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D14Ertd231e (BC055700) Mouse Tagged ORF Clone
CAT＃: MR209823
- TrueORF®
D14Ertd231e (Myc-DDK-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816)
ORF Plasmid: tGFP
"BC055700" in other vectors (4)
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CNY 4,896.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC028953; D14Ertd231e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209823 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTGTATTGCCTGTCAGGGCACCCAACTTTACCATGCAACGTACTCAAATTCAAATCCACTACTA TTATGTTGGACTGTGGACTGGACATGACTTCCACCCTCAATTTCCTCCCTTTGCCTCTTGTTCAAAGCCC CAGGCTATCTAATCTTCCCGGATGGTCTCTAAAAGACGGAAATGCTTTTTTGGACAAGGAGCTAAAAGAG TGCTCCGGTCATGTATTTGTGGATTCTGTGCCTGAATTCTGCTTACCAGAGACCGAGCTAATAGACCTGT CTACAGTCGATGTGATCCTCATCTCTAACTACCACTGCATGATGGCGCTGCCCTACATCACCGAGCACAC AGGATTCACAGGCACAGTGTATGCCACAGAACCCACCATGCAGATCGGCAGGCTTCTCATGGAAGAGCTG GTGAATTTCATCGAAAGAGTGCCAAAGGCCCAGTCTGCCTCCTTGTGGAAGAATAAGGATATACAGCGGC TGCTGCCTTCTCCCCTCAAGGATGCAGTGGAAGTGTCAACCTGGAGAAGATGCTACACGATGCAGGAGGT GAACTCGGCCCTCAGTAAAATCCAGCTGGTGGGATATTCCCAGAAAATCGAGCTGTTTGGTGCTGTCCAG GTGACCCCTCTGAGCTCTGGCTATGCCCTTGGAAGCTCCAACTGGATTATCCAGTCACATTATGAGAAAG TATCTTACGTCTCTGGATCCTCCTTGCTTACCACACACCCTCAGCCCATGGACCAAGCTTCTCTCAAAAA CAGTGACGTTCTGATTCTCACAGGGCTGACACAGATCCCTACTGCAAACCCGGACGGCATGGTGGGCGAG TTCTGCAGCAACCTCGCTCTGACAGTTCGGAATGGAGGAAACGTGTTGGTCCCTTGCTATCCTTCAGGCG TGATCTATGACCTCCTGGAGTGCTTATATCAATACATTGACTCCGCTGGCCTCTCCAACATCCCTTTCTA CTTCATCTCCCCCGTTGCCAACAGTTCACTCGAGTTTTCCCAGATTTTTGCTGAGTGGCTTTGTCACAAC AAACAGAGTAAAGTTTACCTCCCAGAACCACCTTTCCCACATGCAGAGCTCATTCAGACGAATAAGCTGA AGCACTACCGCAGCATCCACGGTGACTTCAGCAATGACTTCAGACAGCCGTGTGTGCTGTTCACCGGACA TCCTTCCCTCCGCTTTGGGGACGTCGTCCACTTCATGGAGCTCTGGGGGAAGTCCAGCCTCAACACCATC ATATTCACAGAACCCGATTTCTCCTACCTGGAGGCCTTGGCTCCTTACCAGCCGCTGGCCATGAAGTGCA TCTACTGCCCAATTGACACTCGACTGAATTTCATCCAGGTGTCAAAGCTGCTTAAAGAAGTACAGCCCCT GCACGTGGTCTGTCCTGAGCAGTACACCCAGCCACCGCCAGCCCAGGCCCACAGGATGGACCTCATGATC GACTGCCAGCCACCAGCCATGTCCTATCGACGGGCTGAGGTCCTTGCCCTGCCCTTCAAACGACGCTATG AGAAAATTGAAATCATGCCAGAGCTGGCAGACTCCCTGGTGCCCATGGAAATCAAGCCTGGCATCTCCTT GGCGACTGTCTCAGCTGTGCTGCATACGAAGGATAACAAACATGTGCTTCAGCCCCCTCCCAAGCCCACC CAGCCCACCAGCAGTAAGAAGAGGAAGCGGGTGAACGAGGACATTCCTGACTGCAAAGTACTGAAGCCTC TGCTGAGTGGCTCCATCCCCGTAGAGCAGTTTGTGCAGACCCTGGAGAAGCATGGCTTCAGTGACATTAA GGTGGAAGACACAGCCAAGGGACATATTGTCCTGCTGCAGGAAGCCGAGACACTCATCCAGATTGAAGAA GACTCCACTCACATCATCTGTGACAATGACGAGACACTGAGGGTCCGACTGCGGGACCTTGTCCTCAGGT TCCTGCAGAAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209823 protein sequence
Red=Cloning site Green=Tags(s) MKLYCLSGHPTLPCNVLKFKSTTIMLDCGLDMTSTLNFLPLPLVQSPRLSNLPGWSLKDGNAFLDKELKE CSGHVFVDSVPEFCLPETELIDLSTVDVILISNYHCMMALPYITEHTGFTGTVYATEPTMQIGRLLMEEL VNFIERVPKAQSASLWKNKDIQRLLPSPLKDAVEVSTWRRCYTMQEVNSALSKIQLVGYSQKIELFGAVQ VTPLSSGYALGSSNWIIQSHYEKVSYVSGSSLLTTHPQPMDQASLKNSDVLILTGLTQIPTANPDGMVGE FCSNLALTVRNGGNVLVPCYPSGVIYDLLECLYQYIDSAGLSNIPFYFISPVANSSLEFSQIFAEWLCHN KQSKVYLPEPPFPHAELIQTNKLKHYRSIHGDFSNDFRQPCVLFTGHPSLRFGDVVHFMELWGKSSLNTI IFTEPDFSYLEALAPYQPLAMKCIYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPPAQAHRMDLMI DCQPPAMSYRRAEVLALPFKRRYEKIEIMPELADSLVPMEIKPGISLATVSAVLHTKDNKHVLQPPPKPT QPTSSKKRKRVNEDIPDCKVLKPLLSGSIPVEQFVQTLEKHGFSDIKVEDTAKGHIVLLQEAETLIQIEE DSTHIICDNDETLRVRLRDLVLRFLQKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC055700 |
| ORF Size | 1974 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055700, AAH55700 |
| RefSeq Size | 2414 bp |
| RefSeq ORF | 1976 bp |
| Locus ID | 210925 |
| MW | 74.1 kDa |
| Gene Summary | Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217972 | D14Ertd231e (untagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816), (10ug) |
CNY 6,940.00 |
|
| MG209823 | D14Ertd231e (tGFP-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816) |
CNY 4,090.00 |
|
| MR209823L3 | Lenti ORF clone of D14Ertd231e (Myc-DDK-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816) |
CNY 5,610.00 |
|
| MR209823L4 | Lenti ORF clone of D14Ertd231e (mGFP-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816) |
CNY 5,610.00 |
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