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Cast (BC010603) Mouse Tagged ORF Clone
CAT＃: MR209946
- TrueORF®
Cast (Myc-DDK-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078)
ORF Plasmid: tGFP
"BC010603" in other vectors (4)
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CNY 4,936.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209946 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACCACAGAGACTAAGGCTGTGAAAACAGAGTCTAAGAAACCACAATCAAGTGAGCAACCTGTGG TTCACGAGAAAAAGTCAAAAGGAGGGCCAAAGGAAGGGTCAGAGCCCAAAAATCTTCCGAAGCACACATC AAGTACAGGAAGCAAGCATGCTCATAAAGAAAAAGCACTTTCCAGATCAAATGAACAGATGGTGTCAGAG AAACCATCAGAATCAAAGACAAAATTTCAAGATGTTCCTTCAGCTGGTGGAGAGAGTGTGGCTGGAGGTG GGACTGTGGCCACTGCCTTGGACAAAGTGGTTGGTAAGAAAAAAGAACAGAAACCATTCACACCAGCTTC ACCTGTGCAGTCCACACCGAGCAAGCCATCTGACAAGTCAGGCATGGATGCTGCCTTGGATGACCTGATA GACACCTTAGGTGGACATGAAGATACTAACAGAGATGATCCACCATATACTGGACCAGTAGTTTTGGACC CAATGTATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACT TTTGGAAAAAAATGAAGGGATCACACAACCTCTTCCAGACTCTCCTAAACCTATGGGGACTGACCAGGCT ATAGATGCCTTGTCATCTGATTTCACCTGTAGTTCTCCAACTGGAAAGCAAAGTGAGAAAGAGAAGTCTA CTGGGGAAATTTTCAAAGCTCAGTCTGCAGGAGTGACCAGAAGTTCTGTTCCCCCCAAGGAGAAGAAAAG GAAGGTGGAAGAGGAGGTCATAAATGATCAAGCACTTCAGGCTCTGTCAGATTCACTGGGCACCCGGCAG CCAGATCCTCCGAGCCACGTTAGCCAAGCTGAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGG AGAAGTGTGGTGAAGATGAGGACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGG AAAACCACTATTGCCAGAGCCTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTT TCAGCAGATTTTGACCGATCTACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACA CATCCCAAACTCCTCCGGGGGAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAA ACTAGCATCCTTGAAGGGCGTGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGG GAAGCAGATCCAGAACATGAAAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATG AAAAACTTGGTGAAAAAGAAGAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGG AAAACCACTCCTGCCCAAAGAATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCC TTGTCTCAGGACTTCTCCAGTCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTG CCATTTCTGAAGTAGTTTCTCAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGA GCAGAAAGACAAAGAACTTGATGATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGAT CCAGATGAGAACAAACCACTGGATGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGG GAGAAAGAGACGACACCATCCCCCCTGAATACAGGCATCTCTTGGATAATGATGGGAAGAAACCTGATCA GGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAACTACAGAGACCTCAAAG AATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGACGGAGAGAAAACAAAGG ACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAAGACACAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209946 protein sequence
Red=Cloning site Green=Tags(s) MSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSE KPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLI DTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQA IDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQ PDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGEL SADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTR EADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDA LSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPD PDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKKPDQDRDPIDALSEDLDSCPSTTETSK NTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010603 |
| ORF Size | 2022 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010603, AAH10603 |
| RefSeq Size | 2712 bp |
| RefSeq ORF | 2024 bp |
| Locus ID | 12380 |
| MW | 73 kDa |
| Gene Summary | This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Calpains Are Downstream Effectors of bax-Dependent Excitotoxic Apoptosis
,Beatrice D'Orsi, Helena Bonner, Liam P. Tuffy, Heiko Düssmann, Ina Woods, Michael J. Courtney, Manus W. Ward, and Jochen H. M. Prehn,
J. Neurosci., Feb 2012; 32: 1847 - 1858.
[CAST]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200735 | Cast (untagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078), (10ug) |
CNY 4,944.00 |
|
| MG209946 | Cast (tGFP-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078) |
CNY 6,536.00 |
|
| MR209946L3 | Lenti ORF clone of Cast (Myc-DDK-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078) |
CNY 5,700.00 |
|
| MR209946L4 | Lenti ORF clone of Cast (mGFP-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078) |
CNY 5,700.00 |
