Snap91 (BC052454) Mouse Tagged ORF Clone

CAT#: MR209560

  • TrueORF®

Snap91 (Myc-DDK-tagged) - Mouse synaptosomal-associated protein 91 (cDNA clone MGC:63436 IMAGE:6410678)

ORF Plasmid: DDK tGFP



  "BC052454" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,672.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AP180, F1-20, 91kDa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209560 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCCTTGAACAACATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAATGAAGGATCTGGTG
CTCCCTCTCCACTAAGTAAGTCTTCTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAAC
TATCGACACATCCCCGCCAGTTGACATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAG
CCATCAAGCGATCTCCTTGATCTTCAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAG
TGCCTCCTTCTGGGGGTGCGACCGCCTGGGGAGACCTTTTGGGAGAGGATTCCTTGGCTGCACTTTCCTC
TGTTCCCTGTGAAGCACCGATTTCAGACCCATTTGCACCAGAGCCTTCCCCTCCTACTACAACCACTGAG
CCTGCTTCAGCCTCTGCCTCGACCACCACAGCTGTGACGGCTGTCACTACGGAAGTGGATCTCTTTGGAG
ATGCCTTTGCAGCTTCTCCTGGGGAGGCCCCTGCAGCATCCGAAGGGGCTACCGCACCAGCTACCCCGGC
CCCAGTGGCTGCAGCTCTTGATGCATGCTCAGGAAATGACCCTTTTGCCCCATCTGAAGGTAGCGCAGAG
GCTGCACCTGAGCTGGACCTCTTTGCAATGAAGCCACCTGAGACCAGCGCTCCTGTAGTTACCCCTACAG
CTAGCACAGCCCCTCCAGTTCCCGCAACTGCTCCTTCTCCTGCTCCCACGGCTGTGGCAGCCACTGCTGC
CACCACCACCGCCGCCGCTGCAGCTACCACCACTGCCACCACCTCTGCTGCTGCTGCCACCACCGCCGCT
GCTCCTCCTGCTCTAGATATCTTTGGTGATTTGTTTGATTCTGCTCCTGAAGTTGCTGCAGCACCTAAGC
CAGACGCGGCTCCTAGCATAGACCTGTTTGGCACAGATGCTTTCTCCTCCCCGCCACGAGGGGCCTCTCC
GGTGCCTGAGAGTTCTCTCACTGCTGACCTCTTATCTGTGGACGCATTTGCAGCGCCGTCTCCTGCATCC
ACTGCCTCTCCTGCAAAGGCGGAGTCCTCGGGTGTCATAGACCTTTTTGGGGATGCGTTTGGAAGTGGTG
CTTCTGAAACCCAGCCAGCACCACAGGCTGTTTCTAGTTCATCAGCATCGGCAGATCTACTAGCTGGATT
TGGGGGTTCTTTCATGGCCCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCTGCTGCAACCCAGT
TTCGAGGCAGCTTTTGGAACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCATCAGTGTTTGATG
GTTTAGGCGATCTTCTGATGCCAACCATGGCACCATCCGGGCAGCCTGCCCCTGTCTCAATGGTCCCACC
CAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGGAAGTGACCTTGACTCGTCTCTGGCCAGTTTAGTAGGC
AATCTTGGGATTTCTGGTACCACATCAAAAAAGGGAGATCTCCAGTGGAATGCTGGGGAGAAAAAGCTGA
CTGGTGGAGCCAACTGGCAACCGAAAGTCACTCCAGCCACATGGTCAGCGGGTGTTCCCCCGCAAGGCAC
TGTTCCACCAACCAGCTCAGTCCCTCCGGGTGCCGGGGCCCCGTCCGTTGGGCAACCTGGAGCAGGATTT
GGAATGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCCAGTCATGTTTGCACAGCCCATGA
TGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTTTCTCCAAGCCCTACACCTGCCACTCA
GAGTCCCAAGAAACCTCCAGCCAAGGACCCGTTAGCGGATCTTAACATCAAGGATTTCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209560 protein sequence
Red=Cloning site Green=Tags(s)

METLEQHLNTLEGKKPGNNEGSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDIFATASAAAPVSSAK
PSSDLLDLQPDFSGAAAGAAAPVVPPSGGATAWGDLLGEDSLAALSSVPCEAPISDPFAPEPSPPTTTTE
PASASASTTTAVTAVTTEVDLFGDAFAASPGEAPAASEGATAPATPAPVAAALDACSGNDPFAPSEGSAE
AAPELDLFAMKPPETSAPVVTPTASTAPPVPATAPSPAPTAVAATAATTTAAAAATTTATTSAAAATTAA
APPALDIFGDLFDSAPEVAAAPKPDAAPSIDLFGTDAFSSPPRGASPVPESSLTADLLSVDAFAAPSPAS
TASPAKAESSGVIDLFGDAFGSGASETQPAPQAVSSSSASADLLAGFGGSFMAPSTTPVTPAQNNLLQPS
FEAAFGTTPSTSSSSSFDPSVFDGLGDLLMPTMAPSGQPAPVSMVPPSPAMAASKGLGSDLDSSLASLVG
NLGISGTTSKKGDLQWNAGEKKLTGGANWQPKVTPATWSAGVPPQGTVPPTSSVPPGAGAPSVGQPGAGF
GMPPSGTGMTMMSQQPVMFAQPMMRPPFGAAAVPGTQLSPSPTPATQSPKKPPAKDPLADLNIKDFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052454
ORF Size 1881 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052454, AAH52454
RefSeq Size 4519 bp
RefSeq ORF 1883 bp
Locus ID 20616
MW 60.8 kDa
Gene Summary Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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