CTGTCTGAGATCAAGGAGGCTGTTGCTGCCCTACCCTCGGATGTGACCACACAGTCTGTGATGGGGTTCG ACCCTCTGCCTCCCCTGGACACCATCTACTCTTACGTCAGACCAGAGAGGCTGAGTCCAGTCAGCCACGG AAACACAATCGCCCTCTTCTTCCGGTCCTTGTTGCCAAATTACACCACAGAGGGGGAGAGGCTGGAGGAA GGAGTGGCCGGGGGTCCGAACCGCAATCAAGGCTTGAACAGGCTGATGTTGGCCGTACGTGACATGATGG CCAACTTCCACTTCAACGACCTGGAGGTGCCTCGGGAGGACAATCCTGAGGGCGAGGGGGACTGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209961 protein sequence
Red=Cloning site Green=Tags(s) MSRRALRRLRGEQRGQEPLGPDALKFVLLDDDDAEEEGPKPGLGGRRPGGAGKEGVRVNNRFELINTEDL EDDLVVNGERSDCTLPDSVSSGNKGRAKHGNAETKQDGGATKAGSSEQSNASGKLRKKKKKQKNKKSCTG ESSENGLEDIDRILERIEDSSGFSHPGPPPLSSRKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQ RVYPKCTWLTTPKSTWPRYSKPGLSMRLLESKKGLSFFAFDHNEEYQQAQHKFLVAVESMEPNNIVVLLQ TSPYHVDSLLQLSDACRFQEDQEMARDLIERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLTLYKQ MSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARNYEYLIRLFQEWEAHRNLSQLPNFAF SVPLAYFLLSQQTDLPEHELSSARQQASLLIQQALTMFPGVLMPLLEYCSVRPDATVSNHRFFGPDAEIS QPPALGQLVSLYLGRSHFLWKEPAIMSWLEENVHEVLQAVDAGDPAVEACENRRKVLYQRAPRNIHRHVI LSEIKEAVAALPSDVTTQSVMGFDPLPPLDTIYSYVRPERLSPVSHGNTIALFFRSLLPNYTTEGERLEE GVAGGPNRNQGLNRLMLAVRDMMANFHFNDLEVPREDNPEGEGDWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001037878 |
ORF Size | 2028 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001037878.4 |
RefSeq Size | 2306 bp |
RefSeq ORF | 2031 bp |
Locus ID | 66855 |
UniProt ID | Q8R3L2 |
MW | 76.7 kDa |
Gene Summary | May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204165 | Tcf25 (untagged) - Mouse transcription factor 25 (basic helix-loop-helix) (Tcf25), transcript variant 3, (10ug) |
CNY 4,960.00 |
|
MR209961L3 | Lenti ORF clone of Tcf25 (Myc-DDK-tagged) - Mouse transcription factor 25 (basic helix-loop-helix) (Tcf25), transcript variant 3 |
CNY 8,650.00 |
|
MR209961L4 | Lenti ORF clone of Tcf25 (mGFP-tagged) - Mouse transcription factor 25 (basic helix-loop-helix) (Tcf25), transcript variant 3 |
CNY 8,650.00 |