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Clcnkb (BC019983) Mouse Tagged ORF Clone
CAT＃: MR209960
- TrueORF®
Clcnkb (Myc-DDK-tagged) - Mouse chloride channel Kb (cDNA clone MGC:28502 IMAGE:4188062)
ORF Plasmid: tGFP
"BC019983" in other vectors (4)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Clc-Ka, CLCKB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209960 representing BC019983
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAATTAGTGGGGCTTCGTGAGGGCGCCTCCAAGAAGCCAGTGCCACTGCAAGAACTCTGGGGGC CATGCCCACGTATCCGCAGAAACATCCAGGGGGGCCTAGAGTGGCTGAAAGAGCGGCTCTTCCGTGTGGG TGAGGACTGGTACTTCCTGGTGGCTCTCGGGGTGCTCATGGCTCTAATCAGCTATGCTATGAACTTCACT ATTGGGCGCGTGGTCAGAGCACACAAGTGGCTGTACAGGGAGATTGGAGACGGCCACTTGCTCCTGTACC TCTCCTGGACCGTGTACCCCGTGGCTCTTCTGTCCTTCTCCTCCGGCTTCTCACAGAGCATTACGCCATC CTCTGGAGGGTCTGGAATCCCTGAGGTGAAGACCATCTTGACCGGAGTGGTCCTGGAGGACTACCTAGAC ATCAAGAACTTTGGGGCCAAGGTGGTGGGCCTCTCCTGCACCCTGGCCACAGGCAGTACCATCTTCCTGG GAAAACTGGGCCCCTTCGTGCACCTGAGCGTGATGATCGCTGCTTACCTGGGCCGCGTGCGCACCAAGAC CGTCGGGGAACCTGAGAGCAAGACCAAAGAAATGGAACTGTTGGCCGCAGGAGCAGCAGTGGGTGTGGCC ACGGTCTTCGCAGCTCCCATCAGCGGTGTCCTGTTCAGCATCGAGGTCATGTCCTCTCACTTCTCCGTCT GGGATTACTGGAGGGGCTTCTTCGCTGCCACCTGTGGGGCCTTTATGTTCCACCTCCTGGCGGTCTTCAA CAATGAACAGGAGACCATCACCTCCATCTACAAGACCAGCTTTCCAGTGGACATACCCTTTGACTTACCA GAGATCTTCTTTTTTGTGGCCCTGGGGGCCATCTGTGGCATCCTGAGCTGCGGGTACAACTACAGCCAGC GGACTTTCTTATTTTTCCTCAAGGCCAATGGGTTCACCTCCAAACTGCTGGCTACCAGCAAACCCTTGTA CTCAGCCCTGGCTGCTGTGGTCCTGGCTTCCATCACGTATCCCCCTGGTGTGGGCCACTTCATGGCTTCC CGGCTGTCCATGTCGGAGCATTTGGAGACACTGTTTGACAACAACTCCTGGGCACTGATGACTAAGAACT CGTCTCCACCCTGGGCAGCGGAGCCTGATCCCCAGAAGCTGTGGTTGGAGTGGTGTCACCCACAGTTGAC TGTCTTTGGGACACTAGTCTTCTTCCTGGTCATGAAGTTCTGGATGCTGATTTTGGCAACCACCATCCCC ATCCCTGCTGGATACTTCTTGCCCATCTTTGTCTATGGAGCTGTCATTGGGCGCCTCTTTGGGGAGGTTC TATCTGTCGCCTTCCCAGAGGGCATTGTGGCTGGAGGCAGGGTCAATCCCATCATGCCCGGGGCCTATGC TCTGGCAGGTGCTGCTGCCTTCTCGGGGGCGGTGACCCACACCCTCTCCACGGCACTGCTGGCCTTTGAG GTAACGGGCCAGCTCGTTCACGCACTGCCTGTGCTGATGGCTGTGCTGGCGGCCAACGCCATCTCTCAGA GCTTCCAGCCCTCTTTCTACGATGGCACCATCATTGTCAAGAAACTGCCGTACCTGCCTTGGATCCGTGG CCGACAGATTGGTTCCCACTCTGTAACTGTGGGTCACTTCATGAACTGCACCCTCACTACACTGGCCAAG