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Amot (BC057638) Mouse Tagged ORF Clone
CAT＃: MR209494
- TrueORF®
Amot (Myc-DDK-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191)
ORF Plasmid: tGFP
"BC057638" in other vectors (4)
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CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAG-2, mKIAA1071 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209494 representing BC057638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACACCGAGGTCCCCCGCCAGAATACCCTTTCAAGGGCGTGCCATCCCAGTCTGTAGTGTGCAAGT CCCAAGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGAT GAGGTATCAGCACCCCCCTGAGTATGGGGCAGCCAGGGCAACACAGGACATCTCCTCGCTGTCTTTGTCA GCCAGGAACTCTCAACCTCATAGCCCTACTTCTTCCCTGACAGCTGGGGCTTCCAGCTTGCCCTTGCTGC AGTCTCCGCCGTCCACTAGATTGCCTCCTGGTCAACACCTGGTCCCGAACCAAGGTGACCACTCTGCTCA CCTGTCAAGGCATCAGCAGCATCTTCTTTCGAGTCAGAGTCATCAGGGGGACCACTACCGTCACGCCCAG GCCAGCCTGACTTCAGCGCAGCAGCAGCCAGGAGAAGCCTACTCAGCTATGCCTCGGGCTCAGCAGTCTG CTTCTTATCAGCCCATGCCAGCTGACCCTTTTGCTATCGTCTCTAGAGCTCAGCAGATGGTTGAGATCCT GTCTGATGAGAATCGCAACTTGCGGCAGGAGTTGGATGGATGCTATGAGAAGGTGGCGAGACTGCAGAAG GTGGAGACAGAAATCCAACGGGTCTCAGAGGCTTATGAGAACTTGGTGAAGTCATCTTCCAAAAGAGAGG CTCTGGAGAAAGCCATGAGGAACAAGCTGGAGGGCGAGATTAGAAGGATGCATGACTTCAACAGAGATCT GAGAGACCGTCTAGAGACTGCCAACAAGCAGCTGGCAGAGAAGGAGTACGAGGGGTCCGAGGACACCAGG AAGACCATCTCGCAGCTCTTTGCCAAACATAAAGAAAACCAGCGGGAGAAGGAGAAGCTGGAAGCTGAGC TGGCTACTGCCCGTTCTACCAATGAAGACCAAAGGCGACACATTGAAATCCGAGACCAGGCATTGAGCAA TGCCCAGGCGAAGGTGGTGAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTAGATAAGGTGGAG AAGATGCAGCAGGCCCTTGTGCAGCTCCAGGCCGCCTGTGAGAAGCGTGAGCAGCTGGAACACCGGCTCC GGACACGTTTGGAGAGGGAACTGGAATCCCTTAGAATCCAGCAGCGCCAGGGCAACTCCCAGCCCACTAA TGCTTCAGAATACAATGCCGCTGCACTGATGGAGCTCCTCCGGGAGAAGGAAGAGAGAATTCTGGCCCTG GAAGCAGATATGACCAAGTGGGAGCAAAAGTATTTGGAAGAGAATGTGATGAGACACTTTGCTCTGGATG CTGCTGCAACTGTGGCTGCACAGAGAGACACAACAGTCATTAGCCACTCTCCTAACACCAGCTATGACAC AGCCTTAGAGGCCCGCATCCAGAAGGAAGAGGAAGAAATCCTGATGGCCAACAAGCGTTGCTTGGACATG GAAGGAAGGATTAAGACCCTCCATGCCCAGATTATTGAAAAGGATGCCATGATCAAGGTGCTGCAGCAGC GCTCCAGAAAGGAGCCGAGTAAAACAGAGCAACTCTCATCCATGCGGCCAGCAAAGTCTCTGATGTCCAT TTCCAATGCTGGATCAGGCCTCCTTGCATACTCCTCTACCTTGACTGGTGCGCCCATTATGGAGGAGAAG CGTGATGACAAGAGCTGGAAGGGGAGCCTAGGTAAGGAAAAGGAAAGCAACAGAAGCAAAGGCACAGTGA CAGACTTAGAAAGTGTCCTGACTCTGTTACACACAGCAAGGAAAAGGGACAATGGTCCAGGTTCTAGAGA GGAGAATTTGGAGTCCCCCCTCTCAATGGAATTAGATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209494 representing BC057638
Red=Cloning site Green=Tags(s) MEHRGPPPEYPFKGVPSQSVVCKSQEPGHFYSEHRLNQPGRTEGQLMRYQHPPEYGAARATQDISSLSLS ARNSQPHSPTSSLTAGASSLPLLQSPPSTRLPPGQHLVPNQGDHSAHLSRHQQHLLSSQSHQGDHYRHAQ ASLTSAQQQPGEAYSAMPRAQQSASYQPMPADPFAIVSRAQQMVEILSDENRNLRQELDGCYEKVARLQK VETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRDRLETANKQLAEKEYEGSEDTR KTISQLFAKHKENQREKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVE KMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNSQPTNASEYNAAALMELLREKEERILAL EADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDM EGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSSMRPAKSLMSISNAGSGLLAYSSTLTGAPIMEEK RDDKSWKGSLGKEKESNRSKGTVTDLESVLTLLHTARKRDNGPGSREENLESPLSMELDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057638 |
| ORF Size | 1860 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057638.1 |
| RefSeq Size | 2405 bp |
| RefSeq ORF | 1862 bp |
| Locus ID | 27494 |
| MW | 88.1 kDa |
| Gene Summary | Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218292 | Amot (untagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191), (10ug) |
CNY 6,940.00 |
|
| MG209494 | Amot (tGFP-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191) |
CNY 6,840.00 |
|
| MR209494L3 | Lenti ORF clone of Amot (Myc-DDK-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191) |
CNY 8,080.00 |
|
| MR209494L4 | Lenti ORF clone of Amot (mGFP-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191) |
CNY 8,080.00 |
