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Flrt3 (NM_001172160) Mouse Tagged ORF Clone
CAT＃: MR209757
- TrueORF®
Flrt3 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3), transcript variant 1
"NM_001172160" in other vectors (2)
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CNY 4,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5530600M07Rik; C430047I10Rik; mKIAA1469 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209757 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGCCCAGCCTGGAGCCTCTTCCTCATCGGGACTAAAATTGGGCTGTTCTTCCAAGTGGCACCTC TGTCAGTTGTGGCTAAATCCTGTCCATCTGTATGTCGCTGTGACGCAGGCTTCATTTACTGTAACGATCG CTCTCTGACATCCATTCCAGTGGGAATTCCGGAGGATGCTACAACACTCTACCTTCAGAACAACCAAATA AACAATGTTGGGATTCCTTCCGATTTGAAGAACTTGCTGAAAGTACAAAGAATATACCTATACCACAACA GTTTAGATGAATTCCCTACCAACCTTCCAAAGTATGTCAAAGAGTTACATTTGCAAGAGAATAACATAAG GACTATCACCTATGATTCACTTTCGAAAATTCCGTATCTGGAAGAGTTACACTTGGATGATAACTCAGTC TCGGCTGTTAGCATCGAAGAGGGAGCATTTCGAGACAGTAACTATCTGCGGCTGCTTTTTCTGTCCCGTA ACCACCTTAGCACAATCCCGGGGGGCTTGCCCAGGACTATTGAGGAATTACGCCTGGATGACAATCGCAT ATCAACGATCTCTTCCCCATCACTTCATGGTCTCACAAGCCTGAAACGCCTGGTTTTAGATGGAAACTTG TTGAACAACCATGGTTTGGGTGACAAAGTTTTCTTCAACTTAGTAAACTTAACAGAGCTGTCCCTGGTGA GGAATTCCTTGACAGCAGCGCCAGTGAACCTTCCCGGCACAAGCCTGAGGAAGCTTTACCTTCAAGACAA CCATATCAACCGGGTACCCCCAAATGCTTTTTCTTATTTAAGGCAGCTGTATCGACTCGATATGTCTAAT AATAACCTAAGCAATTTACCTCAGGGTATCTTTGATGATTTGGACAATATAACCCAACTGATTCTTCGCA ACAATCCTTGGTATTGTGGATGCAAGATGAAATGGGTACGAGACTGGTTACAGTCGCTACCGGTGAAGGT CAATGTGCGTGGGCTCATGTGCCAAGCCCCAGAAAAGGTCCGTGGAATGGCTATCAAGGACCTCAGTGCA GAACTGTTTGATTGTAAAGACAGTGGGATTGTGAGCACCATTCAGATAACCACTGCAATACCCAACACAG CATATCCTGCTCAAGGACAGTGGCCAGCTCCTGTGACCAAACAACCAGATATTAAAAACCCCAAGCTCAT TAAGGATCAGCGAACTACAGGCAGCCCCTCACGGAAAACAATTTTAATTACTGTGAAATCTGTCACCCCT GACACAATCCACATATCCTGGAGACTTGCTCTGCCTATGACTGCTCTGCGACTCAGCTGGCTTAAACTGG GCCATAGCCCAGCCTTTGGATCTATAACAGAAACAATCGTAACAGGAGAACGCAGTGAATACTTGGTCAC CGCCCTAGAACCTGAATCACCCTATAGAGTATGCATGGTTCCCATGGAAACCAGTAACCTTTACCTGTTT GATGAAACACCTGTTTGTATTGAGACCCAAACTGCCCCTCTTCGAATGTACAACCCCACAACCACCCTCA ATCGAGAGCAAGAGAAAGAACCTTACAAAAATCCAAATTTACCTTTGGCTGCCATCATTGGTGGGGCTGT GGCCCTGGTAAGCATCGCCCTCCTTGCTTTGGTGTGTTGGTATGTGCATAGGAACGGGTCACTGTTTTCA CGGAACTGTGCGTACAGCAAAGGGCGGAGGAGAAAGGATGACTATGCAGAAGCCGGTACTAAGAAAGACA ACTCCATCCTGGAAATCAGGGAAACTTCTTTCCAGATGCTACCGATAAGCAATGAACCCATCTCCAAGGA GGAGTTTGTAATACACACCATATTTCCTCCGAATGGGATGAATCTGTACAAGAACAACCTCAGTGAGAGC AGTAGTAACCGGAGCTACAGAGACAGTGGCATCCCAGACTCGGACCACTCACACTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209757 protein sequence
Red=Cloning site Green=Tags(s) MISPAWSLFLIGTKIGLFFQVAPLSVVAKSCPSVCRCDAGFIYCNDRSLTSIPVGIPEDATTLYLQNNQI NNVGIPSDLKNLLKVQRIYLYHNSLDEFPTNLPKYVKELHLQENNIRTITYDSLSKIPYLEELHLDDNSV SAVSIEEGAFRDSNYLRLLFLSRNHLSTIPGGLPRTIEELRLDDNRISTISSPSLHGLTSLKRLVLDGNL LNNHGLGDKVFFNLVNLTELSLVRNSLTAAPVNLPGTSLRKLYLQDNHINRVPPNAFSYLRQLYRLDMSN NNLSNLPQGIFDDLDNITQLILRNNPWYCGCKMKWVRDWLQSLPVKVNVRGLMCQAPEKVRGMAIKDLSA ELFDCKDSGIVSTIQITTAIPNTAYPAQGQWPAPVTKQPDIKNPKLIKDQRTTGSPSRKTILITVKSVTP DTIHISWRLALPMTALRLSWLKLGHSPAFGSITETIVTGERSEYLVTALEPESPYRVCMVPMETSNLYLF DETPVCIETQTAPLRMYNPTTTLNREQEKEPYKNPNLPLAAIIGGAVALVSIALLALVCWYVHRNGSLFS RNCAYSKGRRRKDDYAEAGTKKDNSILEIRETSFQMLPISNEPISKEEFVIHTIFPPNGMNLYKNNLSES SSNRSYRDSGIPDSDHSHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172160 |
| ORF Size | 1950 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172160.1, NP_001165631.1 |
| RefSeq Size | 4537 bp |
| RefSeq ORF | 1950 bp |
| Locus ID | 71436 |
| UniProt ID | Q8BGT1 |
| MW | 72.9 kDa |
| Gene Summary | Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners (PubMed:19056886, PubMed:25374360). Plays a role in the spatial organization of brain neurons (PubMed:25374360). Plays a role in vascular development in the retina (PubMed:25374360). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:22405201, PubMed:25374360). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25374360). Promotes neurite outgrowth (in vitro) (By similarity). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length (By similarity). Plays a role in the regulation of the density of glutamaergic synapses (PubMed:22405201). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning (PubMed:19056886). Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development (PubMed:18448090). Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (PubMed:19056886).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209757L3 | Lenti ORF clone of Flrt3 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3), transcript variant 1 |
CNY 6,650.00 |
|
| MR209757L4 | Lenti ORF clone of Flrt3 (mGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt3), transcript variant 1 |
CNY 6,650.00 |