GACATGCCCCTGGAGCAAGTGATCCAAGTCATGATCTCCACAGATGTGACCCAGTACCCCCTGGTGGAGA CTACAGAGTCTCAGACCCTGGTGGGCGTTGTAAAAAGGACCCACTTGGTGCAAGCCCTCCAGACTGAGCC AGCTTCCTGGGCTCCAGGCCAGCAGCCCTATCTCCAGGATATCTTGGCTAATGGCTGCCCCACCCAGCCA GTGACACTGCAGCTGTCCCCGGAGACCTCCTTGCATGAGACACACAACCTTTTTGAGCTGCTGAACCTTC AGACGCTGTTCGTGACGTCACGAGGCAGAGCTGTGGGCTTTGTATCGTGGGTGGAGGTACTGGGGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209960 representing BC019983
Red=Cloning site Green=Tags(s) MEELVGLREGASKKPVPLQELWGPCPRIRRNIQGGLEWLKERLFRVGEDWYFLVALGVLMALISYAMNFT IGRVVRAHKWLYREIGDGHLLLYLSWTVYPVALLSFSSGFSQSITPSSGGSGIPEVKTILTGVVLEDYLD IKNFGAKVVGLSCTLATGSTIFLGKLGPFVHLSVMIAAYLGRVRTKTVGEPESKTKEMELLAAGAAVGVA TVFAAPISGVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFHLLAVFNNEQETITSIYKTSFPVDIPFDLP EIFFFVALGAICGILSCGYNYSQRTFLFFLKANGFTSKLLATSKPLYSALAAVVLASITYPPGVGHFMAS RLSMSEHLETLFDNNSWALMTKNSSPPWAAEPDPQKLWLEWCHPQLTVFGTLVFFLVMKFWMLILATTIP IPAGYFLPIFVYGAVIGRLFGEVLSVAFPEGIVAGGRVNPIMPGAYALAGAAAFSGAVTHTLSTALLAFE VTGQLVHALPVLMAVLAANAISQSFQPSFYDGTIIVKKLPYLPWIRGRQIGSHSVTVGHFMNCTLTTLAK DMPLEQVIQVMISTDVTQYPLVETTESQTLVGVVKRTHLVQALQTEPASWAPGQQPYLQDILANGCPTQP VTLQLSPETSLHETHNLFELLNLQTLFVTSRGRAVGFVSWVEVLGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC019983 |
| ORF Size | 2028 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019983.1 |
| RefSeq Size | 2534 bp |
| RefSeq ORF | 2030 bp |
| Locus ID | 56365 |
| MW | 92.8 kDa |
| Gene Summary | This gene is a member of the CLC family of voltage-gated chloride channels. The gene is located adjacent to a highly similar chloride channel gene on chromosome 4. This gene is syntenic with human CLCNKA (geneID:1187). [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220797 | Clcnkb (untagged) - Mouse chloride channel Kb (cDNA clone MGC:28502 IMAGE:4188062), (10ug) |
CNY 9,120.00 |
|
| MG209960 | Clcnkb (tGFP-tagged) - Mouse chloride channel Kb (cDNA clone MGC:28502 IMAGE:4188062) |
CNY 7,410.00 |
|
| MR209960L3 | Lenti ORF clone of Clcnkb (Myc-DDK-tagged) - Mouse chloride channel Kb (cDNA clone MGC:28502 IMAGE:4188062) |
CNY 8,650.00 |
|
| MR209960L4 | Lenti ORF clone of Clcnkb (mGFP-tagged) - Mouse chloride channel Kb (cDNA clone MGC:28502 IMAGE:4188062) |
CNY 8,650.00 |
