Xpnpep1 (NM_133216) Mouse Tagged ORF Clone

CAT#: MR209521

  • TrueORF®

Xpnpep1 (Myc-DDK-tagged) - Mouse X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (Xpnpep1)

ORF Plasmid: DDK tGFP



  "NM_133216" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 4,960.00

CNY 5,510.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms D230045I08Rik; sAMP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209521 representing NM_133216
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCTCCAGAAAGCCGCCGCGAGTAAGGGCGAATCGCCAGGACTTTCAGCTGAGAAATTTAAGAA
TAATTGAACCTAACGAGGTGACACCCTCAGGAGACAAGAGTGTGGAAACAGACCACAGGATGGCTCCGAA
GGTGACTTCAGAACTGCTTCGGCAGCTGCGACAAGCTATGAGGAACTCCGAATACGTGGCAGAACCCATC
CAGGCCTATATCATCCCGTCAGGAGATGCCCACCAGAGCGAGTACATTGCACCCTGTGACTGTCGGAGGG
CGTTTGTCTCTGGATTCGATGGCTCTGCAGGCACAGCCATCATCACGGAGGAGCATGCGGCCATGTGGAC
TGACGGGCGCTACTTCCTCCAGGCTGCCAAACAGATGGACAACAACTGGACTCTCATGAAGATGGGTCTG
AAAGACACACCAACTCAGGAGGACTGGCTGGTGAGTGTGCTTCCAGAAGGATCCAGGGTTGGTGTGGACC
CCCTCATCATTCCCACAGATTACTGGAAGAAGATGGCCAAGGTACTGCGAAGTGCTGGTCACCACCTCGT
GCCTGTGAAGGAGAACCTTGTGGACAAGATCTGGACAGACCGGCCGGAGCGCCCTTGCAAGCCCCTCCTC
ACACTGGGCCTGGATTATACAGGCATCTCGTGGAAGGAGAAGGTTGCAGACCTCCGGTTGAAAATGGCAG
AGAGGAGCATCGCGTGGTTTGTAGTCACAGCCCTAGACGAGATTGCATGGCTGTTCAATCTCCGCGGGTC
AGATGTGGAACACAATCCTGTGTTTTTCTCCTATGCGATCGTAGGCCTAGAGACGATCATGCTCTTCATT
GACGGGGACCGCGTCGACGCGCCGGGCGTGAAGCAGCACCTGCTGCTTGACTTGGGCCTGGAGGCGGAAT
ACAGAATCCAGGTCCTGCCATACAAGTCCATCCTGAGTGAGCTCAAGGCCCTGTGTGCTGACCTGTCCCC
GAGGGAGAAAGTGTGGGTCAGTGACAAGGCCAGCTACGCTGTGAGCGAGGCCATCCCCAAGGATCACCGC
TGCTGTATGCCTTACACACCCATCTGCATTGCCAAAGCCGTGAAGAACTCCGCTGAGTCGGACGGCATGA
GGCGGGCTCACATTAAAGATGCCGTTGCCCTCTGCGAACTCTTCAACTGGCTGGAGCAAGAGGTTCCCAA
AGGTGGCGTTACGGAGATCTCTGCGGCTGACAAGGCCGAGGAATTTCGAAGGCAGCAGGCTGACTTTGTG
GATCTGAGCTTCCCAACGATTTCCAGCACGGGACCCAACGGCGCCATCATCCACTACGCGCCAGTCCCTG
AGACGAACAGGACCCTGTCCCTGGACGAGGTTTACCTCATTGACTCCGGTGCTCAGTACAAGGATGGAAC
CACGGACGTCACCCGGACCATGCACTTTGGGACCCCCACTGCCTATGAGAAGGAATGCTTCACATACGTA
CTGAAAGGCCACATAGCTGTGAGCGCAGCTGTTTTCCCGACGGGAACCAAAGGCCACCTTCTGGACTCCT
TTGCCCGGTCAGCCTTGTGGGATTCTGGTCTGGATTACCTGCATGGAACAGGACATGGCGTTGGGTCCTT
TTTGAATGTTCACGAGGGGCCCTGTGGCATCAGTTATAAAACATTCTCCGATGAGCCCTTGGAAGCGGGC
ATGATCGTCACTGATGAGCCAGGGTATTACGAAGACGGGGCGTTTGGGATCCGCATTGAGAATGTTGTTC
TGGTGGTCCCAGCAAAAACCAAGTATAATTTCAACAACCGAGGAAGCCTGACCTTTGAACCTCTAACTTT
GGTTCCCATCCAGACCAAAATGATAGATGTGAATGCTCTTACAGATAAAGAGTGTGACTGGCTCAACAGC
TACCACCAGACCTGCAGGGACGTGGTTGGGAAGGAGCTGCAGAGCCAGGGCCGCCAGGAAGCTCTCGAGT
GGCTCATCAGAGAGACAGAGCCTGTCTCCAGGCAGCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209521 representing NM_133216
Red=Cloning site Green=Tags(s)

MAASRKPPRVRANRQDFQLRNLRIIEPNEVTPSGDKSVETDHRMAPKVTSELLRQLRQAMRNSEYVAEPI
QAYIIPSGDAHQSEYIAPCDCRRAFVSGFDGSAGTAIITEEHAAMWTDGRYFLQAAKQMDNNWTLMKMGL
KDTPTQEDWLVSVLPEGSRVGVDPLIIPTDYWKKMAKVLRSAGHHLVPVKENLVDKIWTDRPERPCKPLL
TLGLDYTGISWKEKVADLRLKMAERSIAWFVVTALDEIAWLFNLRGSDVEHNPVFFSYAIVGLETIMLFI
DGDRVDAPGVKQHLLLDLGLEAEYRIQVLPYKSILSELKALCADLSPREKVWVSDKASYAVSEAIPKDHR
CCMPYTPICIAKAVKNSAESDGMRRAHIKDAVALCELFNWLEQEVPKGGVTEISAADKAEEFRRQQADFV
DLSFPTISSTGPNGAIIHYAPVPETNRTLSLDEVYLIDSGAQYKDGTTDVTRTMHFGTPTAYEKECFTYV
LKGHIAVSAAVFPTGTKGHLLDSFARSALWDSGLDYLHGTGHGVGSFLNVHEGPCGISYKTFSDEPLEAG
MIVTDEPGYYEDGAFGIRIENVVLVVPAKTKYNFNNRGSLTFEPLTLVPIQTKMIDVNALTDKECDWLNS
YHQTCRDVVGKELQSQGRQEALEWLIRETEPVSRQH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_133216
ORF Size 1998 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133216.3, NP_573479.3
RefSeq Size 2521 bp
RefSeq ORF 2001 bp
Locus ID 170750
UniProt ID Q6P1B1
MW 74.6 kDa
Gene Summary Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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