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Hjurp (NM_198652) Mouse Tagged ORF Clone
CAT＃: MR209893
- TrueORF®
Hjurp (Myc-DDK-tagged) - Mouse Holliday junction recognition protein (Hjurp)
"NM_198652" in other vectors (3)
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CNY 4,896.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6430706D22Rik; A730008H23Rik; C330011F01Rik; Gm975; Mfleg1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209893 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTATGGGTCGGCAGGACCGCAGGCTGCATCAGCAGCTTAAGGAAAGCAGTAGCCGCTTCCAGA CGTTAATGAAGCGGCTGATAGCGAAGTACAACCAGCCCTTTGAGGACGACCCGTTGGTGGAGATGAGGAC GTTAACCTACGAGACGCCCCAGGGGTTGAGAGTTTGGGGAGGAAAGCTGATGAAGAAGGAAGACAAGGAA TACACTCAGGTGATTGACAGATTAAATGGGCAAGCTCCAGAAGGTGATTCAGAGAGCAGCGGTGCGGATA CGAGCTTAGAGGAGAACTGGCCATCTTGCAGCTCAGCGATGAGAGAAGCCTCTGGTGATCCTAGGCAGCG TCAGCCTGCAGTGCCCGGAAACACCTTGGAAACTGATTTAAGAAGGAAGTACTTGACCCAGGTGGATATA TTGCCTCAAGATGAAGAGTATTTTAAGAACGCAGAGAAGAGAGGTGGAAAAGACACAGTCATGACTTGGG TTCCTTCCGTGACCTCATCTGTCACACCAGCCTCTGGATGCCAGGATGCTATCTCTGCAAAGAGCTCTGG TGGTCCTGAAGTATCTGCTTTGTCCTCTAGAGGCCAGGGTCCCTCATACCCTTGCCCAGCTGATATGGCC ATTGTGGCTAGAAGTGATGGCCTCTCCTTACTTGGGACCAGCAGCAACAGCGTAAGCAGCCAGTCCTTTG AGGTTGATGACCTTTGCAATGTGACAATCAGCGATTTGTATGAAGGCATGATGCATTCCATGAGTAGGCT GCTGCGCTCAAAGCCATCCTGCATCATCTCCACCAAGACCTACATCAACCAGAGCTGGAAGCTCCGGAGG AGACCCTCACGCAAGCAGGGGCTGCACAAGAACAGGACACACTGCCCCCGGAGCAAGCCCTCCCAGAGGA GTGCCAGGAAGGGGCCTGCGTCCTGCTCAGAGCCCGGGAAAGAGGCCGGAATATTAAGAGACTACGGGAA TTTACTGCACGTTGCTCCCCACAAGACAGGCTTAGAACTGAAAAGCGTTTCTCTTGAAGGAAGCAAACGC CAAGTCCATAAATCCAGTCCAGCTTGGAAGGAGCTCCAGATGATGCCGCAGAAGGACTTGGACTTAAACC GTGAGCGAGAAAATAGGGTGATGACATTACAGTGGCTAATTTCACCCGTCAAGGTGGTTCCCAGACCAAG GATGCTTCCAAGTCAAGTAGAGAAGTGGTATAGAGAGATTAAAATCAAATTTGACAAGCTTCATCAGGAA TATTGCCTGTCTTCTGGGAAACAGCCTCGCCTGACTGACCCCACAGAGTCTTGGGCTGTCGATGTGTACA GAAGTGGTTCTAAGAGCCCAGGCAGCCGTCAGGATGTAGAAACCTGCAGGCCCAGTTCACCTTTCGGCAG AGAAAAGACAGAAAGGCCAGGTGAAGCTTTAGAAGACCTGAGGGGAAATGGAAAGTCTGTCAAAACAAAG AGCTGTCTTCTAAGGAGCTGTCCCTCTCCAGAGGGCAGCCCGTCACGGAGCCCCAGCCATTCTCAGCTGA GCTCTGGCCTTCAAGAGCACAATTCTGAACCTACTGGTAAGGCAGTGTGGCCCAGTACAGCTATTTCAGC ACCAAGCATAGGCTCTCCAGGCTGTGGGAAGGATAACTATTATGAGCTTAAAAAAGAATTCAACAGACTT TATCAGAAGTATTGTCTGTCACCGCAGCGGGCGAAGGTGACTTCGTGTGGCAGAGTATCGCCAATGAAAG CTGCTGCAGCTCTTCCCTGTCAATCAGAGCACTTGAAGAGACTAAATCCAGACTCCCCACAACAGAGTTC CCAGAAACGGTCAATCTCTCCTGGCTGTCACAGAAGAGTCCTGCAGGATTCAACTGCACAGACTGCTAGT ACACTTGTGAGAGACTCTTGGCTGCCAACAAAGAGGTGCAAGTTGTCATACCCGGTAGCTTGTGCTCACC AGGCCAAGTTTCACGATACCTCAGGAGCATCAGGCTGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209893 protein sequence
Red=Cloning site Green=Tags(s) MESMGRQDRRLHQQLKESSSRFQTLMKRLIAKYNQPFEDDPLVEMRTLTYETPQGLRVWGGKLMKKEDKE YTQVIDRLNGQAPEGDSESSGADTSLEENWPSCSSAMREASGDPRQRQPAVPGNTLETDLRRKYLTQVDI LPQDEEYFKNAEKRGGKDTVMTWVPSVTSSVTPASGCQDAISAKSSGGPEVSALSSRGQGPSYPCPADMA IVARSDGLSLLGTSSNSVSSQSFEVDDLCNVTISDLYEGMMHSMSRLLRSKPSCIISTKTYINQSWKLRR RPSRKQGLHKNRTHCPRSKPSQRSARKGPASCSEPGKEAGILRDYGNLLHVAPHKTGLELKSVSLEGSKR QVHKSSPAWKELQMMPQKDLDLNRERENRVMTLQWLISPVKVVPRPRMLPSQVEKWYREIKIKFDKLHQE YCLSSGKQPRLTDPTESWAVDVYRSGSKSPGSRQDVETCRPSSPFGREKTERPGEALEDLRGNGKSVKTK SCLLRSCPSPEGSPSRSPSHSQLSSGLQEHNSEPTGKAVWPSTAISAPSIGSPGCGKDNYYELKKEFNRL YQKYCLSPQRAKVTSCGRVSPMKAAAALPCQSEHLKRLNPDSPQQSSQKRSISPGCHRRVLQDSTAQTAS TLVRDSWLPTKRCKLSYPVACAHQAKFHDTSGASGWP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198652 |
| ORF Size | 2004 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198652.1 |
| RefSeq Size | 8449 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 381280 |
| UniProt ID | Q6PG16 |
| MW | 74.2 kDa |
| Gene Summary | Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220297 | Hjurp (untagged) - Mouse Holliday junction recognition protein (Hjurp), (10ug) |
CNY 8,550.00 |
|
| MR209893L3 | Lenti ORF clone of Hjurp (Myc-DDK-tagged) - Mouse Holliday junction recognition protein (Hjurp) |
CNY 7,700.00 |
|
| MR209893L4 | Lenti ORF clone of Hjurp (mGFP-tagged) - Mouse Holliday junction recognition protein (Hjurp) |
CNY 7,700.00 |
